GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/30.10.2018: Difference between revisions

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* Discussion about Symbiosis.
'''* Day 1:''' Preparation of sample
* Presentation about projects that used microorganism. Examples with food from Christina Agapakis ''Bread made with death bacterias and another project with cheese'' and Transmediale Festival in Berlin ''1000 Handshakes''
* Laboratory project, insolation of one bactería from a group of bacterias.
 
 
 
* Day 1: Preparation of sample


1) Medium preparation for ''Gluconacetobacter'' (500ml)
1) Medium preparation for ''Gluconacetobacter'' (500ml)
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* Agar - 7.5g
* Agar - 7.5g


2) Bacteria insolation, a mixture of original bacteria ''combuscha'' diluted in distilled water in different proportions.
[[File:Bio-03.png|400px]]
 
2) Bacteria insolation, a mixture of original bacteria ''kombucha'' diluted in distilled water in different proportions.


*1) 5 parts of original bacteria into 50 parts of distilled water  ''1/10''
*1) 5 parts of original bacteria into 50 parts of distilled water  ''1/10''
Line 28: Line 24:
[[File:Bio-03a.png|400px]]
[[File:Bio-03a.png|400px]]


* Day 4: after four days the bacterias seems to be growing, all of them in white color.
'''* Day 4:''' after four days the bacterias seems to be growing, all of them in white color.


[[File:Bio-03b.png|400px]]
[[File:Bio-03b.png|400px]]
'''* Day 7:''' after one week the microorganism ''(bacteria)'' that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.
[[File:Bio-03c.png|400px]]
'''* Day 8:''' In order to extract one specimen, 5 new Petri box were made like following;
1) Medium preparation for ''Gluconacetobacter'' (100ml)
* Glucose - 2g
* Peptone - 0.5g
* Yeast extract - 0.5g
* Na2HPO4 - 0.27g
* Citric acid - 0.15g
* Distilled or tap water - 100ml
* Agar - 1.5g
[[File:Bio-03d.png|400px]]
2) Bacteria insolation, from each sample that was made on the first day from 1 to 5 ''(6 was control water sample)'' growing bacterias were insolated like following from the original Petri-box samples;
[[:File:Bio-03e.png]]
''original Petri-box sample day7''
*1) 3 samples were taken from white spots.
*2) 1 sample is taken from a yellow spot and two samples are taken from white spots.
*3) 3 samples were taken from white spots with white dots ''(the 1 & 2 sample are not dots, they are spots)''.
*4) 1 sample is taken from a white dot and 2 samples are taken from yellow/white spots.
*5) 2 samples are taken from white dots and 1 sample is taken from a yellow spot.
[[File:Bio-03f.png|400px]]
'''* Day 10:''' Two days after the transplantation of the samples;
*1) The three samples have grown into what can be seen in the following pictures.
[[File:Bio-03g.png|400px]]    [[File:Bio-03l.png|400px]]
*2) The sample taken from one of the spots have grown similar to the sample in Petri box #1.
[[File:Bio-03h.png|400px]]    [[File:Bio-03m.png|400px]]
*3, 4 and 5) The samples that were taken in the other three Petri sample haven shown visible results like the 2 first ones.
[[File:Bio-03i.png|400px]][[File:Bio-03j.png|400px]][[File:Bio-03k.png|400px]]
''' Day 15:''' I retransplant samples from the 5 Petri dishes in order to try to insolate some new samples that have the same structure as the two first samples, and also transplant some new specimens of the two first samples in new Petri dishes.
[[File:Bio-03n.png|400px]]   
[[File:Bio-03o.png|400px]]
''' Day 18:''' I take some new clean samples of the two first samples that have an isolated specimen, the Petri dishes 3,4 and 5 didn't grow the desired specimen.
[[File:Bio-03p.png|400px]]
''' Day 20:''' The samples are already growing and acquiring its characteristic shape and its rosa and white color.
[[File:Bio-03q.png|400px]]
[[File:Bio-03r.png|400px]]
''' Day 27:''' I prepare some samples of the specimen in the ''lens'' in order to see them under the microscope. The results are shown in the following pictures, with the 40x the structure of the specimen can be seen, with the 100x this structure disappears to the eye, and little brown peaces can be seen.
[[File:Bio-03x.png|400px]]
[[File:Bio-03s.JPG|400px]][[File:Bio-03t.JPG|400px]][[File:Bio-03u.JPG|400px]][[File:Bio-03v.JPG|400px]][[File:Bio-03w.JPG|400px]]
New specimens are made in order to have some new clean samples.
[[File:Bio-03y.jpg|400px]]
''' Day 30:''' one of the samples taken have inside it penicillin, none of the samples before hat it before. This is a subject of study, in order to see how the isolated specimen behaves in presence of this other specimen, also there's another specimen that had contaminated all of the petri disch of one of this samples before. For the moment it can be seen, that both specimens live one next to the other without a problem.
[[File:Bio-03z.jpg|400px]]
'''POSIBLE SPECIMEN'''
Clonostachys sp.
Fusarium sp.
Acremonium masseei
Acremonium murorum
Talaromyces minioluteus
https://pdfs.semanticscholar.org/4d16/1cea571a604af97d9b05f23a3127eab92a83.pdf
https://www.interdisciplinary-laboratory.hu-berlin.de/de/content/architektur-und-morphogenese-von-biofilmen/
Gluconacetobacter aggeris
Gluconacetobacter asukensis
Gluconacetobacter azotocaptans
Gluconacetobacter diazotrophicus
Gluconacetobacter entanii
Gluconacetobacter europaeus
Gluconacetobacter hansenii
Gluconacetobacter intermedius
Gluconacetobacter johannae
Gluconacetobacter kakiaceti
Gluconacetobacter kombuchae
Gluconacetobacter liquefaciens (type species of the genus)
Gluconacetobacter maltaceti
Gluconacetobacter medellinensis
Gluconacetobacter nataicola
Gluconacetobacter oboediens
Gluconacetobacter rhaeticus
Gluconacetobacter sacchari
Gluconacetobacter saccharivorans
Gluconacetobacter sucrofermentans
Gluconacetobacter swingsii
Gluconacetobacter takamatsuzukensis
Gluconacetobacter tumulicola
Gluconacetobacter tumulisoli
Gluconacetobacter xylinus

Latest revision as of 22:04, 25 March 2019

* Day 1: Preparation of sample

1) Medium preparation for Gluconacetobacter (500ml)

  • Glucose - 10g
  • Peptone - 2.5g
  • Yeast extract - 2.5g
  • Na2HPO4 - 1.35g
  • Citric acid - 0.75g
  • Distilled or tap water - 500ml
  • Agar - 7.5g

Bio-03.png

2) Bacteria insolation, a mixture of original bacteria kombucha diluted in distilled water in different proportions.

  • 1) 5 parts of original bacteria into 50 parts of distilled water 1/10
  • 2) 2 parts of sample #1 into 50 parts of distilled water.
  • 3) 2 parts of sample #2 into 50 parts of distilled water.
  • 4) 2 parts of sample #3 into 50 parts of distilled water.
  • 5) 2 parts of sample #4 into 50 parts of distilled water.
  • 6) Distilled water. control sample

Bio-03a.png

* Day 4: after four days the bacterias seems to be growing, all of them in white color.

Bio-03b.png

* Day 7: after one week the microorganism (bacteria) that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.

Bio-03c.png

* Day 8: In order to extract one specimen, 5 new Petri box were made like following;

1) Medium preparation for Gluconacetobacter (100ml)

  • Glucose - 2g
  • Peptone - 0.5g
  • Yeast extract - 0.5g
  • Na2HPO4 - 0.27g
  • Citric acid - 0.15g
  • Distilled or tap water - 100ml
  • Agar - 1.5g

Bio-03d.png

2) Bacteria insolation, from each sample that was made on the first day from 1 to 5 (6 was control water sample) growing bacterias were insolated like following from the original Petri-box samples;

File:Bio-03e.png original Petri-box sample day7

  • 1) 3 samples were taken from white spots.
  • 2) 1 sample is taken from a yellow spot and two samples are taken from white spots.
  • 3) 3 samples were taken from white spots with white dots (the 1 & 2 sample are not dots, they are spots).
  • 4) 1 sample is taken from a white dot and 2 samples are taken from yellow/white spots.
  • 5) 2 samples are taken from white dots and 1 sample is taken from a yellow spot.

Bio-03f.png

* Day 10: Two days after the transplantation of the samples;

  • 1) The three samples have grown into what can be seen in the following pictures.

Bio-03g.png Bio-03l.png

  • 2) The sample taken from one of the spots have grown similar to the sample in Petri box #1.

Bio-03h.png Bio-03m.png

  • 3, 4 and 5) The samples that were taken in the other three Petri sample haven shown visible results like the 2 first ones.

Bio-03i.pngBio-03j.pngBio-03k.png

Day 15: I retransplant samples from the 5 Petri dishes in order to try to insolate some new samples that have the same structure as the two first samples, and also transplant some new specimens of the two first samples in new Petri dishes.

Bio-03n.png Bio-03o.png

Day 18: I take some new clean samples of the two first samples that have an isolated specimen, the Petri dishes 3,4 and 5 didn't grow the desired specimen.

Bio-03p.png

Day 20: The samples are already growing and acquiring its characteristic shape and its rosa and white color.

Bio-03q.png

Bio-03r.png

Day 27: I prepare some samples of the specimen in the lens in order to see them under the microscope. The results are shown in the following pictures, with the 40x the structure of the specimen can be seen, with the 100x this structure disappears to the eye, and little brown peaces can be seen.

Bio-03x.png

Bio-03s.JPGBio-03t.JPGBio-03u.JPGBio-03v.JPGBio-03w.JPG

New specimens are made in order to have some new clean samples.

Bio-03y.jpg

Day 30: one of the samples taken have inside it penicillin, none of the samples before hat it before. This is a subject of study, in order to see how the isolated specimen behaves in presence of this other specimen, also there's another specimen that had contaminated all of the petri disch of one of this samples before. For the moment it can be seen, that both specimens live one next to the other without a problem.

Bio-03z.jpg


POSIBLE SPECIMEN

Clonostachys sp.

Fusarium sp.


Acremonium masseei

Acremonium murorum

Talaromyces minioluteus


https://pdfs.semanticscholar.org/4d16/1cea571a604af97d9b05f23a3127eab92a83.pdf https://www.interdisciplinary-laboratory.hu-berlin.de/de/content/architektur-und-morphogenese-von-biofilmen/

Gluconacetobacter aggeris Gluconacetobacter asukensis Gluconacetobacter azotocaptans Gluconacetobacter diazotrophicus Gluconacetobacter entanii Gluconacetobacter europaeus Gluconacetobacter hansenii Gluconacetobacter intermedius Gluconacetobacter johannae Gluconacetobacter kakiaceti Gluconacetobacter kombuchae Gluconacetobacter liquefaciens (type species of the genus) Gluconacetobacter maltaceti Gluconacetobacter medellinensis Gluconacetobacter nataicola Gluconacetobacter oboediens Gluconacetobacter rhaeticus Gluconacetobacter sacchari Gluconacetobacter saccharivorans Gluconacetobacter sucrofermentans Gluconacetobacter swingsii Gluconacetobacter takamatsuzukensis Gluconacetobacter tumulicola Gluconacetobacter tumulisoli Gluconacetobacter xylinus