(113 intermediate revisions by the same user not shown)
Line 1: Line 1:
 +
==INTRODUCCTION==
 +
 +
In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.
 +
 +
 +
 +
 
==PROJECT IDEA 1==
 
==PROJECT IDEA 1==
  
The idea is to create an installation where people can see the traces and the movement of the specimen physarum polycephalum. I inspired myself in the installation of Leandro Erlich's Single Cloud Collection. The project consists of one layer where the specimen is going to grow from different points and describe different patrons while it moves toward the food that will be specifically situated in different points of each of the acrylic surfaces of the installation. Through this movement, the specimen leave a white almost invisible patron that can be seen over a black surface, this patron will create a contrast with the living specimen of white color that will be moving across the surface.
+
The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.
 +
 
 +
A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.
 +
 
 +
 
 +
[[File:62h normal medium.mp4|1500px]]
 +
 
 +
62h Physarum Polycephalum growth in neutral environment video
 +
 
 +
 
 +
[[File:52h salinity medium.mp4|1500px]]
 +
 
 +
52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''
 +
 
 +
 
 +
Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
 +
<gallery>
 +
File:0% salinity.png
 +
File:0.062% salinity.png
 +
File:0.125% salinity.png
 +
File:0.25% salinity.png
 +
File:0.5% salinity.png
 +
File:1% salinity.png
 +
</gallery>
 +
 
 +
''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link:  [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]
 +
 
  
'''RELATED EXPERIMENTS'''
+
''All experiments made in the class "Experimental Microbiology" can be seen at:''  
  
[[File:DATA sheet Physarum Polycephalum.jpg|400px]]
+
https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n
  
A project developed in GMU: Experimental Microbiology class. Physarum Polycephalum in salinity environments experiment  link: [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]
 
  
 +
RELATED EXPERIMENTS
  
[[File:Bio-02b17.png|400px]]
+
>> ''First approximation to the organism''
  
 +
The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.
  
''The first approximation to the organism''
+
Datasheet of ''Physarum Polycephalum:''
 +
 
 +
[[File:DATA sheet Physarum Polycephalum.jpg|800px]]
 +
 
 +
Links to further experiments with Physarum Polycephalum:
  
 
[[/Farming 1]]  ''Farming from a living sample''
 
[[/Farming 1]]  ''Farming from a living sample''
Line 21: Line 59:
 
[[/Farming 3]]  ''Farming from a combined sample''
 
[[/Farming 3]]  ''Farming from a combined sample''
  
Three different samples were made the same day, with the same conditions and the same medium, but they develop in different ways, as it can be seen in the links of each experiment on top. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.
 
  
 +
>> ''Second approximation to the organism''
  
 +
This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.
  
''Medium experimentation base in the previous investigation of Caicedo''
+
[[/20.11.2018]]  ''Growing Polycephalum/physarum from Caicedos investigation''
  
[[/20.11.2018]]  ''Growing Polycephalum/physarum from Caicedos investigation''
 
  
  
  
==Project idea 2==
+
==PROJECT IDEA 2==
 
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.
 
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.
  
The project idea is to experiment with the kombucha isolation method made before, in order to duplicate the results obtained with the coral shaped organism so I can replicate and create a method to extract/isolate this specific organism.  
+
The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.
 +
 
 +
[[File:Gluconacetobacter guide.jpg|800px]]
 +
 
 +
 
 +
The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.
 +
 
 +
[[/30.10.2018]]  ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.
 +
 
 +
 
  
[[File:Bio-07.png|400px]]
 
  
[[/Final guide]]
+
==FURTHER EXPERIMENTS==
  
[[/30.10.2018]]  ''Separation of one specimen - kombucha''
+
During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.
  
[[/Related organisms]]
+
''These experiments can be seen in the following links:''
  
==Further experiments==
+
[[/16.10.2018]]  ''Introduction of the course''
[[/16.10.2018]]  ''Introduction''
 
  
 
[[/06.11.2018a]]  ''Growing Mycelium''
 
[[/06.11.2018a]]  ''Growing Mycelium''
Line 51: Line 96:
 
[[/06.11.2018b]]  ''Capturing electric potentials in organic matter''
 
[[/06.11.2018b]]  ''Capturing electric potentials in organic matter''
  
[[/18.12.2018]] "Observation of specimens under the microscope"
+
[[/18.12.2018]]   ''Observation of specimens under the microscope''
  
[[/22.01.2019]] "Gelelectrophoresis"
+
[[/22.01.2019]]   ''Gelelectrophoresis''

Latest revision as of 10:51, 3 April 2019

INTRODUCCTION

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The project idea 1: (teaching Physarum Polycephalum to live in salinity mediums) is the result of the knowledge that was acquired around the selected specimen Physarum Polycephalum in the class DIY: Bio: doing things with biology and the experimentation made in the class Experimental microbiology. The project idea 2: (insolation of gluconacetobacter with coral shape) is the result of the knowledge build during the class DIY: Bio: doing things with biology. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.



PROJECT IDEA 1

The idea is to compare how the specimen Physarum Polycephalum grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.


62h Physarum Polycephalum growth in neutral environment video


52h Physarum Polycephalum growth in salinity mediums video (from 0% to 0.5% salinity)


Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.

The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMUGestaltung medialer Umgebungen: Experimental Microbiology, the experiment, and results can be seen in the following link: [1]


All experiments made in the class "Experimental Microbiology" can be seen at:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n


RELATED EXPERIMENTS

>> First approximation to the organism

The initial approximation to the specimen was made in the class: DIY BIO: doing things with biology. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of Physarum Polycephalum:

DATA sheet Physarum Polycephalum.jpg

Links to further experiments with Physarum Polycephalum:

/Farming 1 Farming from a living sample

/Farming 2 Farming from a sleeping sample

/Farming 3 Farming from a combined sample


>> Second approximation to the organism

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

/20.11.2018 Growing Polycephalum/physarum from Caicedos investigation



PROJECT IDEA 2

The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

Gluconacetobacter guide.jpg


The diary of work made in the first approximation to isolate a specimen (with coral shape) can be seen in the following link.

/30.10.2018 Separation of one specimen - kombucha Link to the first approximation to this experiment.



FURTHER EXPERIMENTS

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

These experiments can be seen in the following links:

/16.10.2018 Introduction of the course

/06.11.2018a Growing Mycelium

/06.11.2018b Capturing electric potentials in organic matter

/18.12.2018 Observation of specimens under the microscope

/22.01.2019 Gelelectrophoresis