GMU:DIY Bio: doing things with biology/Nezar Abualhalaweh: Difference between revisions

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The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.  
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.  
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File:Working_Conditions.JPG|Working Conditions</gallery>


Sample:  
Sample:  

Revision as of 19:41, 12 January 2019

Growing Physarum Polycephalum

Medium: 100ml water 3g agar Cooked Directly in a flask on the stove until boiling point, while consistently mixing. The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.

Sample: The Physarum Polycephalum was taken from healthy dried samples, on a paper and a paper towel.

Food source: Dried Oats (not sterilized)

Conditions: - 20 degrees temperature - enough moisture (introduced in the initial cultivation) - kept in a dark environment

Follow-up on the above Physarum Polycephalum sample in the large Petri dish

Results: The above sample was unsuccessful. Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples.


Growing Physarum Polycephalum from Living Samples

Medium: 200ml water ~2g agar The medium was prepared using a magnetic stirrer, in the lab.

The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.

Sample: The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes

Food source: Dried Oats (Sterile) The dried Oats were cooked in the Microwave for ~35 seconds.

Conditions: - 20 degrees temperature - enough moisture (introduced in the initial cultivation) - kept in a dark environment