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'''Documentation from experiments made in the class in order to learn how to cultivate, insolate and known the rules and process of work inside the laboratory'''
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==INTRODUCCTION==
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In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.
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The blog is divided into chapters that show the different knowledge and experiments that were developed during the course.
 
  
 
==PROJECT IDEA 1==
 
==PROJECT IDEA 1==
  
The idea is to create an installation where people can see the traces and the movement of the specimen physarum polycephalum. I inspired myself in the installation of Leandro Erlich's Single Cloud Collection. (that can be seen below). The project consists of three layers where the specimen is going to grow from three different points and describe a shape of its movement toward the food that will be specifically situated in different points of each of the acrylic surfaces of the installation.  
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The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.
  
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A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.
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[[File:62h normal medium.mp4|1500px]]
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62h Physarum Polycephalum growth in neutral environment video
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[[File:52h salinity medium.mp4|1500px]]
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52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''
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Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
 
<gallery>
 
<gallery>
File:psca3.jpg|inspiration
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File:0% salinity.png
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File:0.062% salinity.png
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File:0.125% salinity.png
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File:0.25% salinity.png
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File:0.5% salinity.png
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File:1% salinity.png
 
</gallery>
 
</gallery>
  
The project idea is to have 2 layers were the physarum polycephalum can grow from different opposite corners. The food will be placed on top of a square surface in different shapes so the specimen can move to it and describe different patrons while it moves. Through this movement, the specimen leave a white almost invisible patron that can be seen over a black surface, this patron will create a contrast with the living specimen of white color that will be moving across the surface.
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''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link:  [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]
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''All experiments made in the class "Experimental Microbiology" can be seen at:''
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https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n
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RELATED EXPERIMENTS
  
[[/Project: Specimen ''Physarum Polycephalum''.]]
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>> ''First approximation to the organism''
  
[[/Related organisms]]
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The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.
  
[[/23.10.2018]] ''First Farming Specimens - Polycephalum/physarum''
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Datasheet of ''Physarum Polycephalum:''
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[[File:DATA sheet Physarum Polycephalum.jpg|800px]]
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Links to further experiments with Physarum Polycephalum:
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[[/Farming 1]]  ''Farming from a living sample''
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[[/Farming 2]] ''Farming from a sleeping sample''
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[[/Farming 3]]  ''Farming from a combined sample''
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>> ''Second approximation to the organism''
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This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.
  
 
[[/20.11.2018]]  ''Growing Polycephalum/physarum from Caicedos investigation''
 
[[/20.11.2018]]  ''Growing Polycephalum/physarum from Caicedos investigation''
  
  
==Project idea 2==
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==PROJECT IDEA 2==
 
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.
 
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.
  
The project idea is to experiment with the kombucha isolation method made before, in order to duplicate the results obtained with the coral shaped organism so I can replicate and create a method to extract/isolate this specific organism.  
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The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.
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[[File:Gluconacetobacter guide.jpg|800px]]
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The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.
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[[/30.10.2018]]  ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.
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[[File:Bio-07.png|400px]]
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==FURTHER EXPERIMENTS==
  
[[/Final guide]]
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During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.
  
[[/30.10.2018]]  ''Separation of one specimen - kombucha''
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''These experiments can be seen in the following links:''
  
==Further experiments==
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[[/16.10.2018]]  ''Introduction of the course''
[[/16.10.2018]]  ''Introduction''
 
  
 
[[/06.11.2018a]]  ''Growing Mycelium''
 
[[/06.11.2018a]]  ''Growing Mycelium''
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[[/06.11.2018b]]  ''Capturing electric potentials in organic matter''
 
[[/06.11.2018b]]  ''Capturing electric potentials in organic matter''
  
[[/18.12.2018]] "Observation of specimens under the microscope"
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[[/18.12.2018]]   ''Observation of specimens under the microscope''
  
[[/22.01.2019]] "Gelelectrophoresis"
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[[/22.01.2019]]   ''Gelelectrophoresis''

Latest revision as of 10:51, 3 April 2019

INTRODUCCTION

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The project idea 1: (teaching Physarum Polycephalum to live in salinity mediums) is the result of the knowledge that was acquired around the selected specimen Physarum Polycephalum in the class DIY: Bio: doing things with biology and the experimentation made in the class Experimental microbiology. The project idea 2: (insolation of gluconacetobacter with coral shape) is the result of the knowledge build during the class DIY: Bio: doing things with biology. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.



PROJECT IDEA 1

The idea is to compare how the specimen Physarum Polycephalum grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.


62h Physarum Polycephalum growth in neutral environment video


52h Physarum Polycephalum growth in salinity mediums video (from 0% to 0.5% salinity)


Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.

The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMUGestaltung medialer Umgebungen: Experimental Microbiology, the experiment, and results can be seen in the following link: [1]


All experiments made in the class "Experimental Microbiology" can be seen at:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n


RELATED EXPERIMENTS

>> First approximation to the organism

The initial approximation to the specimen was made in the class: DIY BIO: doing things with biology. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of Physarum Polycephalum:

DATA sheet Physarum Polycephalum.jpg

Links to further experiments with Physarum Polycephalum:

/Farming 1 Farming from a living sample

/Farming 2 Farming from a sleeping sample

/Farming 3 Farming from a combined sample


>> Second approximation to the organism

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

/20.11.2018 Growing Polycephalum/physarum from Caicedos investigation



PROJECT IDEA 2

The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

Gluconacetobacter guide.jpg


The diary of work made in the first approximation to isolate a specimen (with coral shape) can be seen in the following link.

/30.10.2018 Separation of one specimen - kombucha Link to the first approximation to this experiment.



FURTHER EXPERIMENTS

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

These experiments can be seen in the following links:

/16.10.2018 Introduction of the course

/06.11.2018a Growing Mycelium

/06.11.2018b Capturing electric potentials in organic matter

/18.12.2018 Observation of specimens under the microscope

/22.01.2019 Gelelectrophoresis