GMU:Experimental Microbiology/Amoeba proteus - Revision history

MariaDegand at 09:12, 26 March 2019

2019-03-26T09:12:38Z

← Older revision		Revision as of 09:12, 26 March 2019
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	=== Observations ===		=== Observations ===
			Observations

			Water clarity is the main concern when working with amoeba proteus. In chamber 4 with compost soil, we probably had some amoeba but could not find them. It should be possible to identify new amoeba proteus with the bare eye. In dirty water or water with wheat particles in them, it was not possible to do this simple observation.

			Magnification when using the microscope. We found our first Amoeba proteus in cultivation with a 60 magnification. (Small scale Magnification)
			(Video) Later we accelerated some of the video footage and found several other, but smaller Amoeba. (Video II with notes and descriptions) We assume far more amoeba in the chambers than we found.

			Habitat for us is flat, for amoeba very three dimensional. Amoeba is commonly found at the bottom/ Top of the habitat. In order to find a moving Amoeba, the preparation needs to stay still under the microscope for at least 5 minutes. Turn the lighting of the microscope off and do not move the microscope as amoeba will be in the Cyst state after preparing it.

			The Organism is fascinating and a good subject for studying cell organelles and plasmodia behavior. In Chamber 4 we expected to find amoeba in a more natural less constructed environment. We could find Amoeba in all Chambers but the biggest and most active Amoeba in Chamber 5. Furthermore, we were able to keep Amoeba alive in a Jar for several weeks without much maintenance. (Picture)

			Projects with Amoeba might be about sensitivity, basic biology, and microscopy.

			=== Other Projects/ Pages by Media Art about Amoeba Proteus ===
			https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:Bio-Lab/Amoeba

			https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/euglena,_amoeba

Junilia at 20:53, 10 November 2018

2018-11-10T20:53:31Z

← Older revision		Revision as of 20:53, 10 November 2018
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	by Antje Danz & Maria Degand		by Antje Danz & Maria Degand
	== Culturing "general" ==		== Culturing "general" ==
	Our ~~Websearch~~:		Our Web researching:
	Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in.		Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in.

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	Rice + Soil decoction in Volvic Water		Rice + Soil decoction in Volvic Water

	=== ~~Cultur tests~~ ===		=== Culture medium testing ===
	==== Wheat ====		==== Wheat grains====
	[[File:WhatsApp Image 2018-11-09 at 17.40.32(4).jpeg\|400px\|right]]		[[File:WhatsApp Image 2018-11-09 at 17.40.32(4).jpeg\|400px\|right]]
	1. Wheat (crushed) + Distilled ~~Water~~, boil, cool down and put Amoeba proteus in.		1. Wheat grains (crushed) + Distilled water, boil, cool down and put Amoeba proteus in.
	Take wheat out before it starts to mould.		Take wheat grains out before it starts to mould.

	2. Volvic ~~Water and wheat~~ (crushed) ~~and put~~ Amoeba proteus in.		2. Volvic water andwheat grains (crushed) inoculate with Amoeba proteus.
	Take wheat out before it starts to mould.		Take wheat grains out before it starts to mould.

	( IDEA: Pond water (Ilm water from behind mensa) and wheat (crushed) ~~and put~~ Amoeba proteus in.		( IDEA: Pond water (Ilm water from behind mensa) and wheat (crushed) inoculate with Amoeba proteus.
	Take wheat out before it starts to mould. )		Take wheat grains out before it starts to mould. )

	==== Rice ====		==== Rice grains ====
	3. ~~Rice + Soil~~ (~~Heilerde~~) + Water + Amoebe		3. Grains of rice (uncooked) + healing clay + Water + Amoebe proteus

	4. ~~Rice~~ + ~~Komposterde~~ + Water + ~~Amoebe~~		4. Grains of rice (uncooked) + compost soil + Water + Amoeba proteus

	==== Extra ====		==== Extra ====

	5. ~~Wheat-Water~~ + ~~Coffee~~		5. Decoction of wheat + drip brew

	6. ~~Wheat-Water~~ + ~~Wheat~~ + Algae Euglena Mix		6. Decoction of wheat + whole wheat grains + Algae Euglena Mix

	=== Observations ===		=== Observations ===


	=== ~~Sources~~ ===		=== Resources ===
	Biology Discussion. (2018). Amoeba Proteus: Habitat, Structure and Metabolism. [online] Available at: http://www.biologydiscussion.com/invertebrate-zoology/protozoa/amoeba-proteus-habitat-structure-and-metabolism/28183 [Accessed 9 Nov. 2018].		Biology Discussion. (2018). Amoeba Proteus: Habitat, Structure and Metabolism. [online] Available at: http://www.biologydiscussion.com/invertebrate-zoology/protozoa/amoeba-proteus-habitat-structure-and-metabolism/28183 [Accessed 9 Nov. 2018].

	Helbig, G. (2018). Lebendkulturen Helbig. [online] Lebendkulturen.de. Available at: http://lebendkulturen.de/index.php/kulturen/amoeba-proteus [Accessed 9 Nov. 2018].		Helbig, G. (2018). Lebendkulturen Helbig. [online] Lebendkulturen.de. Available at: http://lebendkulturen.de/index.php/kulturen/amoeba-proteus [Accessed 9 Nov. 2018].

MariaDegand at 17:05, 9 November 2018

2018-11-09T17:05:42Z

← Older revision		Revision as of 17:05, 9 November 2018
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			by Antje Danz & Maria Degand
	== Culturing "general" ==		== Culturing "general" ==
	Our Websearch:		Our Websearch:

MariaDegand: /* Cultur tests */

2018-11-09T16:58:38Z

Cultur tests

← Older revision		Revision as of 16:58, 9 November 2018
Line 23:		Line 23:

	4. Rice + Komposterde + Water + Amoebe		4. Rice + Komposterde + Water + Amoebe

			==== Extra ====

			5. Wheat-Water + Coffee

			6. Wheat-Water + Wheat + Algae Euglena Mix

	=== Observations ===		=== Observations ===

MariaDegand: /* Culturing "general" */

2018-11-09T16:51:02Z

Culturing "general"

← Older revision		Revision as of 16:51, 9 November 2018
Line 1:		Line 1:

	== Culturing "general" ==		== Culturing "general" ==
			Our Websearch:
	Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in.		Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in.

			What the producer wrote:
			Rice + Soil decoction in Volvic Water

	=== Cultur tests ===		=== Cultur tests ===
	==== Wheat ====		==== Wheat ====
			[[File:WhatsApp Image 2018-11-09 at 17.40.32(4).jpeg\|400px\|right]]
	1. Wheat (crushed) + Distilled Water, boil, cool down and put Amoeba proteus in.		1. Wheat (crushed) + Distilled Water, boil, cool down and put Amoeba proteus in.
	Take wheat out before it starts to mould.		Take wheat out before it starts to mould.
Line 13:		Line 16:
	Take wheat out before it starts to mould.		Take wheat out before it starts to mould.

	3. Pond water (Ilm water from behind mensa) and wheat (crushed) and put Amoeba proteus in.		( IDEA: Pond water (Ilm water from behind mensa) and wheat (crushed) and put Amoeba proteus in.
	Take wheat out before it starts to mould.		Take wheat out before it starts to mould. )

	==== Rice ====		==== Rice ====
	1. Rice + Soil (Heilerde) + Water + Amoebe		3. Rice + Soil (Heilerde) + Water + Amoebe

	2. Rice + Komposterde + Water + Amoebe		4. Rice + Komposterde + Water + Amoebe

	=== Observations ===		=== Observations ===

MariaDegand: /* Cultur tests */

2018-11-09T16:14:54Z

Cultur tests

← Older revision		Revision as of 16:14, 9 November 2018
Line 6:		Line 6:

	=== Cultur tests ===		=== Cultur tests ===
			==== Wheat ====
	1. Wheat (crushed) + Distilled Water, boil, cool down and put Amoeba proteus in.		1. Wheat (crushed) + Distilled Water, boil, cool down and put Amoeba proteus in.
	Take wheat out before it starts to mould.		Take wheat out before it starts to mould.
Line 14:		Line 14:

	3. Pond water (Ilm water from behind mensa) and wheat (crushed) and put Amoeba proteus in.		3. Pond water (Ilm water from behind mensa) and wheat (crushed) and put Amoeba proteus in.
	Take wheat out before it starts to mould.		Take wheat out before it starts to mould.

			==== Rice ====
			1. Rice + Soil (Heilerde) + Water + Amoebe

			2. Rice + Komposterde + Water + Amoebe

	=== Observations ===		=== Observations ===

MariaDegand at 15:45, 9 November 2018

2018-11-09T15:45:10Z

← Older revision		Revision as of 15:45, 9 November 2018
Line 17:		Line 17:

	=== Observations ===		=== Observations ===


			=== Sources ===
			Biology Discussion. (2018). Amoeba Proteus: Habitat, Structure and Metabolism. [online] Available at: http://www.biologydiscussion.com/invertebrate-zoology/protozoa/amoeba-proteus-habitat-structure-and-metabolism/28183 [Accessed 9 Nov. 2018].

			Helbig, G. (2018). Lebendkulturen Helbig. [online] Lebendkulturen.de. Available at: http://lebendkulturen.de/index.php/kulturen/amoeba-proteus [Accessed 9 Nov. 2018].

MariaDegand: Created page with " == Culturing "general" == Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in. === Cultur tests === 1. Wheat (crushed) + Distilled Water, boil, cool down..."

2018-11-09T14:08:59Z

Created page with " == Culturing "general" == Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in. === Cultur tests === 1. Wheat (crushed) + Distilled Water, boil, cool down..."

New page

== Culturing "general" ==

Wheat + Distilled Water, Boil, cool down and put Amoeba proteus in.

=== Cultur tests ===

1. Wheat (crushed) + Distilled Water, boil, cool down and put Amoeba proteus in.
Take wheat out before it starts to mould.

2. Volvic Water and wheat (crushed) and put Amoeba proteus in.
Take wheat out before it starts to mould.

3. Pond water (Ilm water from behind mensa) and wheat (crushed) and put Amoeba proteus in.
Take wheat out before it starts to mould.

=== Observations ===