GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/30.10.2018: Difference between revisions

From Medien Wiki
No edit summary
No edit summary
Line 5: Line 5:




* Day 1: Preparation of sample
'''* Day 1:''' Preparation of sample


1) Medium preparation for ''Gluconacetobacter'' (500ml)
1) Medium preparation for ''Gluconacetobacter'' (500ml)
Line 30: Line 30:
[[File:Bio-03a.png|400px]]
[[File:Bio-03a.png|400px]]


* Day 4: after four days the bacterias seems to be growing, all of them in white color.
'''* Day 4:''' after four days the bacterias seems to be growing, all of them in white color.


[[File:Bio-03b.png|400px]]
[[File:Bio-03b.png|400px]]


* Day 7: after one week the microorganism ''(bacteria)'' that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.
'''* Day 7:''' after one week the microorganism ''(bacteria)'' that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.


[[File:Bio-03c.png|400px]]
[[File:Bio-03c.png|400px]]


* Day 8: In order to extract one specimen, 5 new Petri box were made like following;
'''* Day 8:''' In order to extract one specimen, 5 new Petri box were made like following;


1) Medium preparation for ''Gluconacetobacter'' (100ml)
1) Medium preparation for ''Gluconacetobacter'' (100ml)
Line 64: Line 64:


[[File:Bio-03f.png|400px]]
[[File:Bio-03f.png|400px]]
'''* Day 10:''' Two days after the transplantation of the samples;
*1) The three samples have grown into what can be seen in the following pictures.
[[File:Bio-03g.png|400px]]    [[File:Bio-03l.png|400px]]
*2) The sample taken from one of the spots have grown similar to the sample in Petri box #1.
[[File:Bio-03h.png|400px]]    [[File:Bio-03m.png|400px]]
*3, 4 and 5) The samples that were taken in the other three Petri sample haven shown visible results like the 2 first ones.
[[File:Bio-03i.png|400px]][[File:Bio-03j.png|400px]][[File:Bio-03k.png|400px]]

Revision as of 20:57, 9 November 2018

  • Discussion about Symbiosis.
  • Presentation about projects that used microorganism. Examples with food from Christina Agapakis Bread made with death bacterias and another project with cheese and Transmediale Festival in Berlin 1000 Handshakes
  • Laboratory project, insolation of one bactería from a group of bacterias.


* Day 1: Preparation of sample

1) Medium preparation for Gluconacetobacter (500ml)

  • Glucose - 10g
  • Peptone - 2.5g
  • Yeast extract - 2.5g
  • Na2HPO4 - 1.35g
  • Citric acid - 0.75g
  • Distilled or tap water - 500ml
  • Agar - 7.5g

Bio-03.png

2) Bacteria insolation, a mixture of original bacteria kombucha diluted in distilled water in different proportions.

  • 1) 5 parts of original bacteria into 50 parts of distilled water 1/10
  • 2) 2 parts of sample #1 into 50 parts of distilled water.
  • 3) 2 parts of sample #2 into 50 parts of distilled water.
  • 4) 2 parts of sample #3 into 50 parts of distilled water.
  • 5) 2 parts of sample #4 into 50 parts of distilled water.
  • 6) Distilled water. control sample

Bio-03a.png

* Day 4: after four days the bacterias seems to be growing, all of them in white color.

Bio-03b.png

* Day 7: after one week the microorganism (bacteria) that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.

Bio-03c.png

* Day 8: In order to extract one specimen, 5 new Petri box were made like following;

1) Medium preparation for Gluconacetobacter (100ml)

  • Glucose - 2g
  • Peptone - 0.5g
  • Yeast extract - 0.5g
  • Na2HPO4 - 0.27g
  • Citric acid - 0.15g
  • Distilled or tap water - 100ml
  • Agar - 1.5g

Bio-03d.png

2) Bacteria insolation, from each sample that was made on the first day from 1 to 5 (6 was control water sample) growing bacterias were insolated like following from the original Petri-box samples;

File:Bio-03e.png original Petri-box sample day7

  • 1) 3 samples were taken from white spots.
  • 2) 1 sample is taken from a yellow spot and two samples are taken from white spots.
  • 3) 3 samples were taken from white spots with white dots (the 1 & 2 sample are not dots, they are spots).
  • 4) 1 sample is taken from a white dot and 2 samples are taken from yellow/white spots.
  • 5) 2 samples are taken from white dots and 1 sample is taken from a yellow spot.

Bio-03f.png

* Day 10: Two days after the transplantation of the samples;

  • 1) The three samples have grown into what can be seen in the following pictures.

Bio-03g.png Bio-03l.png

  • 2) The sample taken from one of the spots have grown similar to the sample in Petri box #1.

Bio-03h.png Bio-03m.png

  • 3, 4 and 5) The samples that were taken in the other three Petri sample haven shown visible results like the 2 first ones.

Bio-03i.pngBio-03j.pngBio-03k.png