GMU:Life in an aquatic ecosystem/Dahye Seo - Revision history

Dahyeseo at 16:53, 6 January 2025

2025-01-06T16:53:05Z

← Older revision		Revision as of 16:53, 6 January 2025
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	</gallery>DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber		</gallery>DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber

			* �Extra Guide of Electrophoresis https://blog.naver.com/kmkae52/222804356219<br />
	=====2. Sound part=====		=====2. Sound part=====
	1) Sonification of DNA movementMax/Msp patch		1) Sonification of DNA movementMax/Msp patch
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	2) Sound Design		2) Sound Design

			===== *reference : Paul '''''Latent Figure Protocol'', Paul Vanouse, 2007-09''' https://www.paulvanouse.com/lfp.html =====

	====Work in Biolab====		====Work in Biolab====

Dahyeseo at 13:01, 6 January 2025

2025-01-06T13:01:45Z

← Older revision		Revision as of 13:01, 6 January 2025
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	'''Dead Life Live Life''' is a poetic exploration of the interconnectedness of life and death through the ~~sound and movement~~ of DNA. ~~This work~~ sonifies the DNA movement of a persimmon tree planted at my grandmother's house, who passed away in 2018. The tree serves as a living memory of her, still enduring in the now-empty house. The project visualizes the movement of the tree’s DNA through electrophoresis experiments, converting this movement into sound in real time.		'''Dead Life Live Life''' is a poetic exploration of the interconnectedness of life and death through the sonification of the DNA movement.� It sonifies the DNA movement of a persimmon tree planted at my grandmother's house, who passed away in 2018. The tree serves as a living memory of her, still enduring in the now-empty house. The project visualizes the movement of the tree’s DNA through electrophoresis experiments, converting this movement into sound in real time.

	[[File:Diagram Dead LifeLiveLife.jpg\|center\|thumb\|800x800px]]		[[File:Diagram Dead LifeLiveLife.jpg\|center\|thumb\|800x800px]]

Dahyeseo at 11:47, 5 January 2025

2025-01-05T11:47:55Z

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	''Dead Life Live Life'' is a sound ~~installation work that sonifies the~~ movement of DNA. This work ~~was inspired by~~ the ~~poetic~~ movement of ~~DNA in electrophoresis experiments.~~ '~~'Dead Life Live Life'' explores the materiality of DNA itself rather than genetic analysis~~, ~~which is the general purpose of DNA~~. ~~“Dead life got the same material~~ as ~~live life”~~, ~~biologist Lynn Margulis said~~ in ~~an interview. This could be paraphrased as “Dead life got~~ the ~~same DNA as live life”~~. ~~''Dead Life Live Life'' poetically represents~~ the ~~connection between life and death by sonifying~~ the DNA movement ~~of an old persimmon tree planted~~ in ~~my grandmother's house~~.		'''Dead Life Live Life''' is a poetic exploration of the interconnectedness of life and death through the sound and movement of DNA. This work sonifies the DNA movement of a persimmon tree planted at my grandmother's house, who passed away in 2018. The tree serves as a living memory of her, still enduring in the now-empty house. The project visualizes the movement of the tree’s DNA through electrophoresis experiments, converting this movement into sound in real time.

	[[File:Diagram Dead LifeLiveLife.jpg\|center\|thumb\|800x800px]]		[[File:Diagram Dead LifeLiveLife.jpg\|center\|thumb\|800x800px]]

Dahyeseo at 15:55, 18 December 2024

2024-12-18T15:55:48Z

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	During the PCR experiment, I successfully amplified all the sample DNA and proceeded with gel electrophoresis. However, I encountered an error message indicating that no electricity was flowing through the gel, which prevented me from continuing the experiment.		During the PCR experiment, I successfully amplified all the sample DNA and proceeded with gel electrophoresis. However, I encountered an error message indicating that no electricity was flowing through the gel, which prevented me from continuing the experiment.

			===== '''2024.12.17. Tue''' =====
			<u>Introduction</u>

			Electrophoresis was performed under constant UV light and the movement of the dna was filmed live. Experiments with DNA Ladder.

			<u>Result</u>

			<nowiki>https://youtu.be/OsDsI0Ecbg4?si=UxQGSdfn7OdSeWgl</nowiki>

			Under constant UV light, the DNA becomes increasingly blurry. I feel that it is not against the artistic message of this work that the DNA gradually disappears as an inherent property during the experiment.

Dahyeseo at 10:17, 16 December 2024

2024-12-16T10:17:58Z

← Older revision		Revision as of 10:17, 16 December 2024
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	I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives.		I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives.

			===== '''2024.12.11''' =====
	'''2024.12.11'''
	[[File:Pcr 29241211.jpg\|thumb]]		[[File:Pcr 29241211.jpg\|thumb]]
	<u>Introduction</u>		<u>Introduction</u>
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	I did PCR 3 samples which are DNA of leaves extracted by DNA EXTRACT KIT, Banana, Blueberry.		I did PCR 3 samples which are DNA of leaves extracted by DNA EXTRACT KIT, Banana, Blueberry.


			<u>Result</u>

			During the PCR experiment, I successfully amplified all the sample DNA and proceeded with gel electrophoresis. However, I encountered an error message indicating that no electricity was flowing through the gel, which prevented me from continuing the experiment.

Dahyeseo at 11:26, 11 December 2024

2024-12-11T11:26:08Z

← Older revision		Revision as of 11:26, 11 December 2024
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	I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives.		I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives.


			'''2024.12.11'''
			[[File:Pcr 29241211.jpg\|thumb]]
			<u>Introduction</u>

			PCR / Gel electrophoresis

			I did PCR 3 samples which are DNA of leaves extracted by DNA EXTRACT KIT, Banana, Blueberry.

Dahyeseo: /* 2024.11.20. Wed */

2024-12-09T21:51:59Z

2024.11.20. Wed

← Older revision		Revision as of 21:51, 9 December 2024
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	<u>Introduction</u>		<u>Introduction</u>

	DNA Extraction Exercise_ Kaki ~~(ChatGPT)~~, Leaf		DNA Extraction Exercise_ Kaki, Leaf

	- I failed to extract Kaki's DNA in my experiment yesterday. This time i followed ~~Chat GPT's~~ guide to extract Kaki's DNA.		- I failed to extract Kaki's DNA in my experiment yesterday. This time i followed the guide from the result of research by Alessandro to extract Kaki's DNA.

	- Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside.		- Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside.
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	-The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf.		-The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf.

	~~<nowiki>*</nowiki>Chat GPT's guide~~

	<small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small>		<small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small>
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	Both the leaves and the persimmon failed to extract DNA. I was a little frustrated.		Both the leaves and the persimmon failed to extract DNA. I was a little frustrated.

	~~The guide for chat GPT seems unreliable.~~ It seems like I need to extract DNA from the leaves persimmon tree.		It seems like I need to extract DNA from the leaves persimmon tree.

	But where do I get the leaves?<gallery>		But where do I get the leaves?<gallery>

Dahyeseo at 16:56, 9 December 2024

2024-12-09T16:56:25Z

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	{{#ev:youtube\|bGzmhJ9kQi4}}		{{#ev:youtube\|bGzmhJ9kQi4}}

	=== '''Dead Life Live Life''' ===		=== '''Dead Life Live Life (working title)''' ===

Dahyeseo at 16:56, 9 December 2024

2024-12-09T16:56:05Z

← Older revision		Revision as of 16:56, 9 December 2024
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	''Dead Life Live Life'' is a sound installation work that sonifies the movement of DNA. This work was inspired by the poetic movement of DNA in electrophoresis experiments. ''Dead Life Live Life'' explores the materiality of DNA itself rather than genetic analysis, which is the general purpose of DNA. “Dead life got the same material as live life”, biologist Lynn Margulis said in an interview. This could be paraphrased as “Dead life got the same DNA as live life”. ''Dead Life Live Life'' poetically represents the connection between life and death by sonifying the DNA movement of an old persimmon tree planted in my grandmother's house.		''Dead Life Live Life'' is a sound installation work that sonifies the movement of DNA. This work was inspired by the poetic movement of DNA in electrophoresis experiments. ''Dead Life Live Life'' explores the materiality of DNA itself rather than genetic analysis, which is the general purpose of DNA. “Dead life got the same material as live life”, biologist Lynn Margulis said in an interview. This could be paraphrased as “Dead life got the same DNA as live life”. ''Dead Life Live Life'' poetically represents the connection between life and death by sonifying the DNA movement of an old persimmon tree planted in my grandmother's house.

			[[File:Diagram Dead LifeLiveLife.jpg\|center\|thumb\|800x800px]]

	[[File:Dlll 1.001.jpg\|center\|frameless\|800x800px]]		[[File:Dlll 1.001.jpg\|center\|frameless\|800x800px]]
	[[File:Dlll 2..jpg\|center\|frameless\|800x800px]]		[[File:Dlll 2..jpg\|center\|frameless\|800x800px]]
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	~~[[File:Diagram Dead LifeLiveLife.jpg\|center\|thumb\|800x800px]]~~

	==== Technical solution ====		====Technical solution====

	===== 1. Biological part =====		=====1. Biological part=====
	Instruction of DNA analysis<gallery>		Instruction of DNA analysis<gallery>
	File:Instruction1 DNAworkshop.jpg		File:Instruction1 DNAworkshop.jpg
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			=====2. Sound part=====
	===== 2. Sound part =====
	1) Sonification of DNA movementMax/Msp patch		1) Sonification of DNA movementMax/Msp patch

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	2) Sound Design		2) Sound Design

	==== Work in Biolab ====		====Work in Biolab====

	===== '''2024.11.16. Sat''' =====		====='''2024.11.16. Sat'''=====
	<u>Introduction</u>		<u>Introduction</u>

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	<gallery>File:After 50hours gelElectrophoresis.jpg</gallery>		<gallery>File:After 50hours gelElectrophoresis.jpg</gallery>

	===== ''' 2024.11.18. Mon''' =====		=====''' 2024.11.18. Mon'''=====
	<u>Introduction</u>		<u>Introduction</u>

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	<gallery> File:DNALadder UV lighting.jpg </gallery>		<gallery> File:DNALadder UV lighting.jpg </gallery>

	===== '''2024.11.19. Tue''' =====		====='''2024.11.19. Tue'''=====
	<u>Introduction</u>		<u>Introduction</u>

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	</gallery>		</gallery>

	===== '''2024.11.20. Wed''' =====		====='''2024.11.20. Wed'''=====
	<u>Introduction</u>		<u>Introduction</u>

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	</gallery>		</gallery>

	===== '''2024.11.21. Thu''' =====		====='''2024.11.21. Thu'''=====
	<u>Introduction</u>		<u>Introduction</u>

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	I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR.		I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR.

	# Centrifuge setting: 8kg, 60 seconds		#Centrifuge setting: 8kg, 60 seconds
	# PCR: see instructional photo above.		#PCR: see instructional photo above.

	A total of five DNA samples were subjected to DNA amplification by PCR		A total of five DNA samples were subjected to DNA amplification by PCR
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	*#B2: DNA of Blueberries (from 21.Nov)		*#B2: DNA of Blueberries (from 21.Nov)
	*#BA: DNA of Banana (from 21.Nov)		*#BA: DNA of Banana (from 21.Nov)
	*#K: Kaki pulp with soap, salt, hot water (from 20.Nov)		*# K: Kaki pulp with soap, salt, hot water (from 20.Nov)

	<small>*sample B11, B12 are dirtier than B2</small>		<small>*sample B11, B12 are dirtier than B2</small>
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	<nowiki></gallery>		<nowiki></gallery>

	===== '''2024.11.26. Tue''' =====		====='''2024.11.26. Tue'''=====
	<u>Introduction</u>		<u>Introduction</u>

Dahyeseo at 16:54, 9 December 2024

2024-12-09T16:54:53Z

← Older revision		Revision as of 16:54, 9 December 2024
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			{{#ev:youtube\|bGzmhJ9kQi4}}

	=== '''Dead Life Live Life''' ===		=== '''Dead Life Live Life''' ===


	~~{{#ev:youtube\|bGzmhJ9kQi4}}~~