GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/22.01.2019

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< GMU:DIY Bio: doing things with biology‎ | Paola Stephania Calderón
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A gelelectrophoresis exercise is made in class.

is needed: - 120 vol - Agarose - Pigment (in the DNA) - Stain (to glow under the UV) it shows the quantity of base pairs. - DNA Fluoro Leiter (dna lader) We used one that reads till 1000 base points. - TAE salt solution (15 times diluted) - Distilled water - Electrodes

We prepare the medium according to the guide. WhatsApp Image 2019-01-22 at 18.52.21 (1).jpeg

Then we put a guide for the samples. WhatsApp Image 2019-01-22 at 18.52.21 (2).jpeg

We situated the electrodes one positive in one corner and one negative in the other corner. WhatsApp Image 2019-01-22 at 18.52.22.jpeg

A tube we the DNA sample is used for the test, the samples are put inside the holes made with the guide. WhatsApp Image 2019-01-22 at 18.52.21.jpeg WhatsApp Image 2019-01-22 at 18.52.22 (1).jpeg WhatsApp Image 2019-01-22 at 18.52.22 (2).jpeg

After a while the samples start to move in the saline medium because of the electrical flow created with the postive and negative poles. WhatsApp Image 2019-01-22 at 18.52.23.jpeg

Under the UV light can be seen the results of the experiment. WhatsApp Image 2019-01-22 at 19.39.11.jpeg WhatsApp Image 2019-01-22 at 19.39.11 (1).jpeg