https://www.uni-weimar.de/kunst-und-gestaltung/wiki/api.php?action=feedcontributions&feedformat=atom&user=Lea.maravic 2026-04-09T09:03:09Z User contributions MediaWiki 1.39.6 https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136158 2023-07-21T19:31:25Z

<p>Lea.maravic: new photo</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]]<br /> [[File:20230521 115319.jpg|thumb|341x341px|Setting up technology[[File:Nanopore active cells.jpg|thumb|345x345px|Nanopore active cells]]]]<br /> <br /> <br /> <br /> '''WORKSHOP III.'''<br /> <br /> 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample[[File:Comparing the results with online libraries.jpg|thumb|345x345px|Comparing the results with online libraries]]]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing.<br /> <br /> Once in the laboratory we extracted small amounts of the fluid from multiple cyanobacteria and algae cultures. We then prepared the sample by adding various buffers according to the prescribed protocol.<br /> <br /> The sample contained many organisms and once the sequencing was done we could compare the results to the information in online libraries and identify the organisms present in our sample.</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Comparing_the_results_with_online_libraries.jpg&diff=136156 2023-07-21T19:30:59Z

<p>Lea.maravic: </p> <hr /> <div>Comparing the results with online libraries</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136155 2023-07-21T19:29:11Z

<p>Lea.maravic: new changes</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]]<br /> [[File:20230521 115319.jpg|thumb|341x341px|Setting up technology[[File:Nanopore active cells.jpg|thumb|345x345px|Nanopore active cells]]]]<br /> <br /> <br /> <br /> '''WORKSHOP III.'''<br /> <br /> 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing.<br /> <br /> Once in the laboratory we extracted small amounts of the fluid from multiple cyanobacteria and algae cultures. We then prepared the sample by adding various buffers according to the prescribed protocol.<br /> <br /> The sample contained many organisms and once the sequencing was done we could compare the results to the information in online libraries and identify the organisms present in our sample.</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Nanopore_active_cells.jpg&diff=136152 2023-07-21T19:28:13Z

<p>Lea.maravic: </p> <hr /> <div>Nanopore active cells</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136150 2023-07-21T19:17:09Z

<p>Lea.maravic: </p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]]<br /> [[File:20230521 115319.jpg|thumb|341x341px|Setting up technology]]<br /> <br /> <br /> <br /> '''WORKSHOP III.'''<br /> <br /> 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing.</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:20230521_115319.jpg&diff=136149 2023-07-21T19:15:43Z

<p>Lea.maravic: </p> <hr /> <div>Setting up technology</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136148 2023-07-21T19:14:59Z

<p>Lea.maravic: workshop 3</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]]<br /> <br /> <br /> <br /> '''WORKSHOP III.'''<br /> <br /> 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim<br /> <br /> Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing.<br /> [[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample]]</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:MinION_sequencer_with_flow_cell.jpg&diff=136147 2023-07-21T19:11:37Z

<p>Lea.maravic: </p> <hr /> <div>MinION sequencer with flow cell that is being loaded with the sample</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:MinION_sequencer_with_flow_cell_that_is_being_loaded_with_the_sample.jpg&diff=136138 2023-07-21T19:04:30Z

<p>Lea.maravic: </p> <hr /> <div>MinION sequencer with flow cell that is being loaded with the sample</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136130 2023-07-21T18:39:18Z

<p>Lea.maravic: NEW CHANGES</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]]<br /> <br /> <br /> '''WORKSHOP III.'''<br /> <br /> 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136129 2023-07-21T18:34:28Z

<p>Lea.maravic: new photo</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]]</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Results.jpg&diff=136128 2023-07-21T18:33:39Z

<p>Lea.maravic: </p> <hr /> <div>Shared photo of the results</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136127 2023-07-21T18:29:47Z

<p>Lea.maravic: new change</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.<br /> [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2]]</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:20230428_134817.jpg&diff=136126 2023-07-21T18:28:30Z

<p>Lea.maravic: </p> <hr /> <div>termocycler</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136125 2023-07-21T18:27:35Z

<p>Lea.maravic: added image</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet<br /> <br /> Julian is a molecular biologist who showed us how to use a diagnostic tool called PCR (Polymerase Chain Reaction) for DNA analysis. All living beings contain a complex molecule called DNA polymerase. It copies DNA so all organisms that want to replicate have it. In order to replicate and or analyse a DNA in a laboratory we need 3 substances: DNA or an organism we want to analyse, DNA polymerase and primers. Primers are small pieces of DNA made up in a laboratory and they can have any sequence of nucleotides. <br /> <br /> For the experiment all the participants chose a substance to analyse and then we prepared the specimens. Mostly we worked with fruit. Some of us chose saliva as our specimen. Most specimens were prepared by first chopping, blending, mushing and centrifuging them. Then we put them through 3 stages:<br /> <br /> 1) DENATURATION - heating to 95°C to break up the connections between DNA helyx<br /> <br /> 2) ANNEALING - at the temperature of 55°C primers will attach to the basis of DNA <br /> <br /> 3) EXTENSION - the temperature of 72°C is the point when DNA polymerase replicates DNA by extending the primer. It assembles 1000 pieces of DNA at this temperature<br /> [[File:20230428 134426.jpg|thumb|1082x1082px|Preparing samples]]<br /> After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test.</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:20230428_134426.jpg&diff=136124 2023-07-21T18:25:39Z

<p>Lea.maravic: </p> <hr /> <div>photo test</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:20230428_121601.jpg&diff=136111 2023-07-21T17:49:20Z

<p>Lea.maravic: </p> <hr /> <div>a photo</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136108 2023-07-21T17:28:36Z

<p>Lea.maravic: bold text</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;'''WORKSHOP II.'''<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Preparing_samples_for_DNA_analysis.jpg&diff=136106 2023-07-21T17:25:31Z

<p>Lea.maravic: </p> <hr /> <div>We each chose different organic material and prepared the sample for the analysis</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136105 2023-07-21T17:22:35Z

<p>Lea.maravic: Added a title to the second workshop</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;WORKSHOP II.<br /> <br /> 28.04.2023, 29.04.2023 - [[GMU:BioArt Forum/DNA analysis|/DNA analysis]] with molecular biologist Julian Chollet</div>

Lea.maravic https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:BioArt_Forum/Lea_Maravic&diff=136102 2023-07-21T17:12:53Z

<p>Lea.maravic: Added the title to the gallery</p> <hr /> <div>'''WORKSHOP I'''<br /> <br /> 12.04.2023, 13.04.2023 - [[GMU:BioArt Forum/Growing, Shaping and Living (with) Microalgae|/Growing, Shaping and Living (with) Microalgae]] with biologist Johann Bauerfeind&lt;gallery&gt;<br /> File:20230412_105730.jpg<br /> File:20230412_111428.jpg<br /> File:20230412_143503.jpg<br /> File:20230412_150047.jpg<br /> File:20230412_150053.jpg<br /> File:20230412_150130.jpg<br /> File:20230412_150142.jpg<br /> File:20230412_150152.jpg<br /> File:20230412_150241.jpg<br /> File:20230412_150512.jpg<br /> File:20230412_150517.jpg<br /> File:20230412_150611.jpg<br /> File:20230412_150634.jpg<br /> File:20230412_150645.jpg<br /> File:20230412_150903.jpg<br /> File:20230412_151247.jpg<br /> File:20230412_151352.jpg<br /> File:20230412_153223.jpg<br /> File:20230412_153729.jpg<br /> File:20230412_154114.jpg<br /> File:20230412_154315.jpg<br /> File:20230412_154332.jpg<br /> File:20230412_154347.jpg<br /> File:20230412_154742.jpg<br /> File:20230412_154815.jpg<br /> File:20230413_121204.jpg<br /> File:20230413_121311.jpg<br /> File:20230417_110858.jpg<br /> File:20230418_171027.jpg<br /> &lt;/gallery&gt;</div>

Lea.maravic