Paola Stephania/LINK PROJECT - Revision history

Goji6184 at 11:52, 5 December 2018

2018-12-05T11:52:42Z

← Older revision		Revision as of 11:52, 5 December 2018
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	After one night the specimen in the ideal medium is already growing, the specimen in the middle medium is not moving like the last time ''(probably because this time the food is concentrated in the salinity medium)'' and the samples in the salinity medium seem to be dying again.		After one night the specimen in the ideal medium is already growing, the specimen in the middle medium is not moving like the last time ''(probably because this time the food is concentrated in the salinity medium)'' and the samples in the salinity medium seem to be dying again.

			[[File:wd28.jpg\|400px]]

Goji6184 at 11:52, 5 December 2018

2018-12-05T11:52:20Z

@@ Line 89: / Line 89: @@
 [[File:pp-1.jpeg|400px]]
-* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non salty medium and the one in the salty medium seems that have died.
+* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non-salty medium and the one in the salty medium seems that have died.
 [[File:pp-2.jpg|400px]]

Goji6184 at 11:28, 5 December 2018

2018-12-05T11:28:04Z

← Older revision		Revision as of 11:28, 5 December 2018
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	After 5 days the samples of ''Polycephalum/physarum'' din't came to live again.		After 5 days the samples of ''Polycephalum/physarum'' din't came to live again.

	[[File:wd23.jpg\|400px]]		[[File:wd23.jpg\|400px]]

Goji6184 at 11:27, 5 December 2018

2018-12-05T11:27:39Z

← Older revision		Revision as of 11:27, 5 December 2018
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	[[File:wd11.JPG\|400px]]		[[File:wd11.JPG\|400px]]



	* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.		* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.
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	[[File:wd12.JPG\|400px]]		[[File:wd12.JPG\|400px]]

	* Day 2: I made a new sample with a new medium~~; at left a normal medium, at~~ the ~~center a sample half with~~ the ~~normal medium and~~ the ~~other half with 3% of NaCl~~, and ~~the third sample with just the salty sample~~.		After 5 days the samples of ''Polycephalum/physarum'' din't came to live again.
			[[File:wd23.jpg\|400px]]

			* Day 2: I made a new sample with a new medium. Parallel to the samples that I use for the experiment I started to use this new Medium to grow the specimen (to have live healthy samples to experiment), I started from the formula that Fernanda Caicedo gets to in her previous experiments, and I altered for my special use.

	Medium:		Medium:
	*100 ml of water		*100 ml of water
Line 76:		Line 82:
	*(mix together)		*(mix together)
	*2 gr of agar		*2 gr of agar
	*(mix all together and ~~boiled 2 minutes~~)		*(mix all together and boil)

			At left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.

	[[File:pp-1.jpeg\|400px]]		[[File:pp-1.jpeg\|400px]]

Goji6184 at 23:35, 1 December 2018

2018-12-01T23:35:24Z

← Older revision		Revision as of 23:35, 1 December 2018
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	- Medium:		- Medium:
			*100ml of distilled water
	100ml of distilled water		*2g of agar
			*Leaflets
	2g of agar

	Leaflets


Line 48:		Line 45:

	- Medium:		- Medium:
			*NaCl 30 g
	NaCl 30 g		*Glycerol 1 g
			*Pepton (Bacxto-peptone) 10 g
	Glycerol 1 g		*Meat extract 3 g
			*Made up with distilled water to 1000 ml
	Pepton (Bacxto-peptone) 10 g

	Meat extract 3 g

	Made up with distilled water to 1000 ml


Line 68:		Line 60:

	* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.		* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.


	CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.		CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.

Goji6184 at 23:34, 1 December 2018

2018-12-01T23:34:26Z

← Older revision		Revision as of 23:34, 1 December 2018
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	[[File:pp-2.jpg\|400px]]		[[File:pp-2.jpg\|400px]]

	~~hoober phonic~~

Goji6184 at 23:34, 1 December 2018

2018-12-01T23:34:07Z

← Older revision		Revision as of 23:34, 1 December 2018
Line 28:		Line 28:
	- Medium:		- Medium:

	*100ml of distilled water		100ml of distilled water
	*2g of agar
	*Leaflets		2g of agar

			Leaflets


Line 46:		Line 48:

	- Medium:		- Medium:
	*NaCl 30 g
	*Glycerol 1 g		NaCl 30 g
	*Pepton (Bacxto-peptone) 10 g
	*Meat extract 3 g		Glycerol 1 g
	*Made up with distilled water to 1000 ml
			Pepton (Bacxto-peptone) 10 g

			Meat extract 3 g

			Made up with distilled water to 1000 ml



Line 60:		Line 68:

	* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.		* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.


	CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.		CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.




	'''EXPERIMENTATION with ''Polycephalum/physarum'' '''		'''EXPERIMENTATION with ''Polycephalum/physarum'' '''

	* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''.		* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''.

Line 74:		Line 77:

	* Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.		* Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.

	Medium:		Medium:
	*100 ml of water		*100 ml of water

Goji6184 at 23:33, 1 December 2018

2018-12-01T23:33:56Z

← Older revision		Revision as of 23:33, 1 December 2018
Line 28:		Line 28:
	- Medium:		- Medium:

	100ml of distilled water		*100ml of distilled water
			*2g of agar
	2g of agar		*Leaflets

	Leaflets


Line 48:		Line 46:

	- Medium:		- Medium:
			*NaCl 30 g
	NaCl 30 g		*Glycerol 1 g
			*Pepton (Bacxto-peptone) 10 g
	Glycerol 1 g		*Meat extract 3 g
			*Made up with distilled water to 1000 ml
	Pepton (Bacxto-peptone) 10 g

	Meat extract 3 g

	Made up with distilled water to 1000 ml



Line 68:		Line 60:

	* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.		* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.


	CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.		CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.




	'''EXPERIMENTATION with ''Polycephalum/physarum'' '''		'''EXPERIMENTATION with ''Polycephalum/physarum'' '''

	* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''.		* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''.

Line 77:		Line 74:

	* Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.		* Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.

	Medium:		Medium:
	*100 ml of water		*100 ml of water

Goji6184 at 23:31, 1 December 2018

2018-12-01T23:31:50Z

← Older revision		Revision as of 23:31, 1 December 2018
Line 85:		Line 85:
	*(mix all together and boiled 2 minutes)		*(mix all together and boiled 2 minutes)

			[[File:pp-1.jpeg\|400px]]

	* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non salty medium and the one in the salty medium seems that have died.		* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non salty medium and the one in the salty medium seems that have died.

Goji6184 at 23:26, 1 December 2018

2018-12-01T23:26:01Z

← Older revision		Revision as of 23:26, 1 December 2018
Line 76:		Line 76:
	[[File:wd12.JPG\|400px]]		[[File:wd12.JPG\|400px]]

	* Day 2:		* Day 2: I made a new sample with a new medium; at left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.
			Medium:
			*100 ml of water
			*4 gr of cooked potatoes
			*4 gr of oat flakes
			*(mix together)
			*2 gr of agar
			*(mix all together and boiled 2 minutes)



			* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non salty medium and the one in the salty medium seems that have died.

			[[File:pp-2.jpg\|400px]]

	hoober phonic		hoober phonic