GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

From Medien Wiki


The idea is to compare how the specimen Physarum Polycephalum grows in normal conditions, and how trained specimens behave when they have the possibility to change of salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show both experiments.

The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link: [1]


>> First approximation to the organism

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

/Farming 1 Farming from a living sample

/Farming 2 Farming from a sleeping sample

/Farming 3 Farming from a combined sample

62h Physarum Polycephalum growth video

>> Second approximation to the organism

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

/20.11.2018 Growing Polycephalum/physarum from Caicedos investigation


The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to experiment with the kombucha isolation method made before, in order to duplicate the results obtained with the coral shaped organism so I can replicate and create a method to extract/isolate this specific organism.


/Final guide

/30.10.2018 Separation of one specimen - kombucha


During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques of how to grow different kinds of specimens, observation under the microscope, between other experiments that are shown in the links below.

/16.10.2018 Introduction of the course

/06.11.2018a Growing Mycelium

/06.11.2018b Capturing electric potentials in organic matter

/18.12.2018 Observation of specimens under the microscope

/22.01.2019 Gelelectrophoresis