GMU:DIY Biolab “Driver’s License”/DNA extraction - Revision history

Miga: /* DNA */

2017-11-01T15:40:12Z

DNA

← Older revision		Revision as of 15:40, 1 November 2017
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	<gallery>		<gallery>
			File:Wilson1900Fig2.jpg \|Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia)
	File:Celltypes.png \|A eukaryotic cell (left) and a prokaryotic cell (right) (wikipedia)		File:Celltypes.png \|A eukaryotic cell (left) and a prokaryotic cell (right) (wikipedia)
	~~File:Wilson1900Fig2.jpg \|Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia)~~
	File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia)		File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia)
	File:1-dna-polymerase.jpg		File:1-dna-polymerase.jpg

Miga: /* DNA */

2017-11-01T15:39:54Z

DNA

← Older revision		Revision as of 15:39, 1 November 2017
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	<gallery>		<gallery>
			File:Celltypes.png \|A eukaryotic cell (left) and a prokaryotic cell (right) (wikipedia)
	File:Wilson1900Fig2.jpg \|Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia)		File:Wilson1900Fig2.jpg \|Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia)
	File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia)		File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia)

Miga: /* DNA */

2017-11-01T15:38:11Z

DNA

← Older revision		Revision as of 15:38, 1 November 2017
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	<gallery>		<gallery>
	File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.		File:Wilson1900Fig2.jpg \|Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia)
			File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia)
	File:1-dna-polymerase.jpg		File:1-dna-polymerase.jpg
	File:2-dna-structure.jpg		File:2-dna-structure.jpg

Miga: /* DNA */

2017-11-01T15:35:56Z

DNA

← Older revision		Revision as of 15:35, 1 November 2017
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	<gallery>		<gallery>
	File:DNA_Structure+Key+Labelled.pn_NoBB.png The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.		File:DNA_Structure+Key+Labelled.pn_NoBB.png \|The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.
	File:1-dna-polymerase.jpg		File:1-dna-polymerase.jpg
	File:2-dna-structure.jpg		File:2-dna-structure.jpg

Miga: /* DNA */

2017-11-01T15:35:31Z

DNA

← Older revision		Revision as of 15:35, 1 November 2017
Line 91:		Line 91:

	<gallery>		<gallery>
			File:DNA_Structure+Key+Labelled.pn_NoBB.png The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.
	File:1-dna-polymerase.jpg		File:1-dna-polymerase.jpg
	File:2-dna-structure.jpg		File:2-dna-structure.jpg

Miga: /* DNA extraction */

2017-11-01T15:30:39Z

DNA extraction

← Older revision		Revision as of 15:30, 1 November 2017
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	==DNA extraction==		==DNA extraction==
			https://www.youtube.com/watch?v=7wU97pTs-xE

	===Meat tissue cells===		===Meat tissue cells===
	*mince thoroughly a small piece of meat tissue		*mince thoroughly a small piece of meat tissue

Miga: /* DNA */

2017-11-01T14:42:35Z

DNA

← Older revision		Revision as of 14:42, 1 November 2017
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	“The double-helix model of DNA structure was first published in the journal Nature by James Watson and Francis Crick in 1953”(https://en.wikipedia.org/wiki/Nucleic_acid_double_helix)		“The double-helix model of DNA structure was first published in the journal Nature by James Watson and Francis Crick in 1953”(https://en.wikipedia.org/wiki/Nucleic_acid_double_helix)

			<gallery>
			File:1-dna-polymerase.jpg
			File:2-dna-structure.jpg
			File:3-what.jpg
			File:4-how.jpg
			</gallery>

	===PCR===		===PCR===

Miga: /* Mouth cells */

2017-11-01T11:08:50Z

Mouth cells

← Older revision		Revision as of 11:08, 1 November 2017
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	===Mouth cells===		===Mouth cells===
	*50ml Falkon mit 50ml isotonischer Kochsalzlösung		*50ml Falkon mit 50ml [https://en.wikipedia.org/wiki/Saline_(medicine) isotonischer Kochsalzlösung]
	*damit den Mund ordentlich ausspülen (1-2min)		*damit den Mund ordentlich ausspülen (1-2min)
	*zurück in den Falkon spucken		*zurück in den Falkon spucken

Miga: /* Joe Davis, Microvenus* */

2017-10-31T19:44:18Z

Joe Davis, Microvenus*

← Older revision		Revision as of 19:44, 31 October 2017
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	*http://www.artscatalyst.org/sterile-sensei-ichi-go		*http://www.artscatalyst.org/sterile-sensei-ichi-go

	===Joe Davis, Microvenus*===		===Joe Davis, Microvenus===
	[[File:microvenus-unmondemoderne.wordpress.jpg\|thumb\|Fig. 6. Microvenus. Source: http://unmondemoderne.wordpress.com ]]		[[File:microvenus-unmondemoderne.wordpress.jpg\|thumb\|Fig. 6. Microvenus. Source: http://unmondemoderne.wordpress.com ]]
	Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow:		Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow:

	"Each Microvenus organism contains many copies of a special molecule designed by the artist and his colleagues. The artistic molecule is a short piece of synthetic DNA containing a coded visual icon that has been incorporated into a living strain of bacteria (E. Coli)." ~~(Davis 1996).~~		"Each Microvenus organism contains many copies of a special molecule designed by the artist and his colleagues. The artistic molecule is a short piece of synthetic DNA containing a coded visual icon that has been incorporated into a living strain of bacteria (E. Coli)."*

	The coded icon is a symbol of life from a germanic rune. Resembling letter Y it also represents a female earth in different mythologies. The graphical image was converted into the bit-map image and further into DNA base pairs in order to synthetically program and attach to the original DNA code of the plasmids carried by bacteria (Fig. 6.). Davis has imagined that the code might be interpreted by extraterrestrials as the final stage of the project was supposed to include shipping of the modified bacteria into the universe.		The coded icon is a symbol of life from a germanic rune. Resembling letter Y it also represents a female earth in different mythologies. The graphical image was converted into the bit-map image and further into DNA base pairs in order to synthetically program and attach to the original DNA code of the plasmids carried by bacteria (Fig. 6.). Davis has imagined that the code might be interpreted by extraterrestrials as the final stage of the project was supposed to include shipping of the modified bacteria into the universe.

Miga: /* Joe Davis, Microvenus */

2017-10-31T19:43:48Z

Joe Davis, Microvenus

← Older revision		Revision as of 19:43, 31 October 2017
Line 57:		Line 57:
	*http://www.artscatalyst.org/sterile-sensei-ichi-go		*http://www.artscatalyst.org/sterile-sensei-ichi-go

	===Joe Davis, Microvenus===		===Joe Davis, Microvenus*===
	[[File:microvenus-unmondemoderne.wordpress.jpg\|thumb\|Fig. 6. Microvenus. Source: http://unmondemoderne.wordpress.com ]]		[[File:microvenus-unmondemoderne.wordpress.jpg\|thumb\|Fig. 6. Microvenus. Source: http://unmondemoderne.wordpress.com ]]
	Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow:		Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow: