GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata: Difference between revisions

From Medien Wiki
No edit summary
No edit summary
Line 27: Line 27:


===ISOLATION OF STREPTOMYCES===
===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
=====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment=====
::<gallery>
<gallery>
File:Soil1.png
File:Soil1.png
File:Soil.png
File:Soil.png
Line 34: Line 34:


====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
::<gallery>
<gallery>
File:NACL.png
File:NACL.png
File:Solution .png
File:Solution .png
Line 40: Line 40:


====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
:<gallery>
<gallery>
File:Diluting.png
File:Diluting.png
File:Dilutions&Solution.png
File:Dilutions&Solution.png
Line 46: Line 46:


====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
::<gallery>
<gallery>
File:Mannitol.png
File:Mannitol.png
File:Plates.png
File:Plates.png

Revision as of 06:40, 13 July 2016

Spot the antibiotics producer: STREPTOMYCES

Micro.png


Antibiotics.

The human gets ill, goes downhill, forgets the dollar bill, takes a pill, regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than the soil.

Streptomyces.


ISOLATION OF STREPTOMYCES

1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment

2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min

3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it

4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes


MSA RECIPE:

In a 250 mL conical flask mix:

  • 2g Agar
  • 2g Mannitol
  • 2g Soya flour (Holland and Barrett)
  • 10 mM CaCl2
  • 100 mL Deionised water

Medium alternative: Inorganic Salt Starch Agar (ISSA)


5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)

6th step: Incubate at 30° for 5-7 days

Incubation.png

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria


Inspiration: http://annadumitriu.tumblr.com/ - Anna Dumitriu https://exploringtheinvisible.com/ - Dr. Simon Park