GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/23.10.2018/Farming 1

• Day 1: Farming of new specimen in Petri box. Polycephalum/physarum with living specimens. The food 5 leaflets are positioned really close to the specimens. After the first night, they started to grow.

• Day 2: The specimens grow up in the darkness with a temperature of 20°C. The first specimens started to grow on top of their food/leaflets.

• Day 4: Just one of the specimens in the Petri box seems to be growing out of the food.

• Day 5: The specimens continue growing out of the food in the first Petri-box. It seems that there is something else growing inside the Petri box.

• Day 6: The specimens haven´t changed their behavior, but definitely, there was something that contaminated the sample because is another specimen inside it. Will have to see what happen with both specimens.

• Day 7: In order to give the first specimen Polycephalum/physarum a chance to fight against the specimen that contaminated the sample, the Petri box where the contaminated specimen live is cleaned with alcohol and more food leaflets is placed in the Petri box. Another farming is made in order to try to rescue a sample of the original living specimen and to try to separate it from the contamination, after 6 hours they already started to grow.

• Day 8: After 1 day in the first Petri box the specimen Polycephalum/physarum started to grow up. It seems that in the second Petri box the specimen have died, and another specimen is growing. My hypothesis; I didn't took the sample in the correct form.