GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/30.10.2018: Difference between revisions

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* Distilled or tap water - 500ml
* Distilled or tap water - 500ml
* Agar - 7.5g
* Agar - 7.5g


2) Bacteria insolation, a mixture of original bacteria ''combuscha'' diluted in distilled water in different proportions.
2) Bacteria insolation, a mixture of original bacteria ''combuscha'' diluted in distilled water in different proportions.
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[[File:Bio-03c.png|400px]]
[[File:Bio-03c.png|400px]]
* Day 8: In order to extract one specimen, 5 new Petri box were made like following;
1) Medium preparation for ''Gluconacetobacter'' (100ml)
* Glucose - 2g
* Peptone - 0.5g
* Yeast extract - 0.5g
* Na2HPO4 - 0.27g
* Citric acid - 0.15g
* Distilled or tap water - 100ml
* Agar - 1.5g
2) Bacteria insolation, from each sample that was made in the first
*1)

Revision as of 13:51, 7 November 2018

  • Discussion about Symbiosis.
  • Presentation about projects that used microorganism. Examples with food from Christina Agapakis Bread made with death bacterias and another project with cheese and Transmediale Festival in Berlin 1000 Handshakes
  • Laboratory project, insolation of one bactería from a group of bacterias.


  • Day 1: Preparation of sample

1) Medium preparation for Gluconacetobacter (500ml)

  • Glucose - 10g
  • Peptone - 2.5g
  • Yeast extract - 2.5g
  • Na2HPO4 - 1.35g
  • Citric acid - 0.75g
  • Distilled or tap water - 500ml
  • Agar - 7.5g


2) Bacteria insolation, a mixture of original bacteria combuscha diluted in distilled water in different proportions.

  • 1) 5 parts of original bacteria into 50 parts of distilled water 1/10
  • 2) 2 parts of sample #1 into 50 parts of distilled water.
  • 3) 2 parts of sample #2 into 50 parts of distilled water.
  • 4) 2 parts of sample #3 into 50 parts of distilled water.
  • 5) 2 parts of sample #4 into 50 parts of distilled water.
  • 6) Distilled water. control sample

Bio-03a.png

  • Day 4: after four days the bacterias seems to be growing, all of them in white color.

Bio-03b.png

  • Day 7: after one week the microorganism (bacteria) that is wanted to be insolated, start to grow, is the white circle spots that can be clearly seen in Petri number 3, 4 and 5.

Bio-03c.png

  • Day 8: In order to extract one specimen, 5 new Petri box were made like following;

1) Medium preparation for Gluconacetobacter (100ml)

  • Glucose - 2g
  • Peptone - 0.5g
  • Yeast extract - 0.5g
  • Na2HPO4 - 0.27g
  • Citric acid - 0.15g
  • Distilled or tap water - 100ml
  • Agar - 1.5g

2) Bacteria insolation, from each sample that was made in the first

  • 1)