GMU:BioArt WS16/Transgenic organisms - Revision history

Miga: Miga moved page GMU:Intro to BioArt/Transgenic organisms to GMU:BioArt WS16/Transgenic organisms

2018-01-06T11:34:10Z

Miga moved page GMU:Intro to BioArt/Transgenic organisms to GMU:BioArt WS16/Transgenic organisms

← Older revision	Revision as of 11:34, 6 January 2018
(No difference)

Miga: /* GloFish */

2017-01-05T10:52:38Z

GloFish

← Older revision		Revision as of 10:52, 5 January 2017
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	===GloFish===		===GloFish===
	https://www.google.de/search?q=GloFish~~&source=lnms~~&tbm=isch~~&sa=X&ved=0ahUKEwiRvcLXmafRAhUKFywKHcB3DCEQ_AUICCgB&biw=1255&bih=799~~		https://www.google.de/search?q=GloFish&tbm=isch

	The first genetically modified animal to be sold as a pet		The first genetically modified animal to be sold as a pet

Miga: /* GFP E.coli Workshop (method 1) */

2017-01-04T12:06:39Z

GFP E.coli Workshop (method 1)

← Older revision		Revision as of 12:06, 4 January 2017
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	Needed:		Needed:
	* (~~Reptilian~~) incubator		* (Reptile) incubator
	* Spectrometer to calculate cell growth mass		* Spectrometer to calculate cell growth mass
	TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water.		TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water.

Miga: /* GFP E.coli Workshop (method 1) */

2017-01-04T12:06:00Z

GFP E.coli Workshop (method 1)

← Older revision		Revision as of 12:06, 4 January 2017
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	Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night		Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night

			Day 3

			https://www.google.de/search?q=gfp+e+coli&tbm=isch

	== References ==		== References ==
	* wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis		* wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis
	* wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting		* wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting

Miga: /* GFP E.coli Workshop (method 1) */

2017-01-04T08:16:13Z

GFP E.coli Workshop (method 1)

← Older revision		Revision as of 08:16, 4 January 2017
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	* (Reptilian) incubator		* (Reptilian) incubator
	* Spectrometer to calculate cell growth mass		* Spectrometer to calculate cell growth mass
	TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ~~eis~~ and then heat shock with 42 degrees warmed up water.		TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water.

	Day 2		Day 2

Miga: /* GFP E.coli Workshop (method 1) */

2017-01-04T08:15:08Z

GFP E.coli Workshop (method 1)

← Older revision		Revision as of 08:15, 4 January 2017
Line 75:		Line 75:
	* (Reptilian) incubator		* (Reptilian) incubator
	* Spectrometer to calculate cell growth mass		* Spectrometer to calculate cell growth mass
	TSS medium reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the eis and then heat shock with 42 degrees warmed up water.		TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the eis and then heat shock with 42 degrees warmed up water.

	Day 2		Day 2

	Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night		Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night

	== References ==		== References ==
	* wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis		* wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis
	* wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting		* wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting

Miga: /* Methods for genetically modified plants */

2017-01-04T08:12:49Z

Methods for genetically modified plants

← Older revision		Revision as of 08:12, 4 January 2017
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	"Among the most important tools in the genetic engineer's tool kit are enzymes that perform specific functions on DNA. .. enzymes known as ligases join the ends of two DNA fragments. These and other enzymes enable the manipulation and amplification of DNA, essential components in joining the DNA of two unrelated organisms."(Fenwick 2004)		"Among the most important tools in the genetic engineer's tool kit are enzymes that perform specific functions on DNA. .. enzymes known as ligases join the ends of two DNA fragments. These and other enzymes enable the manipulation and amplification of DNA, essential components in joining the DNA of two unrelated organisms."(Fenwick 2004)

	===Methods for genetically modified ~~plants~~===		===Methods for genetically modified organisms===
	1. man mischt vereinzelte Zellen mit DNA Molekülen / eg using heat stress		1. man mischt vereinzelte Zellen mit DNA Molekülen / eg using heat stress

Miga: /* Eduardo Kac */

2017-01-04T08:01:43Z

Eduardo Kac

← Older revision		Revision as of 08:01, 4 January 2017
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	===Eduardo Kac===		===Eduardo Kac===
	GFP Bunny. http://www.ekac.org/gfpbunny.html		GFP Bunny, 2000 http://www.ekac.org/gfpbunny.html#gfpbunnyanchor

	Edunia. ~~Source:~~ ekac.org		"My transgenic artwork "GFP Bunny" comprises the creation of a green fluorescent rabbit, the public dialogue generated by the project, and the social integration of the rabbit."

			Edunia, http://www.ekac.org/nat.hist.enig.html

	Natural history of the Enigma. Edunia. "The new flower is a Petunia strain that I invented and produced through molecular biology. It is not found in nature. The Edunia has red veins on light pink petals and a gene of mine is expressed on every cell of its red veins, i.e., my gene produces a protein in the veins only [2]. The gene was isolated and sequenced from my blood. The petal pink background, against which the red veins are seen, is evocative of my own pinkish white skin tone. The result of this molecular manipulation is a bloom that creates the living image of human blood rushing through the veins of a flower." http://www.ekac.org/nat.hist.enig.html		Natural history of the Enigma. Edunia. "The new flower is a Petunia strain that I invented and produced through molecular biology. It is not found in nature. The Edunia has red veins on light pink petals and a gene of mine is expressed on every cell of its red veins, i.e., my gene produces a protein in the veins only [2]. The gene was isolated and sequenced from my blood. The petal pink background, against which the red veins are seen, is evocative of my own pinkish white skin tone. The result of this molecular manipulation is a bloom that creates the living image of human blood rushing through the veins of a flower." http://www.ekac.org/nat.hist.enig.html

Miga: /* Related projects */

2017-01-04T07:59:14Z

Related projects

← Older revision		Revision as of 07:59, 4 January 2017
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	The first genetically modified animal to be sold as a pet		The first genetically modified animal to be sold as a pet

	~~===Revital Cohen and Tuur Van Balen===~~
	~~Sterile, http://www.cohenvanbalen.com/work/sterile~~

	In Sterile, albino goldfish were engineered to hatch without reproductive organs. Following a long collaboration, an edition of 45 goldfish was produced for the artists by Professor Yamaha in his laboratory in Hokkaido, Japan. The fish were not conceived as animals but made as objects, unable to partake in the biological cycle. http://www.cohenvanbalen.com/work/sterile

	===Eduardo Kac===		===Eduardo Kac===
Line 20:		Line 15:
	Natural history of the Enigma. Edunia. "The new flower is a Petunia strain that I invented and produced through molecular biology. It is not found in nature. The Edunia has red veins on light pink petals and a gene of mine is expressed on every cell of its red veins, i.e., my gene produces a protein in the veins only [2]. The gene was isolated and sequenced from my blood. The petal pink background, against which the red veins are seen, is evocative of my own pinkish white skin tone. The result of this molecular manipulation is a bloom that creates the living image of human blood rushing through the veins of a flower." http://www.ekac.org/nat.hist.enig.html		Natural history of the Enigma. Edunia. "The new flower is a Petunia strain that I invented and produced through molecular biology. It is not found in nature. The Edunia has red veins on light pink petals and a gene of mine is expressed on every cell of its red veins, i.e., my gene produces a protein in the veins only [2]. The gene was isolated and sequenced from my blood. The petal pink background, against which the red veins are seen, is evocative of my own pinkish white skin tone. The result of this molecular manipulation is a bloom that creates the living image of human blood rushing through the veins of a flower." http://www.ekac.org/nat.hist.enig.html

			===Revital Cohen and Tuur Van Balen===
			Sterile, http://www.cohenvanbalen.com/work/sterile

	~~===Super Cell===~~		In Sterile, albino goldfish were engineered to hatch without reproductive organs. Following a long collaboration, an edition of 45 goldfish was produced for the artists by Professor Yamaha in his laboratory in Hokkaido, Japan. The fish were not conceived as animals but made as objects, unable to partake in the biological cycle. http://www.cohenvanbalen.com/work/sterile
	~~The project Super Cell is~~ a ~~fictional supermarket offering speculative products~~, ~~which are all based on Synthetic Biology~~. The ~~projects aims to improve public awareness about Synthetic Biology and at the same time~~, ~~foster a debate on how we want~~ to ~~see it manifested~~ in ~~our everyday lives~~. ~~Implications of Biotechnologies are usually discussed on a philosophical, ethical, legal or possibly religious level. https~~://~~vimeo~~.com/~~39653216 http://super-cell.org~~/

	===Joe Davis===		===Joe Davis===

Miga at 00:23, 4 January 2017

2017-01-04T00:23:13Z

← Older revision		Revision as of 00:23, 4 January 2017
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	~~Transgenic organisms~~

	==Related projects==		==Related projects==
	===mythological hybrids===		===mythological hybrids===