GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/30.10.2018: Difference between revisions
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[[File:Bio-03.png|400px]] | [[File:Bio-03.png|400px]] | ||
2) Bacteria insolation, a mixture of original bacteria '' | 2) Bacteria insolation, a mixture of original bacteria ''kombucha'' diluted in distilled water in different proportions. | ||
*1) 5 parts of original bacteria into 50 parts of distilled water ''1/10'' | *1) 5 parts of original bacteria into 50 parts of distilled water ''1/10'' | ||
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* Agar - 1.5g | * Agar - 1.5g | ||
2) Bacteria insolation, from each sample that was made | [[File:Bio-03d.png|400px]] | ||
*1) | |||
2) Bacteria insolation, from each sample that was made on the first day from 1 to 5 ''(6 was control water sample)'' growing bacterias were insolated like following from the original Petri-box samples; | |||
[[:File:Bio-03e.png]] | |||
''original Petri-box sample day7'' | |||
*1) 3 samples were taken from white spots. | |||
*2) 1 sample is taken from a yellow spot and two samples are taken from white spots. | |||
*3) 3 samples were taken from white spots with white dots ''(the 1 & 2 sample are not dots, they are spots)''. | |||
*4) 1 sample is taken from a white dot and 2 samples are taken from yellow/white spots. | |||
*5) 2 samples are taken from white dots and 1 sample is taken from a yellow spot. | |||
[[File:Bio-03f.png|400px]] | |||
Revision as of 14:56, 7 November 2018
- Discussion about Symbiosis.
- Presentation about projects that used microorganism. Examples with food from Christina Agapakis Bread made with death bacterias and another project with cheese and Transmediale Festival in Berlin 1000 Handshakes
- Laboratory project, insolation of one bactería from a group of bacterias.
- Day 1: Preparation of sample
1) Medium preparation for Gluconacetobacter (500ml)
- Glucose - 10g
- Peptone - 2.5g
- Yeast extract - 2.5g
- Na2HPO4 - 1.35g
- Citric acid - 0.75g
- Distilled or tap water - 500ml
- Agar - 7.5g
2) Bacteria insolation, a mixture of original bacteria kombucha diluted in distilled water in different proportions.
- 1) 5 parts of original bacteria into 50 parts of distilled water 1/10
- 2) 2 parts of sample #1 into 50 parts of distilled water.
- 3) 2 parts of sample #2 into 50 parts of distilled water.
- 4) 2 parts of sample #3 into 50 parts of distilled water.
- 5) 2 parts of sample #4 into 50 parts of distilled water.
- 6) Distilled water. control sample
- Day 4: after four days the bacterias seems to be growing, all of them in white color.
- Day 7: after one week the microorganism (bacteria) that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.
- Day 8: In order to extract one specimen, 5 new Petri box were made like following;
1) Medium preparation for Gluconacetobacter (100ml)
- Glucose - 2g
- Peptone - 0.5g
- Yeast extract - 0.5g
- Na2HPO4 - 0.27g
- Citric acid - 0.15g
- Distilled or tap water - 100ml
- Agar - 1.5g
2) Bacteria insolation, from each sample that was made on the first day from 1 to 5 (6 was control water sample) growing bacterias were insolated like following from the original Petri-box samples;
File:Bio-03e.png original Petri-box sample day7
- 1) 3 samples were taken from white spots.
- 2) 1 sample is taken from a yellow spot and two samples are taken from white spots.
- 3) 3 samples were taken from white spots with white dots (the 1 & 2 sample are not dots, they are spots).
- 4) 1 sample is taken from a white dot and 2 samples are taken from yellow/white spots.
- 5) 2 samples are taken from white dots and 1 sample is taken from a yellow spot.
