GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/22.01.2019: Difference between revisions
(Created page with "A gelelectrophoresis exercise is made in class. is needed: - 120 vol - Agarose - Pigment (in the DNA) - Stain (to glow under the UV) it shows the quantity of base pairs. - DN...") |
No edit summary |
||
| Line 12: | Line 12: | ||
We prepare the medium according to the guide. | We prepare the medium according to the guide. | ||
[[File:WhatsApp Image 2019-01-22 at 18.52.21 (1).jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.21 (1).jpeg|400px]] | ||
Then we put a guide for the samples. | Then we put a guide for the samples. | ||
[[File:WhatsApp Image 2019-01-22 at 18.52.21 (2).jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.21 (2).jpeg|400px]] | ||
We situated the electrodes one positive in one corner and one negative in the other corner. | We situated the electrodes one positive in one corner and one negative in the other corner. | ||
[[File:WhatsApp Image 2019-01-22 at 18.52.22.jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.22.jpeg|400px]] | ||
A tube we the DNA sample is used for the test, the samples are put inside the holes made with the guide. | A tube we the DNA sample is used for the test, the samples are put inside the holes made with the guide. | ||
[[File:WhatsApp Image 2019-01-22 at 18.52.21.jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.21.jpeg|400px]] | ||
[[File:WhatsApp Image 2019-01-22 at 18.52.22 (1).jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.22 (1).jpeg|400px]] | ||
[[File:WhatsApp Image 2019-01-22 at 18.52.22 (2).jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.22 (2).jpeg|400px]] | ||
After a while the samples start to move in the saline medium because of the electrical flow created with the | |||
After a while the samples start to move in the saline medium because of the electrical flow created with the positive and negative poles. | |||
[[File:WhatsApp Image 2019-01-22 at 18.52.23.jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 18.52.23.jpeg|400px]] | ||
Under the UV light can be seen the results of the experiment. | Under the UV light can be seen the results of the experiment. | ||
[[File:WhatsApp Image 2019-01-22 at 19.39.11.jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 19.39.11.jpeg|400px]] | ||
[[File:WhatsApp Image 2019-01-22 at 19.39.11 (1).jpeg|400px]] | [[File:WhatsApp Image 2019-01-22 at 19.39.11 (1).jpeg|400px]] | ||
Revision as of 19:30, 26 January 2019
A gelelectrophoresis exercise is made in class.
is needed: - 120 vol - Agarose - Pigment (in the DNA) - Stain (to glow under the UV) it shows the quantity of base pairs. - DNA Fluoro Leiter (dna lader) We used one that reads till 1000 base points. - TAE salt solution (15 times diluted) - Distilled water - Electrodes
We prepare the medium according to the guide.
.jpeg)
Then we put a guide for the samples.
.jpeg)
We situated the electrodes one positive in one corner and one negative in the other corner.
A tube we the DNA sample is used for the test, the samples are put inside the holes made with the guide.
.jpeg)
.jpeg)
After a while the samples start to move in the saline medium because of the electrical flow created with the positive and negative poles.
Under the UV light can be seen the results of the experiment.
.jpeg)