GMU:DIY Biolab License/Lea-Marie Weigelt - Revision history

Miga: /* Project: Euglena */

2023-02-06T18:27:35Z

Project: Euglena

← Older revision		Revision as of 18:27, 6 February 2023
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	== Project: Euglena==		== Project: Euglena==

	[[File:Konsultation and my own ideas_230117_113402_1.jpg~~\|400px]]~~		<gallery>
			File:Konsultation and my own ideas_230117_113402_1.jpg
	[[File:Konsultation and my own ideas_230117_113402_2.jpg~~\|400px]]~~		File:Konsultation and my own ideas_230117_113402_2.jpg
			File:Konsultation and my own ideas_230117_113402_3.jpg
	[[File:Konsultation and my own ideas_230117_113402_3.jpg~~\|400px]]~~		File:Konsultation and my own ideas_230117_113402_4.jpg
			File:Konsultation and my own ideas_230117_113402_5.jpg
	[[File:Konsultation and my own ideas_230117_113402_4.jpg~~\|400px]]~~		</gallery>

	[[File:Konsultation and my own ideas_230117_113402_5.jpg~~\|400px]]~~


	=== To-Do's ===		=== To-Do's ===

Miga at 18:26, 6 February 2023

2023-02-06T18:26:33Z

Show changes

Miga at 18:23, 6 February 2023

2023-02-06T18:23:41Z

← Older revision		Revision as of 18:23, 6 February 2023
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	* examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases		* examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases

	== ~~Chosen Organism~~: Euglena ==		== Project: Euglena==

			=== To-Do's ===
	== To-Do's ==
	*grow Euglena		*grow Euglena
	* care for Euglena		* care for Euglena
	* work out a creative Projekt using Euglena		* work out a creative Projekt using Euglena

	== Process of growing Euglena: ==		=== Process of growing Euglena: ===
	I met with Alessandro on the 16th of November to start growing Euglena gracilis.		I met with Alessandro on the 16th of November to start growing Euglena gracilis.

	== Making the substrate ==		=== Making the substrate ===
	For the substrate we followed this guide.		For the substrate we followed this guide.

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	I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena.		I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena.

	I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products.		I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products.

	I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker.		I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker.

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	</gallery>		</gallery>

	== Ideas for using Euglena ==		=== Ideas for using Euglena ===

	[[File:Konsultation and my own ideas_230117_113402_1.jpg\|400px]]		[[File:Konsultation and my own ideas_230117_113402_1.jpg\|400px]]
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	[[File:Konsultation and my own ideas_230117_113402_5.jpg\|400px]]		[[File:Konsultation and my own ideas_230117_113402_5.jpg\|400px]]

	== Footage of the grown Euglena ==		=== Footage of the grown Euglena ===

	<gallery>		<gallery>
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	<videoflash type="vimeo">795559259\|437\|236</videoflash>		<videoflash type="vimeo">795559259\|437\|236</videoflash>

	== Problems/Difficulties that occured during the process ==		=== Problems/Difficulties that occured during the process ===

	The first thing that happened that didn't turn out to be too big of a deal was that only one of the four tubes with Euglena that we made was actually usable. The others had contaminations and/or didn't grow. Which is a good reminder that these things can happen when you are working with living beings. So always have a backup and check in regularly to make sure they're still thriving.		The first thing that happened that didn't turn out to be too big of a deal was that only one of the four tubes with Euglena that we made was actually usable. The others had contaminations and/or didn't grow. Which is a good reminder that these things can happen when you are working with living beings. So always have a backup and check in regularly to make sure they're still thriving.

Miga: /* To-Do's */

2023-02-06T18:21:12Z

To-Do's

← Older revision		Revision as of 18:21, 6 February 2023
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	== To-Do's ==		== To-Do's ==
			*grow Euglena
	- grow Euglena		* care for Euglena
			* work out a creative Projekt using Euglena
	- care for Euglena

	- work out a creative Projekt using Euglena

	== Process of growing Euglena: ==		== Process of growing Euglena: ==

Miga: /* 05.11.22 10 am to 2 pm */

2023-02-06T18:20:40Z

05.11.22 10 am to 2 pm

← Older revision		Revision as of 18:20, 6 February 2023
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	== 05.11.22 10 am to 2 pm==		== 05.11.22 10 am to 2 pm==

	Plan: Projectdraft		Plan: Projectdraft

	Presentation of some organisms		Presentation of some organisms

	-> Brainstorming to one organism in groups of 3-4 people (what can we do with it? how can we present that idea?) and create 5 ideas		===Brainstorming to one organism in groups of 3-4 people ===
			*what can we do with it?
			* how can we present that idea?
			* and create 5 ideas


	Creatures:		Creatures:

	slime mold: one big cell that grows toeards the food. What experiments can be done with it? Idea: how would slime mold plan rail networks		*slime mold: one big cell that grows toeards the food. What experiments can be done with it? Idea: how would slime mold plan rail networks
			*Euglena (Algae): can photosynthesize and reuse CO2 ; moves slowly towards the light
	Euglena (Algae): can photosynthesize and reuse CO2 ; moves slowly towards the light		*Mycelium: Idea: modular bricks for building structures
			*Glow in the dark mushrooms (pomellus stipticus): idea: exitlights/nightlights filled with mushrooms
	Mycelium: Idea: modular bricks for building structures		*Kombucha (yeast and bacteria living together) creating paperlike structure when dried

	Glow in the dark mushrooms (pomellus stipticus): idea: exitlights/nightlights filled with mushrooms

	~~Combucha (yeast and bacteria living together) creating paperlike structure when dried~~

	One hour to make up 5 ideas		One hour to make up 5 ideas
	-> Why? How? What?		* Why? How? What?

	1. Exit signs from glowing mushrooms (brightness?lifetime?)		1. Exit signs from glowing mushrooms (brightness?lifetime?)
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	what? compostable pen cases; maybe Diy Kits for teens		what? compostable pen cases; maybe Diy Kits for teens

	~~- 20 Minute break -~~

	Group session 2		===Group session 2===
			* Develop one of our ides further; chosen idea: glowing exit signs

	~~-> Develop one of our ides further; chosen idea: glowing exit signs~~

	structure:		structure:

	why?		why?
			* saving electricity
			* sustainability

	~~-> saving electricity~~

	~~-> sustainability~~

	realisation process		realisation process
			* grow fungi (panellus stypticus)
			* inside glass cube
			* run some tests on when the mushroom glows
			* setup gives mushroom food and moisture
			* find out more about mushrooms biorythm

	~~-> grow fungi (panellus stypticus)~~

	~~-> inside glass cube~~

	~~-> run some tests on when the mushroom glows~~

	~~-> setup gives mushroom food and moisture~~

	~~-> find out more about mushrooms biorythm~~

	Plans for setup		Plans for setup
			* reuse old exit-sign cases (test if it works with the plastic and color)
			* fill it with substrate and grow mushroom in there
			* install mushroom case

	~~-> reuse old exit-sign cases (test if it works with the plastic and color)~~

	~~-> fill it with substrate and grow mushroom in there~~

	~~-> install mushroom case~~

	where do we want to exhibit it? Showcase 3 places here in Weimar		where do we want to exhibit it? Showcase 3 places here in Weimar
			* University
			* Lichthaus Kino
			* Jena botanischer Garten

	~~-> University~~

	~~-> Lichthaus Kino~~

	~~-> Jena botanischer Garten~~

	Necessary tests:		Necessary tests:
			* biorythm testing
	-> biorythm testing		* longtime study of mushroom lifespan
			* temperature? Is roomtemperature okay?
	-> longtime study of mushroom lifespan		* Nutrition? Is moisture in the substrate?
			* when does it glow? How does it decide to glow? Can it be used during the day?
	-> temperature? Is roomtemperature okay?		* examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases

	-> Nutrition? Is moisture in the substrate?

	-> when does it glow? How does it decide to glow? Can it be used during the day?

	-> examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases

	== Chosen Organism: Euglena ==		== Chosen Organism: Euglena ==

Miga: /* Ethics and values in the lab */

2023-02-06T18:17:03Z

Ethics and values in the lab

← Older revision		Revision as of 18:17, 6 February 2023
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	== Ethics and values in the lab ==		== Ethics and values in the lab ==
			* respect for our creatures
			* respect for lab partners
			* don't take criticism personally
			* only go to the lab when you are healthy and in the right mood (concentrated; healthy; good mindful mindstate)
			* respect time and projects of others (name everything; report when things run out or get broken)

	~~-> respect for our creatures~~

	~~-> respect for lab partners~~

	~~-> don't take criticism personally~~

	~~-> only go to the lab when you are healthy and in the right mood (concentrated; healthy; good mindful mindstate)~~

	~~-> respect time and projects of others (name everything; report when things run out or get broken)~~

	How to respect our creatures and each other:		How to respect our creatures and each other:
			* what is the specimen? (mushroom parts still thrive even if you take parts of it for example)
	-> what is the specimen? (mushroom parts still thrive even if you take parts of it for example)		* destroy things that aren't from here
			* nothing leaves the lab alive unless it comes from the natural habitat outside
	-> destroy things that aren't from here		* we work to help understand more for everyone (goal: sustainability)
			* respect experts from other fields (everybody can provide value)
	-> nothing leaves the lab alive unless it comes from the natural habitat outside		* be inclusive; everybody is equal

	-> we work to help understand more for everyone (goal: sustainability)

	-> respect experts from other fields (everybody can provide value)

	-> be inclusive; everybody is equal

	== 05.11.22 10 am to 2 pm==		== 05.11.22 10 am to 2 pm==

Miga: /* 3. Knowledge */

2023-02-06T18:16:22Z

3. Knowledge

← Older revision		Revision as of 18:16, 6 February 2023
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	*Radical Mycology: A Treatise on Seeing & working with Fungi (2016)		*Radical Mycology: A Treatise on Seeing & working with Fungi (2016)
	*The fifth kingdom; An introduction to mycology Bryce Kendrick (2017)		*The fifth kingdom; An introduction to mycology Bryce Kendrick (2017)


	Academical knowledge		Academical knowledge
	*first -> wikipedia and sources (gives good overview; sources; scientific papers)		*first -> wikipedia and sources (gives good overview; sources; scientific papers)
	*second -> academic papers (google schola; unpaywall.org; if behind paywall: ask for copy; wikipedia.org/Sci-Hub: grey area of copy right)		*second -> academic papers (google schola; unpaywall.org; if behind paywall: ask for copy; wikipedia.org/Sci-Hub: grey area of copy right)


	"if you have a big goal break it up into small goals you can achieve"		"if you have a big goal break it up into small goals you can achieve"

Miga: /* Growing our own mushrooms */

2023-02-06T18:16:06Z

Growing our own mushrooms

← Older revision		Revision as of 18:16, 6 February 2023
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	what do we need?		what do we need?

	1. Mushroom/spores		===1. Mushroom/spores===
	where can you find it:		where can you find it:

	online shops		online shops
	-> Pilzmännchen.de		* Pilzmännchen.de
	-> MycoGenetics		* MycoGenetics

	the forest:		the forest:
	-> on the ground		* on the ground
	-> on dead logs or living trees		* on dead logs or living trees

	~~2. Substrates (mushroom food):~~

			===2. Substrates (mushroom food): ===
	buy at local stores or online		buy at local stores or online
	-> Bricolage market (saw dust)		* Bricolage market (saw dust)
	-> Pet shop		* Pet shop
	-> Carpenter		* Carpenter

	~~3. Knowledge~~

			===3. Knowledge===
	Books:		Books:

	Mycelium running: how mushrooms can help save the world (2004)		*Mycelium running: how mushrooms can help save the world (2004)
			*Organic mushroom farming and mycoremediation (2014)
	Organic mushroom farming and mycoremediation (2014)		*Radical Mycology: A Treatise on Seeing & working with Fungi (2016)
			*The fifth kingdom; An introduction to mycology Bryce Kendrick (2017)
	Radical Mycology: A Treatise on Seeing & working with Fungi (2016)

	The fifth kingdom; An introduction to mycology Bryce Kendrick (2017)

	Academical knowledge		Academical knowledge
			*first -> wikipedia and sources (gives good overview; sources; scientific papers)
	first -> wikipedia and sources (gives good overview; sources; scientific papers)		*second -> academic papers (google schola; unpaywall.org; if behind paywall: ask for copy; wikipedia.org/Sci-Hub: grey area of copy right)

	second -> academic papers (google schola; unpaywall.org; if behind paywall: ask for copy; wikipedia.org/Sci-Hub: grey area of copy right)

	"if you have a big goal break it up into small goals you can achieve"		"if you have a big goal break it up into small goals you can achieve"

Miga: /* Building a Diy-Lab at home */

2023-02-06T18:14:06Z

Building a Diy-Lab at home

@@ Line 30: / Line 30: @@
 Equipment:
-local shopping
+=== local shopping ===
--> hardware store (tables with a glass or ceramic top)
+===online shopping===
--> bricolage market
+===consumables (cottonswabs/gloves etc.)===
--> artists shop
+===chemicals===
+* Buying at local stores (check cross-application; check purity grade according to use)
--> pharmacies
+* online shopping (check if law allows private purchase)
+* maybe extract your chemicals from mixtures (know what you are doing and with whom)
 == Growing our own mushrooms ==

Miga: /* Documenation of the main Workshop 04.11.22 */

2023-02-06T18:12:39Z

Documenation of the main Workshop 04.11.22

← Older revision		Revision as of 18:12, 6 February 2023
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	Plan for today (04.11) and tomorrow (05.11):		Plan for today (04.11) and tomorrow (05.11):

	-> cultivate first organism (mushroom)		* cultivate first organism (mushroom)
			* lab safety (how to work safely in the lab)
	-> lab safety (how to work safely in the lab)		* getting to know the lab equipment
			* Redesign the lab experience for our own home (How to make our own Diy-Biolab)
	-> getting to know the lab equipment		* lab ethics and values

	-> Redesign the lab experience for our own home (How to make our own Diy-Biolab)

	-> lab ethics and values

	Materials and manual for growing first organisms:		Materials and manual for growing first organisms:
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	examples:		examples:

	-> Algae can photosynthesize; they need light and salt		* Algae can photosynthesize; they need light and salt
			* slime mold (unicellar organisms that expand in search of food; they're neither fungi nor mushrooms)
	-> slime mold (unicellar organisms that expand in search of food; they're neither fungi nor mushrooms)		* bacteria

	-> bacteria

	~~- We had a break from 11:45 am to 01:30 pm -~~

	== Building a Diy-Lab at home ==		== Building a Diy-Lab at home ==