GMU:DIY-Microscopy/Jakob - Revision history

Max: /* Tracking Blobs */

2013-05-16T17:35:28Z

Tracking Blobs

← Older revision		Revision as of 17:35, 16 May 2013
Line 135:		Line 135:
	==== Tracking Blobs ====		==== Tracking Blobs ====

	Currently, I am working on a [~~http://puredata.info/\|~~ Pure Data] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.		Currently, I am working on a [[Pure Data]] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.

Zoro at 21:32, 31 March 2013

2013-03-31T21:32:47Z

← Older revision		Revision as of 21:32, 31 March 2013
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	Currently, I am working on a [http://puredata.info/\| Pure Data] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.		Currently, I am working on a [http://puredata.info/\| Pure Data] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.
	~~/videoflash>~~

	~~== First artistical tries ==~~
	~~[[File:Microscope-jakob-drying-condiments.jpg\|195px\|right\|thumb\|Condiments drying at a radiator]]~~

	In her talk about the basics of microscopy, Adriana showed us some nice images captured with microscopes such as the ones on [http://scienceblogs.com/retrospectacle/2007/01/30/beer-microscopy-project/ this site]. Since I liked the way the artists colored their specimen, I asked myself how to achieve a similar effect with possible cheap equipment.

	~~So, I considered to dye sugar, salt and meal with food coloring and then take gaudy images of it. In the photo to the right, one can see the condiments drying at a radiator after coloring.~~

	~~Here is a gallery of some images I shot:~~

Zoro at 17:57, 31 March 2013

2013-03-31T17:57:23Z

← Older revision		Revision as of 17:57, 31 March 2013
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	\|}		\|}

	A M4 thread has a pitch of 0.7 mm, which means that one rotation about its own axis will move the lower sled 0.7 mm and consequently the upper sled about 0.2 mm. As a result, the microscope can be adjusted very fine in its height.		A M4 thread has a pitch of 0.7 mm, which means that one rotation about its own axis will move the lower sled 0.7 mm and consequently the upper sled about 0.2 mm. As a result, the microscope can be adjusted very fine in its height. However, compared to the cases built by my fellow students (see [[GMU:DIY-Microscopy#Works\|Works]]), my approach is quite complicated and a bit more expensive just to fulfil the same functionality.

	''Note:'' Unfortunately the 3D printer became damaged; so, the case itself is built out of Balsa wood and could not be printed during this course. The sleds were printed before the printer fell off the table.		''Note:'' Unfortunately the 3D printer became damaged; so, the case itself is built out of Balsa wood and could not be printed during this course. The sleds were printed before the printer fell off the table.
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	Currently, I am working on a [http://puredata.info/\| Pure Data] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.		Currently, I am working on a [http://puredata.info/\| Pure Data] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.
			/videoflash>

			== First artistical tries ==
			[[File:Microscope-jakob-drying-condiments.jpg\|195px\|right\|thumb\|Condiments drying at a radiator]]

			In her talk about the basics of microscopy, Adriana showed us some nice images captured with microscopes such as the ones on [http://scienceblogs.com/retrospectacle/2007/01/30/beer-microscopy-project/ this site]. Since I liked the way the artists colored their specimen, I asked myself how to achieve a similar effect with possible cheap equipment.

			So, I considered to dye sugar, salt and meal with food coloring and then take gaudy images of it. In the photo to the right, one can see the condiments drying at a radiator after coloring.

			Here is a gallery of some images I shot:

Zoro at 17:47, 31 March 2013

2013-03-31T17:47:03Z

← Older revision		Revision as of 17:47, 31 March 2013
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	<videoflash type="vimeo">55721018\|628\|480</videoflash>		<videoflash type="vimeo">55721018\|628\|480</videoflash>

			== More Approaches ==

			After having built the microscope and knowing its strengths and weaknesses, I started to think about more things to with this and other microscopes. Here are some ideas I wanted to follow:

			==== 3D microscopy ====

			Basically, there are three methods for getting 3D images of your specimen:

			* having a special lens so that the camera is able to look at the specimen from multiple view points at only one certain point in time ([http://phys.org/news/2011-03-stereo-lens-d-microscope.html\| see this article]),
			* using two cameras to get two images at one certain point in time, or
			* using only one camera and move either the camera or the specimen after capturing the first photo to capture another one at a different point in time.

			The special lens is far too expensive for our little project. For the second method, one would need two very tiny cameras or a mirror system to achieve good results. Since we cannot mount cameras and/or mirrors with such a high precision, this approach did not work either. Same holds for the third one.

			==== Low-Cost Microscopes for Schools ====

			Today almost every teenager owns a smartphone with a camera on board. Max came up with the idea that it might be really interesting to use this camera with a special mounted lens to have a low-cost microscope for school lessons, particularly in biology teaching. With my old smartphone I just tinkered a little around, but could only try very few lenses lying around with really bad results. However, this idea should definitely be followed in the future with more knowledge about complex lens systems.

			==== Making Electricity Visible ====

			This one is quite easy: I wanted to create a spark and view it under the microscope. One could even try to melt sugar to caramel, or burn a piece of paper and record a slow motion video of this process. Unfortunately, I have not found a battery in an appropriate price range that was strong enough to produce a spark that is visible. Maybe this approach can be followed by someone who has an old car battery standing around.

			==== Tracking Blobs ====

			Currently, I am working on a [http://puredata.info/\| Pure Data] patch to help Max with a blob tracking algorithm. Since I am new to Pd, I just did not finish my work yet, but I will upload the patch as soon as it works.

Zoro at 16:45, 31 March 2013

2013-03-31T16:45:05Z

← Older revision		Revision as of 16:45, 31 March 2013
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	~~''Note:'' You can download [[Media:Microscope-jakob-documentation.pdf\|this documentation as PDF file]].~~

	== Introduction ==		== Introduction ==
	Goal of the workshop was to repurpose a possible cheap USB webcam into a microscope. Max Neupert, the organizer of this workshop, told us how to achieve this and instructed us throughout the winter term 2012/13.		Goal of the workshop was to repurpose a possible cheap USB webcam into a microscope. Max Neupert, the organizer of this workshop, told us how to achieve this and instructed us throughout the winter term 2012/13.
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	== Disassembling the Camera ==		== Disassembling the Camera ==
	The "Hama PC-Webcam AC-150" served as a basis, which costs about € 5 when ordering via Amazon. It has a resolution of 640x480 pixels and six LEDs to lighten the scene. Beyond this, the camera supports UVC (USB Video Class), which is important for viewing live images directly on the screen.		The "Hama PC-Webcam AC-150" served as a basis, which costs about € 5 when ordering via [http://www.amazon.de/Hama-AC-150-Webcam-Megapixel-USB/dp/B001P30EDE/ref=sr_1_1?ie=UTF8&qid=1364744670&sr=8-1\| Amazon]. It has a resolution of 640x480 pixels and six LEDs to lighten the scene. Beyond this, the camera supports UVC (USB Video Class), which is important for viewing live images directly on the screen.

	As mentioned before, the first step was to disassemble the camera. After cracking the case, six LEDs had to be removed. In order to do so, we had to heat the solder at the back side of the PCB (printed circuit board). The last important step was to mount the lens with hot glue upside down onto the PCB. ~~A photo showing this state of~~ the ~~microscope will follow soon~~.		As mentioned before, the first step was to disassemble the camera. After cracking the case, six LEDs had to be removed. In order to do so, we had to heat the solder at the back side of the PCB (printed circuit board). The last important step was to mount the lens with hot glue upside down onto the PCB. If needed, one can solder flex (in German: Litze) with the remounted LEDs back on the PCB for beeing able to lighten the specimen.

	<gallery>		<gallery>
	File:Microscope-jakob-~~camera~~-disassembling-1.jpg\|~~PCB without camera~~ case~~<br/>next to its original package~~		File:Microscope-jakob-disassembling-3.jpg\|Unmounted from stand
	File:Microscope-jakob-~~camera~~-disassembling-2.jpg\|~~PCB~~ with ~~unsoldered<br/>~~LEDs ~~in front~~		File:Microscope-jakob-disassembling-4.jpg\|After cracking case (1)
			File:Microscope-jakob-disassembling-5.jpg\|After cracking case (2)
			File:Microscope-jakob-disassembling-6.jpg\|Unsoldered LEDs
			File:Microscope-jakob-disassembling-7.jpg\|Unmounted lens
			File:Microscope-jakob-disassembling-8.jpg\|Remounted lens
			File:Microscope-jakob-disassembling-9.jpg\|Flex with LEDs
	</gallery>		</gallery>

	The resulting microscope has a magnification area of 0.~~35mm~~ x 0.~~46mm~~. This can be calculated by counting the visible pixels of a display with known ppi-specification. In my case the microscope shows 3x4 pixels with a length of 0.~~117mm~~ per pixel (HTC HD2 has 217 ppi).		The resulting microscope has a magnification area of 0.35 mm x 0.46 mm. This can be calculated by counting the visible pixels of a display with known ppi-specification. In my case the microscope shows 3x4 pixels with a length of 0.117 mm per pixel (HTC HD2 has 217 ppi).

	== Building a Case ==		== Building a Case ==
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	[[File:Microscope-case-jakob-rendered.jpg\|195px\|thumb\|right\|Rendered image of first draft]]		[[File:Microscope-case-jakob-rendered.jpg\|195px\|thumb\|right\|Rendered image of first draft]]

	Since a microscope is designed to show very small objects, it is not appropriate to hold the camera in the hand because of uncontrollable hand movements. Therefore we needed to build a case that holds the microscope and gives the possibility to do little adjustments in height (~~that’s~~ our zoom).		Since a microscope is designed to show very small objects, it is not appropriate to hold the camera in the hand because of uncontrollable hand movements. Therefore, we needed to build a case that holds the microscope and gives the possibility to do little adjustments in height (that is our zoom).

	My approach basically consists of two sleds that lie on one another where the contact surface is a plane in the angle of 15 degrees to the table. Pushing the lower carriage to the left will force the upper carriage to move upwards. The first image on the right shows a technical side view drawing, the second one shows a rendered image.		My approach basically consists of two sleds that lie on one another where the contact surface is a plane in the angle of 15 degrees to the table. Pushing the lower carriage to the left will force the upper carriage to move upwards. The first image on the right shows a technical side view drawing, the second one shows a rendered image.
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	[[File:Microscope-jakob-case-sketchup-model.png‎\|195px\|right\|thumb\|SketchUp model of case ([[Media:Microscope-case-jakob-sketchup-model.zip\|Download]])]]		[[File:Microscope-jakob-case-sketchup-model.png‎\|195px\|right\|thumb\|SketchUp model of case ([[Media:Microscope-case-jakob-sketchup-model.zip\|Download]])]]

	After building a prototype out of Balsa wood, I was able to prove that the construction works fine. ~~Nevertheless~~, some minor changes to the model ~~have been done~~ before printing the parts with a [[GMU:RapMan\|RapMan 3D printer]]. In the image below, that shows the two printed sleds carrying the microscope, one can notice that a M4 threaded rod was stuck through the lower sled and two counter nuts were fastened at each side of the sled, whereas the original plan was to have a screw head lying in a little pocket.		After building a prototype out of Balsa wood, I was able to prove that the construction works fine. However, some minor changes have been applied to the model before printing the parts with a [[GMU:RapMan\|RapMan 3D printer]]. In the image below, that shows the two printed sleds carrying the microscope, one can notice that a M4 threaded rod was stuck through the lower sled and two counter nuts were fastened at each side of the sled, whereas the original plan was to have a screw head lying in a little pocket.

	{\|		{\|
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	\|}		\|}

	A M4 thread has a pitch of 0.7 mm, which means that one rotation about its own axis will move the lower sled 0.7 mm and ~~therefore~~ the upper sled about 0.2 mm. As a result, the microscope can be adjusted very fine in its height.		A M4 thread has a pitch of 0.7 mm, which means that one rotation about its own axis will move the lower sled 0.7 mm and consequently the upper sled about 0.2 mm. As a result, the microscope can be adjusted very fine in its height.

	''Note:'' As the 3D printer ~~is broken at the moment~~, the case is built out of Balsa wood. ~~An image of~~ the ~~actual model will be uploaded as soon as possible~~.		''Note:'' Unfortunately the 3D printer became damaged; so, the case itself is built out of Balsa wood and could not be printed during this course. The sleds were printed before the printer fell off the table.

			<gallery>
			File:Microscope-jakob-sleds-upper-position.jpg\|Sleds at upper position
			File:Microscope-jakob-sleds-lower-position.jpg\|Sleds at lower position
			</gallery>

	== WebcamCapture Application ==		== WebcamCapture Application ==
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	== First images ==		== First images ==
	Here are my first images taken with the microscope. Unfortunately, on almost all images one can see a ~~blot~~ (probably hot glue). I ~~will get another camera and try~~ to do better work.		Here are my first images taken with the microscope. Unfortunately, on almost all images one can see a blob (probably hot glue). I decided to do some better work with a totally new camera, but this one produced even worse images since it had a lower resolution and magnification.

	<gallery>		<gallery>
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	</gallery>		</gallery>

	~~And here's~~ a video of moving bubbles in a drop of water of the river Ilm.		Here is a video of moving bubbles in a drop of water of the river Ilm.

	<videoflash type="vimeo">~~54222462~~\|640\|480</videoflash>		<videoflash type="vimeo">62989767\|640\|480</videoflash>

	== First artistical tries ==		== First artistical tries ==
	[[File:Microscope-jakob-drying-condiments.jpg\|195px\|right\|thumb\|Condiments drying at a radiator)]]		[[File:Microscope-jakob-drying-condiments.jpg\|195px\|right\|thumb\|Condiments drying at a radiator]]

	In her talk about the basics of microscopy, Adriana showed us some nice images captured with microscopes, such as the ones on [http://scienceblogs.com/retrospectacle/2007/01/30/beer-microscopy-project/ this site]. Since I liked the way the artists colored their specimen, I asked myself how to achieve a similar effect with possible cheap equipment.		In her talk about the basics of microscopy, Adriana showed us some nice images captured with microscopes such as the ones on [http://scienceblogs.com/retrospectacle/2007/01/30/beer-microscopy-project/ this site]. Since I liked the way the artists colored their specimen, I asked myself how to achieve a similar effect with possible cheap equipment.

	So, I considered to dye sugar, salt and meal with food coloring and then take gaudy images of it. In the photo to the right, one can see the ~~three~~ condiments drying at a radiator after coloring.		So, I considered to dye sugar, salt and meal with food coloring and then take gaudy images of it. In the photo to the right, one can see the condiments drying at a radiator after coloring.

	~~And here~~ is a gallery of some images I shot:		Here is a gallery of some images I shot:

	<gallery perrow="4">		<gallery perrow="4">
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	</gallery>		</gallery>

	Some of the images are nice-looking, but after all I'm not satisfied with the results. One big problem I noticed when taking these photos is that the camera has an automatic white balance; so, it was the best to always keep the Petri dish in motion, which made it quite hard to ~~shoot~~ good photos.		Some of the images are nice-looking, but after all I am not satisfied with the results. One big problem I noticed when taking these photos, is that the camera has an automatic white balance; so, it was the best to always keep the Petri dish in motion, which made it quite hard to capture good photos.

	~~However, I will look for other exciting effects that can be achieved with our self-made microscope~~.

	== Microscopic World: Waterdrops ==		== Microscopic World: Waterdrops ==

	~~Here's a video~~ I ~~took of salt and sugar giving~~ a drop of water onto ~~them~~.		When playing around with the colored condiments, I wondered how it would look like when one gives a drop of water onto salt and sugar. Here is the result:

	<videoflash type="vimeo">55721018\|628\|480</videoflash>		<videoflash type="vimeo">55721018\|628\|480</videoflash>

Zoro: /* Microscopic World: Waterdrops */

2012-12-17T06:44:46Z

Microscopic World: Waterdrops

← Older revision		Revision as of 06:44, 17 December 2012
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	== Microscopic World: Waterdrops ==		== Microscopic World: Waterdrops ==

	Here's a video I took of salt and sugar ~~respectively~~ giving a drop of water on them.		Here's a video I took of salt and sugar giving a drop of water onto them.

	<videoflash type="vimeo">55721018\|~~640~~\|480</videoflash>		<videoflash type="vimeo">55721018\|628\|480</videoflash>

Zoro at 20:02, 16 December 2012

2012-12-16T20:02:25Z

← Older revision		Revision as of 20:02, 16 December 2012
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	However, I will look for other exciting effects that can be achieved with our self-made microscope.		However, I will look for other exciting effects that can be achieved with our self-made microscope.

			== Microscopic World: Waterdrops ==

			Here's a video I took of salt and sugar respectively giving a drop of water on them.

			<videoflash type="vimeo">55721018\|640\|480</videoflash>

Zoro at 20:21, 3 December 2012

2012-12-03T20:21:58Z

← Older revision		Revision as of 20:21, 3 December 2012
Line 70:		Line 70:

	<videoflash type="vimeo">54222462\|640\|480</videoflash>		<videoflash type="vimeo">54222462\|640\|480</videoflash>

			== First artistical tries ==
			[[File:Microscope-jakob-drying-condiments.jpg\|195px\|right\|thumb\|Condiments drying at a radiator)]]

			In her talk about the basics of microscopy, Adriana showed us some nice images captured with microscopes, such as the ones on [http://scienceblogs.com/retrospectacle/2007/01/30/beer-microscopy-project/ this site]. Since I liked the way the artists colored their specimen, I asked myself how to achieve a similar effect with possible cheap equipment.

			So, I considered to dye sugar, salt and meal with food coloring and then take gaudy images of it. In the photo to the right, one can see the three condiments drying at a radiator after coloring.

			And here is a gallery of some images I shot:

			<gallery perrow="4">
			File:Microscope-jakob-plain-sugar-1.png\|Usual sugar
			File:Microscope-jakob-blue-sugar-1.png\|Blue colored sugar
			File:Microscope-jakob-red-sugar-1.png\|Red colored sugar
			File:Microscope-jakob-gaudy-sugar-1.png\|Sugar in multiple colors
			File:Microscope-jakob-usual-salt-1.png\|Usual salt
			File:Microscope-jakob-blue-salt-1.png\|Blue colored salt
			File:Microscope-jakob-yellow-salt-1.png\|Yellow colored salt
			File:Microscope-jakob-gaudy-salt-1.png\|Salt in multiple colors
			File:Microscope-jakob-usual-meal-1.png\|Usual meal
			File:Microscope-jakob-green-meal-1.png\|Green colored meal
			File:Microscope-jakob-red-meal-1.png\|Red colored meal
			File:Microscope-jakob-gaudy-meal-1.png\|Meal in multiple colors
			</gallery>

			Some of the images are nice-looking, but after all I'm not satisfied with the results. One big problem I noticed when taking these photos is that the camera has an automatic white balance; so, it was the best to always keep the Petri dish in motion, which made it quite hard to shoot good photos.

			However, I will look for other exciting effects that can be achieved with our self-made microscope.

Max at 17:42, 25 November 2012

2012-11-25T17:42:29Z

← Older revision		Revision as of 17:42, 25 November 2012
Line 2:		Line 2:

	== Introduction ==		== Introduction ==

	Goal of the workshop was to repurpose a possible cheap USB webcam into a microscope. Max Neupert, the organizer of this workshop, told us how to achieve this and instructed us throughout the winter term 2012/13.		Goal of the workshop was to repurpose a possible cheap USB webcam into a microscope. Max Neupert, the organizer of this workshop, told us how to achieve this and instructed us throughout the winter term 2012/13.

	The basic concept ~~Max told us~~ is pretty simple: just disassemble the camera, mount the lens upside down and shoot some great photos with the new microscope.		The basic concept is pretty simple: just disassemble the camera, mount the lens upside down and shoot some great photos with the new microscope.

	== Disassembling the Camera ==		== Disassembling the Camera ==

	The "Hama PC-Webcam AC-150" served as a basis, which costs about € 5 when ordering via Amazon. It has a resolution of 640x480 pixels and six LEDs to lighten the scene. Beyond this, the camera supports UVC (USB Video Class), which is important for viewing live images directly on the screen.		The "Hama PC-Webcam AC-150" served as a basis, which costs about € 5 when ordering via Amazon. It has a resolution of 640x480 pixels and six LEDs to lighten the scene. Beyond this, the camera supports UVC (USB Video Class), which is important for viewing live images directly on the screen.

Line 21:		Line 19:

	== Building a Case ==		== Building a Case ==

	[[File:Microscope-case-jakob-side-view.png\|195px\|thumb\|right\|Side view]]		[[File:Microscope-case-jakob-side-view.png\|195px\|thumb\|right\|Side view]]
	[[File:Microscope-case-jakob-rendered.jpg\|195px\|thumb\|right\|Rendered image of first draft]]		[[File:Microscope-case-jakob-rendered.jpg\|195px\|thumb\|right\|Rendered image of first draft]]
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	== WebcamCapture Application ==		== WebcamCapture Application ==

	[[File:Microscope-jakob-webcam-capture-screenshot.png‎\|195px\|right\|thumb\|Screenshot of application (the display of my mobile phone served as investigation object)]]		[[File:Microscope-jakob-webcam-capture-screenshot.png‎\|195px\|right\|thumb\|Screenshot of application (the display of my mobile phone served as investigation object)]]

Max: /* WebcamCapture Application */

2012-11-25T17:11:50Z

WebcamCapture Application

← Older revision		Revision as of 17:11, 25 November 2012
Line 54:		Line 54:
	* '''N''' - Add suffix to filename (so you can identify your object later on)		* '''N''' - Add suffix to filename (so you can identify your object later on)
	* '''Escape''' - Exit application		* '''Escape''' - Exit application

			<br clear="all" />

	== First images ==		== First images ==

← Older revision		Revision as of 20:21, 3 December 2012
Line 70:		Line 70:

	<videoflash type="vimeo">54222462\|640\|480</videoflash>		<videoflash type="vimeo">54222462\|640\|480</videoflash>

			== First artistical tries ==
			[[File:Microscope-jakob-drying-condiments.jpg\|195px\|right\|thumb\|Condiments drying at a radiator)]]

			In her talk about the basics of microscopy, Adriana showed us some nice images captured with microscopes, such as the ones on [http://scienceblogs.com/retrospectacle/2007/01/30/beer-microscopy-project/ this site]. Since I liked the way the artists colored their specimen, I asked myself how to achieve a similar effect with possible cheap equipment.

			So, I considered to dye sugar, salt and meal with food coloring and then take gaudy images of it. In the photo to the right, one can see the three condiments drying at a radiator after coloring.

			And here is a gallery of some images I shot:

			<gallery perrow="4">
			File:Microscope-jakob-plain-sugar-1.png\|Usual sugar
			File:Microscope-jakob-blue-sugar-1.png\|Blue colored sugar
			File:Microscope-jakob-red-sugar-1.png\|Red colored sugar
			File:Microscope-jakob-gaudy-sugar-1.png\|Sugar in multiple colors
			File:Microscope-jakob-usual-salt-1.png\|Usual salt
			File:Microscope-jakob-blue-salt-1.png\|Blue colored salt
			File:Microscope-jakob-yellow-salt-1.png\|Yellow colored salt
			File:Microscope-jakob-gaudy-salt-1.png\|Salt in multiple colors
			File:Microscope-jakob-usual-meal-1.png\|Usual meal
			File:Microscope-jakob-green-meal-1.png\|Green colored meal
			File:Microscope-jakob-red-meal-1.png\|Red colored meal
			File:Microscope-jakob-gaudy-meal-1.png\|Meal in multiple colors
			</gallery>

			Some of the images are nice-looking, but after all I'm not satisfied with the results. One big problem I noticed when taking these photos is that the camera has an automatic white balance; so, it was the best to always keep the Petri dish in motion, which made it quite hard to shoot good photos.

			However, I will look for other exciting effects that can be achieved with our self-made microscope.