GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/30.10.2018

From Medien Wiki
  • Discussion about Symbiosis.
  • Presentation about projects that used microorganism. Examples with food from Christina Agapakis Bread made with death bacterias and another project with cheese and Transmediale Festival in Berlin 1000 Handshakes
  • Laboratory project, insolation of one bactería from a group of bacterias.


* Day 1: Preparation of sample

1) Medium preparation for Gluconacetobacter (500ml)

  • Glucose - 10g
  • Peptone - 2.5g
  • Yeast extract - 2.5g
  • Na2HPO4 - 1.35g
  • Citric acid - 0.75g
  • Distilled or tap water - 500ml
  • Agar - 7.5g

Bio-03.png

2) Bacteria insolation, a mixture of original bacteria kombucha diluted in distilled water in different proportions.

  • 1) 5 parts of original bacteria into 50 parts of distilled water 1/10
  • 2) 2 parts of sample #1 into 50 parts of distilled water.
  • 3) 2 parts of sample #2 into 50 parts of distilled water.
  • 4) 2 parts of sample #3 into 50 parts of distilled water.
  • 5) 2 parts of sample #4 into 50 parts of distilled water.
  • 6) Distilled water. control sample

Bio-03a.png

* Day 4: after four days the bacterias seems to be growing, all of them in white color.

Bio-03b.png

* Day 7: after one week the microorganism (bacteria) that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.

Bio-03c.png

* Day 8: In order to extract one specimen, 5 new Petri box were made like following;

1) Medium preparation for Gluconacetobacter (100ml)

  • Glucose - 2g
  • Peptone - 0.5g
  • Yeast extract - 0.5g
  • Na2HPO4 - 0.27g
  • Citric acid - 0.15g
  • Distilled or tap water - 100ml
  • Agar - 1.5g

Bio-03d.png

2) Bacteria insolation, from each sample that was made on the first day from 1 to 5 (6 was control water sample) growing bacterias were insolated like following from the original Petri-box samples;

File:Bio-03e.png original Petri-box sample day7

  • 1) 3 samples were taken from white spots.
  • 2) 1 sample is taken from a yellow spot and two samples are taken from white spots.
  • 3) 3 samples were taken from white spots with white dots (the 1 & 2 sample are not dots, they are spots).
  • 4) 1 sample is taken from a white dot and 2 samples are taken from yellow/white spots.
  • 5) 2 samples are taken from white dots and 1 sample is taken from a yellow spot.

Bio-03f.png

* Day 10: Two days after the transplantation of the samples;

  • 1) The three samples have grown into what can be seen in the following pictures.

Bio-03g.png Bio-03l.png

  • 2) The sample taken from one of the spots have grown similar to the sample in Petri box #1.

Bio-03h.png Bio-03m.png

  • 3, 4 and 5) The samples that were taken in the other three Petri sample haven shown visible results like the 2 first ones.

Bio-03i.pngBio-03j.pngBio-03k.png

Day 15: I retransplant samples from the 5 Petri dishes in order to try to insolate some new samples that have the same structure as the two first samples, and also transplant some new specimens of the two first samples in new Petri dishes.

Bio-03n.png Bio-03o.png

Day 18: I take some new clean samples of the two first samples that have an isolated specimen, the Petri dishes 3,4 and 5 didn't grow the desired specimen.

Bio-03p.png Bio-03q.png