GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/23.10.2018/Farming 2

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  • Day 1: Farming of new specimen in Petri box. Polycephalum/physarum with sleeping specimens. The food 3 leaflets are positioned close to the specimens but not so closed in order for them to try to search the food. After the first night, they started to grow.

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  • Day 2: The specimens grow up in the darkness with a temperature of 20°C. They grew in the direction of two of the leaflets/the food, and are spreading around the Petri box.

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  • Day 4: The specimens that growth around the first two leaflets turns white, and the ones in the third leaf are yellow and growing.

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  • Day 5: There's a yellow line connecting the three places where the food was placed, around the food is appearing a white line, it could be that this Petri box is contaminated.

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  • Day 6: Inside the Petri box is a concentration of the Polycephalum is in one side of the Petri-box, the growing specimen still have the white color. I'm waiting to see if they will turn yellow.

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  • Day 7: My theory that the white specimen could turn yellow was wrong, this is just the trace of how the Polycephalum/physarum grew. The living specimen move to one corner of the Petri box, maybe running away from some contamination and is now in a sleeping state. The sample is moved to a new Petri box, inside new food/leaflets is placed in order to try to make them stay in a living/awake state again.

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  • Day 8: The completed transferred sample started to grow up, it seems healthy.

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  • Day 9: The completed transferred sample still grow up.

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  • Day 11: After two more days the specimen grow healthy and is invading the Petri box.

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  • Day 14: The specimen is moving to one side of the Petri-Box and is trying to climb in order to find food.

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  • Day 15: The Petri box is open in order to give the Polycephalum/physarum air and cultivate a new sample. The specimen is concentrating again in one said of the Petry-box. At the moment there is no contamination of other specimens.

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A subtract of food is made with leaflets dust that was situated under UV light for 10 minutes and three drops are placed inside the Petry box in order to keep the Polycephalum/physarum alive. Bio-02b12.png

  • Day 16: The specimen seems a little more yellow than before the subtract leaflets were given.

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