GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

From Medien Wiki

Documentation from experiments made in the class in order to learn how to cultivate, insolate and known the rules and process of work inside the laboratory

The blog is divided into chapters that show the different knowledge and experiments that were developed during the course.

PROJECT IDEA 1

The idea is to create an installation where people can see the traces and the movement of the specimen physarum polycephalum. I inspired myself in the installation of Leandro Erlich's Single Cloud Collection. The project consists of one layer where the specimen is going to grow from different points and describe different patrons while it moves toward the food that will be specifically situated in different points of each of the acrylic surfaces of the installation. Through this movement, the specimen leave a white almost invisible patron that can be seen over a black surface, this patron will create a contrast with the living specimen of white color that will be moving across the surface.

RELATED EXPERIMENTS

DATA sheet Physarum Polycephalum.jpg

A project developed in GMU: Experimental Microbiology class. Physarum Polycephalum in salinity environments experiment link: [1]


The first approximation to the organism

/Farming 1 Farming from a living sample

/Farming 2 Farming from a sleeping sample

/Farming 3 Farming from a combined sample

Three different samples were made the same day, with the same conditions and the same medium, but they develop in different ways, as it can be seen in the links of each experiment on top. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.


Medium experimentation base in the previous investigation of Caicedo

/20.11.2018 Growing Polycephalum/physarum from Caicedos investigation


Project idea 2

The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to experiment with the kombucha isolation method made before, in order to duplicate the results obtained with the coral shaped organism so I can replicate and create a method to extract/isolate this specific organism.

Bio-07.png

/Final guide

/30.10.2018 Separation of one specimen - kombucha

/Related organisms

Further experiments

/16.10.2018 Introduction

/06.11.2018a Growing Mycelium

/06.11.2018b Capturing electric potentials in organic matter

/18.12.2018 "Observation of specimens under the microscope"

/22.01.2019 "Gelelectrophoresis"