GMU:DIY Bio: doing things with biology/Introduction to conceptual framework and tools

Safety in the lab

Goggles. Sometimes you will want to work with goggles. In case if something gets into the eyes: clean with running water!
Gloves. While working with chemicals or need clean environment for your organisms, wear gloves!
Started your work in the lab? Wash your hands!
Finished the work in the lab? Wash your hands!
No eating in the lab: you could contaminate your organisms/samples or, you could be poisoned by chemicals
Apron/lab coat. It is better to use a lab coat
Never pour chemicals back into the bottles
Never put chemicals back into the jars
Close containers immediately after pouring or taking out chemicals
Do not inhale chemicals or contaminated plates
Flame is not aloud. In case if needed to get rid of bacteria, be careful!
Read instructions before using equipment which is not familiar
Clean your work bench with 70% ethanol before and after the work
Do not put your fingers into your eyes/mouth

Conceptual framework (References)

File:Generation M .pdf Generation M Dimitris Papadopoulos
File:Moholy-nagy.pdf The New Vision Laszlo Moholy-Nagy
DIY Bio @ Hackteria, https://www.hackteria.org/

Growing microorganisms

Decision made to grow microorganisms from different surfaces. LBP (Baird-Parker liquid) recipe with sugars and proteins should be just fine for the test

LBP agar

1 liter water
1 g lactose
1 g peptone
5.1 g Na2HPO4∗7 H2O
4.2 g KH2PO4
20 g agar

References

Documentation

16.10.2018