GMU:DIY Biolab License SS23 - Revision history

Leandra Schieder: /* Day 1: (6h) */

2023-04-19T08:53:32Z

Day 1: (6h)

← Older revision		Revision as of 08:53, 19 April 2023
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	[[Documentation- Day 1 / 17.04.2023]]		[[Documentation- Day 1 / 17.04.2023]]

	After Alessandro explained the rules in the laboratory and the different functions of the equipment to us, we were now allowed to start working ourselves. We prepared our work materials, for which we needed a jar with a perforated lid, a bit of cotton wool, a scale, a petri dish, a measuring cup, as well as malt extract, distilled water, ~~and agar agar powder~~.		After Alessandro explained the rules in the laboratory and the different functions of the equipment to us, we were now allowed to start working ourselves. We prepared our work materials, for which we needed a jar with a perforated lid, a bit of cotton wool, a scale, a petri dish, a measuring cup, as well as malt extract, distilled water,
	~~(Photos of the Equipment )~~		[[File:PXL_20230417_095611944.jpg]]

	We were also given a table that allowed us to determine the perfect ratio of ingredients for a perfect nutrient medium		We were also given a table that allowed us to determine the perfect ratio of ingredients for a perfect nutrient medium
	~~(photos table)~~		[[File:PXL_20230417_095240009.jpg]]

	We mixed 0.2 g of malt and 0.2 g of agar agar with 10 ml of liquid. Everything was mixed together and put into the jar.		We mixed 0.2 g of malt and 0.2 g of agar agar with 10 ml of liquid. Everything was mixed together and put into the jar.
	The malt extract mixed with agar agar and distilled water provides a perfect, soft nutrient medium for the organisms.		The malt extract mixed with agar agar and distilled water provides a perfect, soft nutrient medium for the organisms.
			[[File:PXL_20230417_100729061.jpg]][[File:PXL_20230417_100527151.jpg]][[File:PXL_20230417_100331363.jpg]]

	~~(Photos jar)~~		[[File:PXL_20230417_101010034.jpg]]

	Next, we needed to sterilize everything inside and outside of the jar, so we placed the lids (equipped with cotton wool in the perforation to prevent any mold from getting inside) into the pressure cooker. One of the jars was marked with tape that would change its color, so we would know when the pressure cooker had finished its sterilization process.		Next, we needed to sterilize everything inside and outside of the jar, so we placed the lids (equipped with cotton wool in the perforation to prevent any mold from getting inside) into the pressure cooker. One of the jars was marked with tape that would change its color, so we would know when the pressure cooker had finished its sterilization process.

	~~(photos tape, jars in cooker)~~

	After the jars were finished, we noticed the first changes. Due to the heat, the agar agar had turned from a liquid to a solid form. Now, we were able to inoculate our nutrient medium with the mushrooms.		After the jars were finished, we noticed the first changes. Due to the heat, the agar agar had turned from a liquid to a solid form. Now, we were able to inoculate our nutrient medium with the mushrooms.

Leandra Schieder: /* Day 1: (6h) */

2023-04-19T08:49:02Z

Day 1: (6h)

← Older revision		Revision as of 08:49, 19 April 2023
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	After Alessandro explained the rules in the laboratory and the different functions of the equipment to us, we were now allowed to start working ourselves. We prepared our work materials, for which we needed a jar with a perforated lid, a bit of cotton wool, a scale, a petri dish, a measuring cup, as well as malt extract, distilled water, and agar agar powder.		After Alessandro explained the rules in the laboratory and the different functions of the equipment to us, we were now allowed to start working ourselves. We prepared our work materials, for which we needed a jar with a perforated lid, a bit of cotton wool, a scale, a petri dish, a measuring cup, as well as malt extract, distilled water, and agar agar powder.
	(Photos of the Equipment )		(Photos of the Equipment )

	We were also given a table that allowed us to determine the perfect ratio of ingredients for a perfect nutrient medium		We were also given a table that allowed us to determine the perfect ratio of ingredients for a perfect nutrient medium
	(photos table)		(photos table)

	We mixed 0.2 g of malt and 0.2 g of agar agar with 10 ml of liquid. Everything was mixed together and put into the jar.		We mixed 0.2 g of malt and 0.2 g of agar agar with 10 ml of liquid. Everything was mixed together and put into the jar.
	The malt extract mixed with agar agar and distilled water provides a perfect, soft nutrient medium for the organisms.		The malt extract mixed with agar agar and distilled water provides a perfect, soft nutrient medium for the organisms.

	(Photos jar)		(Photos jar)

	Next, we needed to sterilize everything inside and outside of the jar, so we placed the lids (equipped with cotton wool in the perforation to prevent any mold from getting inside) into the pressure cooker. One of the jars was marked with tape that would change its color, so we would know when the pressure cooker had finished its sterilization process.		Next, we needed to sterilize everything inside and outside of the jar, so we placed the lids (equipped with cotton wool in the perforation to prevent any mold from getting inside) into the pressure cooker. One of the jars was marked with tape that would change its color, so we would know when the pressure cooker had finished its sterilization process.

	(photos tape, jars in cooker)		(photos tape, jars in cooker)

	After the jars were finished, we noticed the first changes. Due to the heat, the agar agar had turned from a liquid to a solid form. Now, we were able to inoculate our nutrient medium with the mushrooms.		After the jars were finished, we noticed the first changes. Due to the heat, the agar agar had turned from a liquid to a solid form. Now, we were able to inoculate our nutrient medium with the mushrooms.

	( photos)		( photos)

	For this, we used a suction device. After disinfecting all surfaces and preparing everything, we were ready to start. The following materials were needed: disinfectant, gloves, a Bunsen burner, a scalpel, the mushroom cultures, and our jars.		For this, we used a suction device. After disinfecting all surfaces and preparing everything, we were ready to start. The following materials were needed: disinfectant, gloves, a Bunsen burner, a scalpel, the mushroom cultures, and our jars.

Leandra Schieder: /* Day 1: (6h) */

2023-04-19T08:48:02Z

Day 1: (6h)

@@ Line 18: / Line 18: @@
 *Learn to cultivate a mushroom in vitro
 *Document the experiment
 ===Day 2: (6h)===

Miga at 14:10, 11 February 2023

2023-02-11T14:10:23Z

← Older revision		Revision as of 14:10, 11 February 2023
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	''Lecturer(s):'' [[:Category:Alessandro Volpato]]<br />		''Lecturer(s):'' [[:Category:Alessandro Volpato]]<br />
	''Credits:'' 4 [[SWS]]<br />		''Credits:'' 4 [[SWS]]<br />
	Date: ~~4 und 5~~ April<br />		Date: 17 and 19 April<br />
	''Venue:'' DIY Biolab @ [[Marienstraße 7b]], [[Marienstraße 7b/202\|Room 202]]<br />		''Venue:'' DIY Biolab @ [[Marienstraße 7b]], [[Marienstraße 7b/202\|Room 202]]<br />
	Time: 10.00 to 17.00		Time: 17 April 10.00 to 17.00, 19 April 13.30 to 16.45

Miga: /* Project Days: (10h) */

2023-02-02T09:53:48Z

Project Days: (10h)

← Older revision		Revision as of 09:53, 2 February 2023
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	[[Category:~~WS22~~]]		[[Category:SS23]]
	[[Category:Werkmodul]]		[[Category:Werkmodul]]
	[[Category:Fachmodul]]		[[Category:Fachmodul]]
	[[Category:Alessandro Volpato]]		[[Category:Alessandro Volpato]]

Miga at 09:53, 2 February 2023

2023-02-02T09:53:28Z

← Older revision		Revision as of 09:53, 2 February 2023
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	Alessandro Volpato<br>		[[:Category:Werkmodul\|Werkmodul]] [[:Category:Fachmodul\|Fachmodul]]<br />
	~~Termin der ersten Veranstaltung~~ 4 und 5 April<br>		''Lecturer(s):'' [[:Category:Alessandro Volpato]]<br />
	Time: 10.00 ~~bis~~ 17.00		''Credits:'' 4 [[SWS]]<br />
			Date: 4 und 5 April<br />
			''Venue:'' DIY Biolab @ [[Marienstraße 7b]], [[Marienstraße 7b/202\|Room 202]]<br />
			Time: 10.00 to 17.00


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	*Create a small project 7h		*Create a small project 7h
	*Document a small project 1h		*Document a small project 1h


			[[Category:WS22]]
			[[Category:Werkmodul]]
			[[Category:Fachmodul]]
			[[Category:Alessandro Volpato]]

Miga at 14:00, 1 February 2023

2023-02-01T14:00:32Z

← Older revision		Revision as of 14:00, 1 February 2023
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	Alessandro Volpato<br>		Alessandro Volpato<br>
	Termin der ersten Veranstaltung 4 und 5 April,		Termin der ersten Veranstaltung 4 und 5 April<br>
	Time: 10.00 bis 17.00		Time: 10.00 bis 17.00

Miga at 14:00, 1 February 2023

2023-02-01T14:00:21Z

← Older revision		Revision as of 14:00, 1 February 2023
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			Alessandro Volpato<br>
			Termin der ersten Veranstaltung 4 und 5 April,
			Time: 10.00 bis 17.00


	The course has the purpose of introducing the students to the lab, its activities and its equipment. By attending this course students get an allowance to access the biolab.		The course has the purpose of introducing the students to the lab, its activities and its equipment. By attending this course students get an allowance to access the biolab.
	Students will be sollicited to conceptualise own projects and execute it, to get familiar with a biology laboratory routine.		Students will be sollicited to conceptualise own projects and execute it, to get familiar with a biology laboratory routine.

Miga: Created page with "The course has the purpose of introducing the students to the lab, its activities and its equipment. By attending this course students get an allowance to access the biolab. S..."

2023-01-31T17:34:14Z

Created page with "The course has the purpose of introducing the students to the lab, its activities and its equipment. By attending this course students get an allowance to access the biolab. S..."

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The course has the purpose of introducing the students to the lab, its activities and its equipment. By attending this course students get an allowance to access the biolab.
Students will be sollicited to conceptualise own projects and execute it, to get familiar with a biology laboratory routine.

==Syllabus==
===Day 1: (6h)===
*Introduction to the course
*Inputs: Examples of work done in DIY BioLabs

*Introduction to the lab, Lab safety
*Learn to cultivate a mushroom in vitro
*Document the experiment

===Day 2: (6h)===
*Brainstorming session
*Inputs from the lecturer: How to generate ideas in a DIY BioLab
*Generate ideas
*Pitch ideas
*Choose one idea and develop it further

===Project Days: (10h)===

*Conceptualise a small project 2h
*Create a small project 7h
*Document a small project 1h