Medien Wiki - User contributions [en]

GMU:DIY BioLab

2020-11-16T15:57:26Z

Sarah Spitzer: /* Booking */

Marienstraße 7b, Room 202, 99423 Weimar

Contact: Mindaugas Gapševičius, mindaugas.gapsevicius [at] uni-weimar.de, Julian Chollet, julian [at] mikrobiomik.org 

= Booking =

To reserve a workspace, please write your name and the Nr. of the workspace next to the timeslot. 2 Students can work in the lab at the same time. For example: Julian (#1); Miga (#2). Depending on the availability, you can also book the Biolab for 2 or more hours. If you need an introduction to the hardware - please specify it in brackets. When one of the supervisors is present, the lab can be used by more than 2 people. Please read the Hygiene concept (below) for more information.

Workspace #1 includes the Lab-Workbenches and the Binocular-Microscope ("the small one") 
Workspace #2 includes the the two small tables, a computer and the Bresser-Microscope ("the big one")

'''Timeslots''' 
18.11.2020 (supervised by Miga)

10:00 - 11:00 – (put your name here) / (put your name here) 
11:00 - 12:00 – (put your name here) / (put your name here) 
12:00 - 13:00 – (put your name here) / (put your name here) 
15:00 - 16:00 – (put your name here) / (put your name here) 
16:00 - 17:00 – (put your name here) / (put your name here) 
17:00 - 18:00 – (put your name here) / (put your name here) 
 

19.11.2020 (supervised by Miga)

10:00 - 11:00 – (put your name here) / (put your name here) 
11:00 - 12:00 – (put your name here) / (put your name here) 
12:00 - 13:00 – (put your name here) / (put your name here) 
15:00 - 16:00 – (put your name here) / (put your name here) 
16:00 - 17:00 – (put your name here) / (put your name here) 
17:00 - 18:00 – (put your name here) / (put your name here) 
 

23.11.2020 (supervised by Julian)

10:00 - 11:00 – Sarah Spitzer (#1) (introduction to microscopes) / (put your name here) 
11:00 - 12:00 – (put your name here) / (put your name here) 
12:00 - 13:00 – (put your name here) / (put your name here) 
15:00 - 16:00 – (put your name here) / (put your name here) 
16:00 - 17:00 – (put your name here) / (put your name here) 
17:00 - 18:00 – (put your name here) / (put your name here) 
 

24.11.2020 (supervised by Julian)

10:00 - 11:00 – (put your name here) / (put your name here) 
11:00 - 12:00 – (put your name here) / (put your name here) 
12:00 - 13:00 – (put your name here) / (put your name here) 
15:00 - 16:00 – (put your name here) / (put your name here) 

 
 
 
 

== Hygienekonzept (deutsch) ==
als pdf 
[[:File:Hygienekonzept-DIY-Biolab-(03.09.20)_1page.pdf]]

Kurzfassung
* Digitale Anmeldung durch Studierende erfolgt per Mail und im Online-Studiokalender im Moodle.

* der/die Mitarbeiter/in informiert Studierende über Hygieneregeln (Belehrung wird unterschrieben)

* der/die Mitarbeiter/in informiert das Sekretariat MKG über die Freischaltung
(Name, Zeitraum der Freischaltung, erfolgte Belehrung).

* Das Sekretariat MKG schaltet Studierende für den vereinbarten Zeitraum über das Schließsystem am Haupteingang frei.

* Erfassung der Anwesenheit der Studierende über die Belegungsliste Handelsübliche Reinigung steht in den Räumlichkeiten bereit.

* Vor dem Verlassen muss der Raum ausreichend gelüftet werden.

* Die Laufwege für das Verlassen des DIY Biolabs sind am Boden markiert.

* Arbeitsplätze und Mindestabstände werden gekennzeichnet mit Arbeitsplatz 1 und 2.

* Die Einhaltung der Hygieneregelungen werden regelmäßig geprüft.

* bei Nichteinhaltung erlischt die Erlaubnis zur Nutzung des Raumes für den Studierende und die Freischaltung wird deaktiviert.

 

== Short introduction to our DIY Bio-Lab ==

Welcome to our DIY Bio-Lab at Bauhaus University Weimar

Before you start using our microscopes, the laminar hood or chemicals, please take a couple of hours to prepare beforehand:

* read the manuals for the microscopes and understand how to use them
* watch two videos and learn how to use microliter pipettes
* get some basic vocabulary about lab- equipment
* read some recipes for the medium your organism needs
* and read the basic papers about your organism, before you start growing it.

===Microscope===
* [http://www.ehlert-partner.de/Mikroskopreinigung.pdf das saubere Mikroskop]
*'''The Bresser Microscope (''the big one'')''' [https://www.bresser.de/out/media/d32560c4539eb0c24bee07dbb7f2c40e.pdf Manual ]

<gallery>
File:SomeniceBacteria.png|these kind of pictures are possible with the Bresser microscope. Full rez SomeniceBacteria.JPG
File:Moldondictyostelium.png|another one of common mold spore heads. Full rez Moldondictyostelium.JPG
</gallery>

* [http://www.masterflex.com/assets/Manual_pdfs/48925-30%20-35%20-40.pdf The Masterflex microscope ("the small one")]
* [http://www.kanidis.gr/common/files/MOLECULAR/EQUIPMENT/Cat_V100_i.pdf The laminar hood]

===microliter pipettes===
*[https://www.youtube.com/watch?v=NgosWmRjjAo Micropipetting]
*[https://www.youtube.com/watch?v=nv5Bi2Wy1KU School Pipetting 101 Common Errors]

===vocabulary===
*[http://en.wikipedia.org/wiki/Category:Laboratory_equipment Laboratory equipment]
*[http://www.sciencegeek.net/Chemistry/taters/labequipment.htm Lab Equipment Quiz]
*[http://en.wikipedia.org/wiki/PH pH]

===Medium===
What kind of medium you want to prepare and what your organism needs you have to find out yourself.

See available [[/chemicals]] in the lab.

== Current Organisms ==
*[[GMU:Habitats_SS18/Physarum_Polycephalum|Physarum Polycephalum]]
*[http://www.schulbiologiezentrum.info/15.20%20Euglena%20Neufassung%20031203.pdf Euglena]
*[[GMU:Experimental_Microbiology/Amoeba_proteus|Amoeba proteus]]
*[[GMU:Experimental_Microbiology/Armillaria_gallica|Armillaria gallica]]
*[[GMU:Experimental_Microbiology/Hydra Vulgaris|Hydra_Vulgaris]]
*[[GMU:Experimental_Microbiology/Haematococcus_pluvialis|Haematococcus pluvialis]]

If you want to work with one of these organisms, please present a short concept to GMU Staff during opening hours.
If you want to work with another organism please contact us.

[http://zooundco-brehm.de/unsere-maerkte/weimar/? Animal Store in Weimar ]

This store has '''living''' Artemia, Daphnia, Nematocera (Chironomus & Chaoborus) and Enchytraeen ( Enchytraeus albidus). They sell them in small bags and it costs less than a coffee. Each Tuesday they will have a new delivery of these fresh organisms.
It is possible for them to get other 'fish food' as well.

=== Protocols ===
* [[/Euglena|Euglena]]
* [[/Amoeba|Amoeba]]
* [[/Physarum Polycephalum|Physarum Polycephalum]]
* [[/Dictyostelium|Disctyostelium]]

== Further Information ==
=== Research projects ===
*[[GMU:PhyChip|PhyChip]]
*[[GMU:Biogames|Biogames]]

=== Current Courses and Activities around Biolab ===
*[[GMU:Experimental_Microbiology|Experimental Microbiology]]
*[[GMU:Bioelectronics,_aesthetics_and_other_interesting_things|Bioelectronics, aesthetics and other interesting things]]
*[[GMU:Genes%26Clones|Genes&Clones]]

===Latest Courses===
*[[GMU:Experimental_Microbiology|Experimental Microbiology]]
*[[GMU:BioArt|BioArt]]
*[[GMU:Bioart_Introduction|Bioart_Introduction]]
*[[GMU:BioGames|BioGames]]

===Latest workshops===
*[[GMU:MediaArt_and_Biotechnology|MediaArt and Biotechnology]]
*[[GMU:Workshop on BioGames|Workshop on BioGames]]
*[[GMU:BioMedia_Science_2_Arts-_Molecular_Biology_entering_the_public_space|BioMedia Science 2 Arts- Molecular Biology entering the public space]]

=== Links ===
*[http://www.diysect.com/ Diysect Webseries about BioArt]

== Photos ==
<gallery>
File:IMG_4335.png|Photo: miga
File:DSC_0197.png|Photo: Smin Kim
File:DSC_0199.png|Photo: Smin Kim
File:DSC_0203.png|Photo: Smin Kim
File:DSC_0200-1.png|Photo:miga
File:DSC_0202-1.png|Photo:miga
File:DSC_0206.png|Photo:miga
File:IMG_1368.png|Photo:miga
File:IMG_1371.png|Photo:miga
</gallery>

GMU:Introduction to Microscopy

2020-11-16T15:48:56Z

Sarah Spitzer: /* Students */

[[:Category:Werkmodul|Werkmodul]] [[:Category:Fachmodul|Fachmodul]]

[[File:microscopy.jpg|400px|thumb|left]]

''Lecturer:'' [[Julian Chollet]] 
''Credits:'' 6 [[ECTS]], 4 [[SWS]] 
''Date:'' Monday (15:15 – 16:45) + individual trainings 
''Venue:'' Monday-meeting online / trainings in [[Marienstraße 7b]], [[GMU:DIY BioLab|DIY BioLab]] (Room 202) 

 

==Students==
*[[GMU:Introduction_to_Microscopy/Sascha_Mirtschin|Sascha Mirtschin]]
*[[GMU:Introduction_to_Microscopy/Mon_Sisu_Satrawaha|Mon Sisu Satrawaha]]
*[[GMU:Introduction_to_Microscopy/Anna_Strietzel|Anna Strietzel]]
*[[GMU:Introduction_to_Microscopy/Funda_Zeynep_Ayguler|Funda Zeynep Ayguler]]
*[[GMU:Flagelates,_Nematodes,_and_I/Brogen_Berwick|Brogen Berwick]]
*[[GMU:Introduction_to_Microscopy/Eirini_Kokkinidou|Eirini Kokkinidou]]
*[[GMU:Introduction_to_Microscopy/Denise_Nicoau|Denise Nicolau]]
*[[GMU:Introduction_to_Microscopy/Sarah_Spitzer|Sarah Spitzer]]

==Videos==
Great introduction to light microscopy (not only) for kids: 
https://www.youtube.com/watch?v=tVcEEw6qbBQ

==Syllabus==
Our air, soil and water as well as all plants and animals contain complex ecosystems that
are colonized by various creatures - most of which we can only see and interact with by
virtue of microscopy or cultivation techniques.

This course aims to introduce you to the methodologies, project planning and experimental strategies in the natural sciences – mainly through the use of microscopy techniques. In line with the concept of a hybrid semester, the theoretical input will be communicated through online lectures while the practical skills will be acquired in individualized block-modules. While working in the DIY BioLab (Chair of Media Environments) you will learn how to use different kinds of microscopes and start your own little research project. Beyond the microscopy itself, educational objectives include literature research, experiment design, result documentation and scientific writing.

The course will be structured in a flexible way, tailored to the needs of the participants. Attendance during the online-seminars and hands-on module, as well as the delivery of detailed project documentation (paper, artwork, etc.) until the end of the semester is required. The course can be combined with the module “Flagelates, Nematodes, and I” by Mindaugas Gapševičius.

[[Category:WS18]]
[[Category:Werkmodul]]
[[Category:Fachmodul]]
[[Category:Julian Chollet]]

==Basic concepts==
===Life===
"There are over a 100 definitions for 'life' and all are wrong" 
http://www.bbc.com/earth/story/20170101-there-are-over-100-definitions-for-life-and-all-are-wrong

https://en.wikipedia.org/wiki/Life#Definitions 
Since there is no unequivocal definition of life, most current definitions in biology are descriptive. Life is considered a characteristic of something that preserves, furthers or reinforces its existence in the given environment. This characteristic exhibits all or most of the following traits:
Homeostasis
Organization
Metabolism
Growth
Adaptation
Response to stimuli
Reproduction

===Organism===
https://en.wikipedia.org/wiki/Organism 
In biology, an organism (from Greek: ὀργανισμός, organismos) is any individual entity that exhibits the properties of life. It is a synonym for "life form".
Organisms are classified by taxonomy into specified groups such as the multicellular animals, plants, and fungi; or unicellular microorganisms such as a protists, bacteria, and archaea. All types of organisms are capable of reproduction, growth and development, maintenance, and some degree of response to stimuli.

====Prokaryote====
Prokaryotes are represented by two separate domains—bacteria and archaea.
A unicellular organism that lacks a membrane-bound nucleus, mitochondria, or any other membrane-bound organelle.

====Eukaryote====
Eukaryotic organisms are characterized by the presence of a membrane-bound cell nucleus and contain additional membrane-bound compartments called organelles (such as mitochondria in animals and plants and plastids in plants and algae, all generally considered to be derived from endosymbiotic bacteria). Fungi, animals and plants are examples of kingdoms of organisms within the eukaryotes.

===Microorganism===
Living beings that are too small to be seen with the (human) eye.

https://en.wikipedia.org/wiki/Microorganism 
https://en.wikipedia.org/wiki/Human_interactions_with_microbes

They live in almost every habitat from the poles to the equator, deserts, geysers, rocks and the deep sea. Some are adapted to extremes such as very hot or very cold conditions, others to high pressure or [...] high radiation environments. Microorganisms also make up the microbiota found in and on all multicellular organisms.

Microbes are important in human culture and health in many ways, serving to ferment foods, treat sewage, produce fuel, enzymes and other bioactive compounds. They are essential tools in biology as model organisms and have been put to use in biological warfare and bioterrorism. They are a vital component of fertile soils. In the human body microorganisms make up the human microbiota including the essential gut flora. They are the pathogens responsible for many infectious diseases and as such are the target of hygiene measures.

The possible existence of unseen microbial life was suspected from ancient times, such as in Jain scriptures from 6th century BC India and the 1st century BC book On Agriculture by Marcus Terentius Varro. Microbiology, the scientific study of microorganisms, began with their observation under the microscope in the 1670s by Antonie van Leeuwenhoek.

==Suggested resources regarding the proposed projects/topics==

===Cytoplasmic streaming===

==PDFs==
[[:File:Guide_to_scientific_writing.pdf]] 

==Links==
* [[GMU:DIY BioLab|DIY BioLab]]
* [https://mikroBIOMIK.org/en mikroBIOMIK]

[[Category:WS20]][[Category:Julian Chollet]]