https://www.uni-weimar.de/kunst-und-gestaltung/wiki/api.php?action=feedcontributions&feedformat=atom&user=Jan+Gl%C3%B6ckner 2026-04-23T20:13:39Z User contributions MediaWiki 1.39.6 https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:Habitats_SS18/Jan_Georg_Gl%C3%B6ckner&diff=97474 2018-06-06T11:25:45Z

<p>Jan Glöckner: </p> <hr /> <div>'''Life-Support-Systems'''<br /> <br /> <br /> '''Abstract'''<br /> <br /> <br /> I define systems that serve the purpose of sustaining live of the participating organisms as life-support-systems. This definition distinguishes life-support-systems from the term habitat, which is the natural environment of an organism, in which it can find food, shelter and reproduce. Life-support-systems are artifical and meant to stabilize the parameters of life of the participating organisms. This can include parameters from a natural enviromnent, maybe even mimicking it, but it is not the directive of a life-support-system. <br /> <br /> When an organisms wants to leave its natural habitat and colonize or travel to another habitat in which it cannot sustain its life-parameters, it needs a life-support-system. In fiction there are interesting examples of this bare necessity: from Dracula, who has to sleep in soil of his homeland, that he ships in coffins to London so he can sustain his life there, to the fremen-suits of the natives of Arakis, the desert planet in Frank Herbert‘s Dune, who use sophisticated wearables to survive in conditions of extreme heat and draught.<br /> <br /> My intention is to design a life-support-system that will sustain the life of a fungus for as long as possible. <br /> <br /> Based on the theory that a fungus will grow indefinetly, as long as it is provided with enough food and has no physical boundaries¹, I grow Pleurotus Ostreatus and Pleurotus Djamor in liquid culture. This theory opposes the opinion that fungi are subject to a process called senescence or biological ageing that leads to loss in vigor and the eventual death of a individuum².<br /> <br /> <br /> &lt;div style=&quot;color:#72bf44;&quot;&gt;'''Directive 1:'''&lt;/div&gt;<br /> <br /> <br /> Let the fungi grow in a shape that makes it easier for hominina to relate to it. The shape should be appealing and spark empathy.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 2:'''&lt;/div&gt;<br /> <br /> <br /> Investigate and search for partners to the fungi, that can supply the needed resources to sustain life.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 3: '''&lt;/div&gt;<br /> <br /> <br /> Design a system that sustains the live of the fungi and their partner for as long as possible.<br /> <br /> <br /> &lt;div style=&quot;color:#ed1c24;&quot;&gt;Directive 4:&lt;/div&gt;<br /> <br /> Prove that there is no such thing as senescence for fungi and that the effect described as such can be prevented in a laboratory set-up.<br /> <br /> <br /> '''Methodology'''<br /> <br /> <br /> Two fungi in the same genus Pleurotus ostreatus and djamor are cultivated in liquid culture. The formation of clusters is controlled by media formulation and by pressure of the aeriation-system.<br /> <br /> [[File:0516_1.jpg|400px]]<br /> [[File:sphere.JPG|400px]]<br /> <br /> <br /> <br /> '''¹''' see page 206 ''Mycelium is a hologram''&lt;nowiki&gt;; &lt;/nowiki&gt;'''Radical Mycology'''&lt;nowiki&gt;; Peter McCoy &lt;/nowiki&gt;Chthaeus Press ISBN 978-0-9863996-0-2<br /> <br /> '''² '''see page Growing Gourmet and Medicinal Mushrooms Paul Stamets</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:0516_1.jpg&diff=97473 2018-06-06T11:25:14Z

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Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:Habitats_SS18/Jan_Georg_Gl%C3%B6ckner&diff=97472 2018-06-06T11:23:54Z

<p>Jan Glöckner: </p> <hr /> <div>'''Life-Support-Systems'''<br /> <br /> <br /> '''Abstract'''<br /> <br /> <br /> I define systems that serve the purpose of sustaining live of the participating organisms as life-support-systems. This definition distinguishes life-support-systems from the term habitat, which is the natural environment of an organism, in which it can find food, shelter and reproduce. Life-support-systems are artifical and meant to stabilize the parameters of life of the participating organisms. This can include parameters from a natural enviromnent, maybe even mimicking it, but it is not the directive of a life-support-system. <br /> <br /> When an organisms wants to leave its natural habitat and colonize or travel to another habitat in which it cannot sustain its life-parameters, it needs a life-support-system. In fiction there are interesting examples of this bare necessity: from Dracula, who has to sleep in soil of his homeland, that he ships in coffins to London so he can sustain his life there, to the fremen-suits of the natives of Arakis, the desert planet in Frank Herbert‘s Dune, who use sophisticated wearables to survive in conditions of extreme heat and draught.<br /> <br /> My intention is to design a life-support-system that will sustain the life of a fungus for as long as possible. <br /> <br /> Based on the theory that a fungus will grow indefinetly, as long as it is provided with enough food and has no physical boundaries¹, I grow Pleurotus Ostreatus and Pleurotus Djamor in liquid culture. This theory opposes the opinion that fungi are subject to a process called senescence or biological ageing that leads to loss in vigor and the eventual death of a individuum².<br /> <br /> <br /> &lt;div style=&quot;color:#72bf44;&quot;&gt;'''Directive 1:'''&lt;/div&gt;<br /> <br /> <br /> Let the fungi grow in a shape that makes it easier for hominina to relate to it. The shape should be appealing and spark empathy.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 2:'''&lt;/div&gt;<br /> <br /> <br /> Investigate and search for partners to the fungi, that can supply the needed resources to sustain life.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 3: '''&lt;/div&gt;<br /> <br /> <br /> Design a system that sustains the live of the fungi and their partner for as long as possible.<br /> <br /> <br /> &lt;div style=&quot;color:#ed1c24;&quot;&gt;Directive 4:&lt;/div&gt;<br /> <br /> Prove that there is no such thing as senescence for fungi and that the effect described as such can be prevented in a laboratory set-up.<br /> <br /> <br /> '''Methodology'''<br /> <br /> <br /> Two fungi in the same genus Pleurotus ostreatus and djamor are cultivated in liquid culture. The formation of clusters is controlled by media formulation and by pressure of the aeriation-system.<br /> <br /> [[File:sphere.JPG|400px]]<br /> <br /> <br /> <br /> '''¹''' see page 206 ''Mycelium is a hologram''&lt;nowiki&gt;; &lt;/nowiki&gt;'''Radical Mycology'''&lt;nowiki&gt;; Peter McCoy &lt;/nowiki&gt;Chthaeus Press ISBN 978-0-9863996-0-2<br /> <br /> '''² '''see page Growing Gourmet and Medicinal Mushrooms Paul Stamets</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Sphere.JPG&diff=97471 2018-06-06T11:17:43Z

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Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:Habitats_SS18/Jan_Georg_Gl%C3%B6ckner&diff=97470 2018-06-06T11:15:29Z

<p>Jan Glöckner: </p> <hr /> <div>'''Life-Support-Systems'''<br /> <br /> <br /> '''Abstract'''<br /> <br /> <br /> I define systems that serve the purpose of sustaining live of the participating organisms as life-support-systems. This definition distinguishes life-support-systems from the term habitat, which is the natural environment of an organism, in which it can find food, shelter and reproduce. Life-support-systems are artifical and meant to stabilize the parameters of life of the participating organisms. This can include parameters from a natural enviromnent, maybe even mimicking it, but it is not the directive of a life-support-system. <br /> <br /> When an organisms wants to leave its natural habitat and colonize or travel to another habitat in which it cannot sustain its life-parameters, it needs a life-support-system. In fiction there are interesting examples of this bare necessity: from Dracula, who has to sleep in soil of his homeland, that he ships in coffins to London so he can sustain his life there, to the fremen-suits of the natives of Arakis, the desert planet in Frank Herbert‘s Dune, who use sophisticated wearables to survive in conditions of extreme heat and draught.<br /> <br /> My intention is to design a life-support-system that will sustain the life of a fungus for as long as possible. <br /> <br /> Based on the theory that a fungus will grow indefinetly, as long as it is provided with enough food and has no physical boundaries¹, I grow Pleurotus Ostreatus and Pleurotus Djamor in liquid culture. This theory opposes the opinion that fungi are subject to a process called senescence or biological ageing that leads to loss in vigor and the eventual death of a individuum².<br /> <br /> <br /> &lt;div style=&quot;color:#72bf44;&quot;&gt;'''Directive 1:'''&lt;/div&gt;<br /> <br /> <br /> Let the fungi grow in a shape that makes it easier for hominina to relate to it. The shape should be appealing and spark empathy.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 2:'''&lt;/div&gt;<br /> <br /> <br /> Investigate and search for partners to the fungi, that can supply the needed resources to sustain life.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 3: '''&lt;/div&gt;<br /> <br /> <br /> Design a system that sustains the live of the fungi and their partner for as long as possible.<br /> <br /> <br /> &lt;div style=&quot;color:#ed1c24;&quot;&gt;Directive 4:&lt;/div&gt;<br /> <br /> Prove that there is no such thing as senescence for fungi and that it can be prevented in a laboratory set-up.<br /> <br /> <br /> '''Methodology'''<br /> <br /> <br /> Two fungi in the same genus Pleurotus ostreatus and djamor are cultivated in liquid culture. The formation of clusters is controlled by media formulation and by pressure of the aeriation-system.<br /> <br /> <br /> <br /> <br /> '''¹''' see page 206 ''Mycelium is a hologram''&lt;nowiki&gt;; &lt;/nowiki&gt;'''Radical Mycology'''&lt;nowiki&gt;; Peter McCoy &lt;/nowiki&gt;Chthaeus Press ISBN 978-0-9863996-0-2<br /> <br /> '''² '''see page Growing Gourmet and Medicinal Mushrooms Paul Stamets</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:Habitats_SS18/Jan_Georg_Gl%C3%B6ckner&diff=97469 2018-06-06T11:14:43Z

<p>Jan Glöckner: </p> <hr /> <div>'''Life-Support-Systems'''<br /> <br /> <br /> '''Abstract'''<br /> <br /> <br /> I define systems that serve the purpose of sustaining live of the participating organisms as life-support-systems. This definition distinguishes life-support-systems from the term habitat, which is the natural environment of an organism, in which it can find food, shelter and reproduce. Life-support-systems are artifical and meant to stabilize the parameters of life of the participating organisms. This can include parameters from a natural enviromnent, maybe even mimicking it, but it is not the directive of a life-support-system. <br /> <br /> When an organisms wants to leave its natural habitat and colonize or travel to another habitat in which it cannot sustain its life-parameters, it needs a life-support-system. In fiction there are interesting examples of this bare necessity: from Dracula, who has to sleep in soil of his homeland, that he ships in coffins to London so he can sustain his life there, to the fremen-suits of the natives of Arakis, the desert planet in Frank Herbert‘s Dune, who use sophisticated wearables to survive in conditions of extreme heat and draught.<br /> <br /> My intention is to design a life-support-system that will sustain the life of a fungus for as long as possible. <br /> <br /> Based on the theory that a fungus will grow indefinetly, as long as it is provided with enough food and has no physical boundaries¹, I grow Pleurotus Ostreatus and Pleurotus Djamor in liquid culture. This theory opposes the opinion that fungi are subject to a process called senescence or biological ageing that leads to loss in vigor and the eventual death of a individuum².<br /> <br /> <br /> &lt;div style=&quot;color:#72bf44;&quot;&gt;'''Directive 1:'''&lt;/div&gt;<br /> <br /> <br /> Let the fungi grow in a shape that makes it easier for hominina to relate to it. The shape should be appealing and spark empathy.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 2:'''&lt;/div&gt;<br /> <br /> <br /> Investigate and search for partners to the fungi, that can supply the needed resources to sustain life.<br /> <br /> <br /> &lt;div style=&quot;color:#ce181e;&quot;&gt;'''Directive 3: '''&lt;/div&gt;<br /> <br /> <br /> Design a system that sustains the live of the fungi and their partner for as long as possible.<br /> <br /> <br /> &lt;div style=&quot;color:#ed1c24;&quot;&gt;Directive 4:&lt;/div&gt;<br /> <br /> Prove that there is no such thing as senescence for fungi and that it can be prevented in a laboratory set-up.<br /> <br /> <br /> '''Methodology'''<br /> <br /> <br /> Two fungi in the same genus Pleurotus ostreatus and djamor are cultivated in liquid culture. The formation of clusters is controlled by media formulation and by pressure of the aeriation-system.<br /> <br /> <br /> [[Image:Image1.png]]<br /> <br /> [[Image:Image2.png|top]]<br /> <br /> [[Image:Image3.png|top]]<br /> <br /> <br /> '''¹''' see page 206 ''Mycelium is a hologram''&lt;nowiki&gt;; &lt;/nowiki&gt;'''Radical Mycology'''&lt;nowiki&gt;; Peter McCoy &lt;/nowiki&gt;Chthaeus Press ISBN 978-0-9863996-0-2<br /> <br /> '''² '''see page Growing Gourmet and Medicinal Mushrooms Paul Stamets</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95792 2018-03-07T19:17:22Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations. As the genetic makeup of the strains change through cultivation, the creative commons licence is applied to the genome to keep it from being obscured by companies.<br /> <br /> <br /> [https://creativecommons.org/licenses/by-sa/4.0/ CC BY-SA 4.0] <br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95791 2018-03-07T19:16:49Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations. As the genetic makeup of the strains change through cultivation, the creative commons licence is applied to the genome to keep it from being obscured by companies.<br /> [https://creativecommons.org/licenses/by-sa/4.0/ CC BY-SA 4.0] <br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95790 2018-03-07T19:15:45Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations. As the genetic makeup of the strains change through cultivation, the creative commons licence is applied to the genome to keep it from being obscured by companies.[https://creativecommons.org/licenses/by-sa/4.0/ cc-by-sa-4.0] <br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95789 2018-03-07T19:14:28Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations. As the genetic makeup of the strains change through cultivation, the creative commons licence is applied to the genome to keep it from being obscured by companies.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95643 2018-02-23T13:49:58Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> <br /> <br /> <br /> &lt;u&gt;Oyster mushroom cloning:&lt;/u&gt;<br /> <br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> <br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> <br /> Inoculated 2x6 petris dishes with King oyster obtained from Rewe german origin<br /> <br /> and 2x6 with shiitake obtained from denns german origin ecological.<br /> <br /> Both specimen were sprayed with ethanol prior to taking the tissue samples.<br /> <br /> Incubation at 25°C.<br /> <br /> Prepared jars with silicon for Liquid Culture technique according to Hippie3.<br /> <br /> <br /> '''7. February'''<br /> <br /> <br /> sample-----growth-----contamination-----next action-----comments<br /> <br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> <br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> <br /> SAD3-------XX---------XX-------------------keep----------------------<br /> <br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> <br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> <br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> <br /> SBD3-------XXXXX---------------------------run-------------brown metabolite<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''1. February'''<br /> <br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> <br /> &lt;u&gt;Oyster mushroom cloning:&lt;/u&gt;<br /> <br /> <br /> sample------growth-----contamination-----comments<br /> <br /> SAD1--------------------XXX----------------------------<br /> <br /> SAD2--------X-----------X-------------------------------<br /> <br /> SAD3--------X-----------XX-----------------------------<br /> <br /> SAD4--------X-----------XX-----------------------------<br /> <br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> <br /> SBD2--------XX---------XX------------------------------<br /> <br /> SBD3--------XXX----------------------------------------<br /> <br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> <br /> &lt;u&gt;Oyster mushroom cloning:&lt;/u&gt;<br /> <br /> <br /> sample-----growth-----contamination-----comments<br /> <br /> SAD1----------------------------------------------------<br /> <br /> SAD2-------------------X--------------------------------<br /> <br /> SAD3-------X-----------XX------------------------------<br /> <br /> SAD4-------X-----------X-------------------------------<br /> <br /> SBD1-------XX---------X---------------------(leapoff)<br /> <br /> SBD2-------X-----------X-------------------------------<br /> <br /> SBD3-------X-------------------------------------------<br /> <br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> <br /> &lt;u&gt;Oyster mushroom cloning:&lt;/u&gt;<br /> <br /> <br /> sample---growth---contamination----<br /> <br /> &lt;nowiki&gt;-----------------------------------------&lt;/nowiki&gt;<br /> <br /> SAD1-----none-----none--------------<br /> <br /> SAD2-----none-----none--------------<br /> <br /> SAD3-----minor----none--------------<br /> <br /> SAD4-----none-----mild--------------<br /> <br /> SBD1-----very good----none---------<br /> <br /> SBD2----minor------mild-------------<br /> <br /> SBD3----good-------none<br /> <br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> <br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> <br /> E³ shows aggressive leap off.<br /> <br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> <br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> <br /> Transferred rhyzomorphic mycelium to E³.<br /> <br /> <br /> <br /> <br /> <br /> <br /> 23. January<br /> <br /> <br /> E² shows rhyzomorphic growth.<br /> <br /> Blanks are clean.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''18. January'''<br /> <br /> <br /> E² shows excellent leap of.<br /> <br /> Blanks are clean.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> <br /> Poured and sterilized plates. <br /> <br /> E² is growing rapidly.<br /> <br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> <br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> <br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> <br /> The Button Mushroom cloning:<br /> <br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> <br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> <br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> <br /> <br /> '''9. December'''<br /> <br /> <br /> Grain 2 Masters running! Marked G2B.<br /> <br /> G2A mycelium is getting a pinkish tone!<br /> <br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> <br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> <br /> <br /> '''8. December'''<br /> <br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> <br /> <br /> '''7. December'''<br /> <br /> <br /> sterilised the other 3G2<br /> <br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> <br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> <br /> <br /> '''6. December'''<br /> <br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> <br /> <br /> '''5.December'''<br /> <br /> <br /> G1 Masters are running.<br /> <br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''30. November'''<br /> <br /> <br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''26.November'''<br /> <br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> <br /> [[File:1590296.jpg|400px]]<br /> <br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycelish.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> <br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''16.November'''<br /> <br /> <br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> <br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> <br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> <br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> <br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> <br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> <br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> <br /> &lt;nowiki&gt;[[File:1590301.jpg|400px]]&lt;/nowiki&gt;<br /> <br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> <br /> Pleurotus growth becomes apparent. <br /> <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> <br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95642 2018-02-22T18:00:59Z

<p>Jan Glöckner: </p> <hr /> <div><br /> this wiki will be re-formatted in libreoffice---<br /> <br /> <br /> == '''22. February''' ==<br /> <br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> &lt;nowiki&gt;<br /> sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite<br /> <br /> &lt;/nowiki&gt;<br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95641 2018-02-22T17:58:47Z

<p>Jan Glöckner: </p> <hr /> <div><br /> == '''22. February''' ==<br /> <br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> &lt;nowiki&gt;<br /> sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite<br /> <br /> &lt;/nowiki&gt;<br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95640 2018-02-22T17:58:07Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> &lt;nowiki&gt;<br /> sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite<br /> <br /> &lt;/nowiki&gt;<br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95639 2018-02-22T17:57:44Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> <br /> &lt;nowiki&gt;&lt;nowiki&gt;sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite&lt;/nowiki&gt;&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95638 2018-02-22T17:57:21Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95637 2018-02-22T17:57:12Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> <br /> <br /> <br /> <br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95636 2018-02-22T17:56:43Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------&lt;/nowiki&gt;<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> &lt;nowiki&gt;sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------&lt;/nowiki&gt;<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95635 2018-02-22T17:55:39Z

<p>Jan Glöckner: </p> <hr /> <div>'''22. February'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> SB samples are vigorous in growth and show no contamination.<br /> SBD2 has been run on PDYA medium and shows most aggressive growth, it is growing on the top glass of the dish and breaking out. <br /> <br /> '''7. February'''<br /> <br /> sample-----growth-----contamination-----next action-----comments<br /> SAD1-------X-----------XXX-----------------discard--------------------<br /> SAD2-------XX---------XXXX----------------discard--------------------<br /> SAD3-------XX---------XX-------------------keep----------------------<br /> SAD4-------XXX-------XX--------------------keep---------------------<br /> SBD1-------XXXX------X---------------------run-------------brown metabolite<br /> SBD2-------XXXXX----XX--------------------run-------------brown metabolite<br /> SBD3-------XXXXX---------------------------run-------------brown metabolite<br /> <br /> <br /> <br /> '''1. February'''<br /> <br /> Moved Button mushroom storage slants to the fridge, as the wine cooler gets very hot when the sun is shinning on it.<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample------growth-----contamination-----comments<br /> SAD1--------------------XXX----------------------------<br /> SAD2--------X-----------X-------------------------------<br /> SAD3--------X-----------XX-----------------------------<br /> SAD4--------X-----------XX-----------------------------<br /> SBD1--------?-----------XXX-----------------unclear what is growing!<br /> SBD2--------XX---------XX------------------------------<br /> SBD3--------XXX----------------------------------------<br /> <br /> <br /> <br /> '''31. January'''<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample-----growth-----contamination-----comments<br /> SAD1----------------------------------------------------<br /> SAD2-------------------X--------------------------------<br /> SAD3-------X-----------XX------------------------------<br /> SAD4-------X-----------X-------------------------------<br /> SBD1-------XX---------X---------------------(leapoff)<br /> SBD2-------X-----------X-------------------------------<br /> SBD3-------X-------------------------------------------<br /> <br /> Placed Button Mushroom storage slants into cooling at 6°C.<br /> <br /> <br /> <br /> '''30. January'''<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> sample---growth---contamination----<br /> -----------------------------------------<br /> SAD1-----none-----none--------------<br /> SAD2-----none-----none--------------<br /> SAD3-----minor----none--------------<br /> SAD4-----none-----mild--------------<br /> SBD1-----very good----none---------<br /> SBD2----minor------mild-------------<br /> SBD3----good-------none<br /> <br /> the temperature in the laboratory has dropped under 20° C, as somebody left the windows open, the cultures were moved into the incubator at 25,5°C.<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner/The_digital_lab-book&diff=95634 2018-02-22T17:26:07Z

<p>Jan Glöckner: </p> <hr /> <div>30. January<br /> <br /> One of the cold storage slants for brown button mushroom has developed a lustrous surface coating. The other slant is close to fully covering the surface,<br /> <br /> Oyster mushroom cloning:<br /> <br /> <br /> <br /> '''28. January'''<br /> <br /> Cloned 2 specimen of oyster mushrooms obtained from globus supermarket. The mushrooms are produced in Poland.<br /> 4 dishes were inoculated with specimen A. 3 dishes with specimen B. They are incubated at room temperature ( 23°C)<br /> <br /> autoclaved the horrible jar of penicilium.<br /> <br /> <br /> <br /> '''26. January'''<br /> <br /> E³ shows aggressive leap off.<br /> <br /> Inoculated two long-storage-slants with the Brown Button Mushroom mycelium (E²). <br /> <br /> The old Pleurotus culture on cardboard gives mushrooms.<br /> Moved it from cold storage to the windowsill.<br /> <br /> <br /> <br /> '''24. January'''<br /> <br /> Transferred rhyzomorph mycelium to E³.<br /> <br /> <br /> <br /> '''23. January'''<br /> <br /> E² shows rhyzomorph growth.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''18. January''' <br /> <br /> E² shows excellent leap of.<br /> Blanks are clean.<br /> <br /> <br /> <br /> '''14. January'''<br /> <br /> Poured and sterilized plates. <br /> E² is growing rapidly.<br /> <br /> Two blanks are incubated to see if there is any contamination introduced through bad sterile-technique.<br /> <br /> <br /> <br /> '''12. January'''<br /> <br /> Due to long illness all cultures are neglected:<br /> <br /> -all Grain-masters are over-incubated, None is usable for further development.<br /> Through poor air-circulation (no shaking) anaerobic conditions prevented the mycelium running. The mycelium itself looks healthy, but green molds are sharing the habitat.<br /> The mycelium ranges in colour from white over purple to red. <br /> <br /> The Button Mushroom cloning:<br /> <br /> all dishes save E, are contaminated and the mycelium has died.<br /> E sports cottony mycelium and it seems like primordia forming, there is no contamination.<br /> The mycelium from E is transferred to another dish labelled E².<br /> <br /> <br /> <br /> '''13. December'''<br /> <br /> Button Mushroom cloning. dishes showing mycelial leap of on the medium: A. B. C. D. E. Dishes showing no myceliuml growth whatsoever: G.<br /> <br /> <br /> <br /> [[File:G2running.jpg|400px]]<br /> <br /> the picture was taken on the 13th. (the date above the line should read 9.12 not 9.11) After the picture was taken I shock all G2 Masters. One of the G2A jarls lost its professional filter cotton ball. I employed questionable practices and replaced the cotton ball after extraction under the laminar flow hood, with a new one I soaked in ethanol. I separated the jar from the others and marked it with a red circle.<br /> <br /> <br /> '''9. December'''<br /> <br /> Grain 2 Masters running! Marked G2B.<br /> G2A mycelium is getting a pinkish tone!<br /> <br /> Inoculated 6 Potato Dextrose Agar dishes with Brown Button Mushroom<br /> Method: cloning. Strain: commercial, Poland ( from the supermarket)<br /> <br /> <br /> '''8. December'''<br /> <br /> Inoculated 3 G2B.<br /> <br /> <br /> '''7. December'''<br /> <br /> sterilised the other 3G2<br /> Two Pleurotus samples were dried out in their dishes in an attempt to let them form primordia, with they did, until they where hard and dead.<br /> They might be of value to Suna, and her paper project, I keep them.<br /> <br /> <br /> '''6. December'''<br /> <br /> sterilised 3 of the G2 and inoculated with G1A.<br /> <br /> <br /> [[File:stirrer.MOV]]<br /> <br /> I built an magnetic stirrer. It runs on 5 volts. Should be sufficient for now.<br /> <br /> <br /> '''5.December'''<br /> <br /> G1 Masters are running.<br /> Autoclaving dark mutants and empty kimchi jar.<br /> <br /> Prepared six G2 bottles according to Stamets (soaking overnight to germinate endospores)<br /> <br /> <br /> <br /> '''3. December'''<br /> <br /> Placed one of the G1 Masters in the incubator at 25°C.<br /> <br /> <br /> <br /> '''1. December'''<br /> <br /> Inoculated Grain Master G1. (2x 150g)<br /> <br /> <br /> <br /> '''30. November'''<br /> Prepared rye for grain master according to Stamets.<br /> <br /> <br /> <br /> '''29. November'''<br /> <br /> The kimchi jar shows no signs of growth, nor contamination. Nothing shows up under the microscope.<br /> <br /> <br /> '''<br /> 26.November'''<br /> <br /> All Pleurotus samples look very promising. The F1 generation samples are thriving. No contamination at all. Very soon the production of spawn can begin. The peg sample is now all cottony mycelium. So no yeasts, just the look of them. Maybe there are more of this early stage structures in the sample, or it is minor contamination. The control blank shows no sign of contamination. <br /> <br /> [[File:1590297.jpg|400px]] [[File:1590300.jpg|400px]]<br /> <br /> Inoculated on jar of LA-bacteria-medium with kirby and cabbage kimchi samples.<br /> <br /> <br /> <br /> '''19. November'''<br /> <br /> Pleurotus: all samples show good growth and no contamination. Cottony mycelium (typical for pleurotus ostreatus) is developing.<br /> [[File:1590296.jpg|400px]]<br /> <br /> The peg jar looks strange though. I rolled the peg around in the dish on the 17th. Now the surface of the medium shows the tracks of this action. But the developing organism looks rather yeasty than mycely. <br /> <br /> <br /> <br /> '''17. November'''<br /> <br /> sterilised and inoculated 3 petri dishes with Pleurotus strains from the peg on malt-medium. Transferred the original peg to one petri dish and kept one blank for controlling. All dishes are Potato-Dextrose-Agar-Medium.<br /> the tupperware pleurotus caught on in growing, but still is very restrained.<br /> <br /> <br /> <br /> '''16.November'''<br /> Cooked and sterilised 1 Liter of Potato-Dextrose-Agar-Medium. Poured 5 petri-dishes, to be sterilised on friday.<br /> Still no luminescence in vibr. phosp.<br /> <br /> <br /> <br /> '''15. November'''<br /> <br /> Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.<br /> the tupperware sample still is, if growing very slowly but shows no signs of contamination.<br /> the temperature of the incubator is now 25.5°C<br /> <br /> <br /> <br /> '''9. November'''<br /> <br /> Discarded jar A of vibrio phosp. due to high contamination.<br /> transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)<br /> <br /> <br /> <br /> '''8. November'''<br /> <br /> vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.<br /> still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''<br /> Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.<br /> <br /> [[File:1590301.jpg|400px]]<br /> <br /> supplementary: vibrio phosp. opened jar A and shock it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effect. the sample has to be discarded anyway.<br /> <br /> <br /> <br /> '''2.November'''<br /> <br /> Pleurotus growth becomes apparent. <br /> vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''<br /> <br /> Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.<br /> <br /> <br /> <br /> '''1.November'''<br /> <br /> Autoclaved two dishes filled with LA-Medium, one of the dishes filled up with water during the process, discarded and poured with leftover medium that has been in the autoclave, and inoculated with vibrio phosphoreum around 16.00.<br /> they should start to glow in 12-24 hours.<br /> <br /> <br /> <br /> '''31. October'''<br /> <br /> starting to grow vibrio phosphoreum from a batch that was labelled Photobacterium phosp. and seemed to be inoculated somewhere in 2016. (the writing on the petri-dish was rendered unreadable)<br /> <br /> cooked 100ml of LA-Medium and left it overnight in the fridge.<br /> <br /> ''on plastic petri-dishes: I decided to use only glass-petri-dishes, as they give me the opportunity to sterilise medium inside the dishes, thus limiting to chances for contamination greatly. and they produce less waste, are to be recycled if broken, if not broken have a unlimited shelf life and usage time.''<br /> <br /> migrated the pleurotus from the plastic mushroom supermarket dish to a tupperware. no sign of growth.<br /> <br /> <br /> <br /> '''25. October'''<br /> <br /> inoculated cooked cardboard with Pleurotus pegs.</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95616 2018-02-19T15:29:20Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium. I then distribute the organism, for everyone to culture and distribute, in a respectful way and to form mutual diplomatic relations.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95615 2018-02-16T02:07:29Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 1,2 and stage 3 [petri dishes A to D and G.H not shown] ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium, and distribute the organism, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95614 2018-02-16T02:04:39Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 2 and stage 3 ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products, by reverse engineering the product to its viable state of mycelium, and distribute the organism, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95613 2018-02-16T02:00:32Z

<p>Jan Glöckner: </p> <hr /> <div>[[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]<br /> <br /> <br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 2 and stage 3 ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products and distribute the organism in the form of viable mycelium, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95612 2018-02-16T01:59:03Z

<p>Jan Glöckner: </p> <hr /> <div><br /> '''supermarket piracy programm'''<br /> <br /> Oyster mushroom cloning series stage 1 (obtained from Globus supermarket; polish origin)<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> Brown Button mushroom cloning series stage 2 and stage 3 ( obtained from Rewe supermarket; german origin)<br /> &lt;gallery&gt;<br /> File:button E1 alternateview.JPG<br /> File:button E2.JPG<br /> File:button E3.JPG<br /> &lt;/gallery&gt;<br /> <br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products and distribute the organism in the form of viable mycelium, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7<br /> <br /> [[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Button_E3.JPG&diff=95611 2018-02-16T01:56:31Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Button_E2.JPG&diff=95610 2018-02-16T01:55:59Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Button_E1_alternateview.JPG&diff=95609 2018-02-16T01:55:24Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95608 2018-02-16T01:50:58Z

<p>Jan Glöckner: </p> <hr /> <div><br /> '''supermarket piracy programm'''<br /> &lt;gallery&gt;<br /> File:Pleur.ostr.SAD1.JPG<br /> File:Pleur.ostr.SAD1 alternateview.JPG<br /> File:Pleur.ostr.SAD2.JPG<br /> File:Pleur.ostr.SAD3.JPG<br /> File:Pleur.ostr.SAD4.JPG<br /> File:Pleur.ostr.SBD1.JPG<br /> File:Pleur.ostr.SBD2.JPG<br /> File:Pleur.ostr.SBD3.JPG<br /> File:Pleur.ostr.SBD3 alternateview.JPG<br /> &lt;/gallery&gt;<br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products and distribute the organism in the form of viable mycelium, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7<br /> <br /> [[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SBD3_alternateview.JPG&diff=95607 2018-02-16T01:50:09Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SBD3.JPG&diff=95606 2018-02-16T01:49:35Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SBD2.JPG&diff=95605 2018-02-16T01:48:57Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SBD1.JPG&diff=95604 2018-02-16T01:48:25Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SAD4.JPG&diff=95603 2018-02-16T01:47:52Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SAD3.JPG&diff=95602 2018-02-16T01:47:22Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SAD2.JPG&diff=95601 2018-02-16T01:46:52Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SAD1_alternateview.JPG&diff=95600 2018-02-16T01:46:21Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:Pleur.ostr.SAD1.JPG&diff=95599 2018-02-16T01:45:53Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95598 2018-02-16T01:34:17Z

<p>Jan Glöckner: </p> <hr /> <div><br /> '''supermarket piracy programm'''<br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products and distribute the organism in the form of viable mycelium, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7<br /> <br /> [[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95597 2018-02-16T01:32:32Z

<p>Jan Glöckner: </p> <hr /> <div><br /> '''supermarket piracy programm'''<br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products and distribute the organism in the form of viable mycelium, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7<br /> &lt;gallery&gt;<br /> File:2018_02_07_0001.JPG<br /> File:2018_02_07_0002.JPG<br /> File:2018_02_07_0004.JPG<br /> File:2018_02_07_0005.JPG<br /> File:2018_02_07_0006.JPG<br /> File:2018_02_07_0007.JPG<br /> File:2018_02_07_0008.JPG text here<br /> File:2018_02_07_0009.JPG<br /> File:2018_02_07_0010.JPG<br /> File:2018_02_07_0011.JPG<br /> File:2018_02_07_0013.JPG<br /> File:2018_02_07_0014.JPG<br /> File:2018_02_07_0015.JPG<br /> File:2018_02_07_0016.JPG<br /> File:2018_02_07_0017.JPG<br /> &lt;gallery&gt;<br /> <br /> [[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0001.JPG&diff=95596 2018-02-16T01:23:15Z

<p>Jan Glöckner: Jan Glöckner uploaded a new version of File:2018 02 07 0001.JPG</p> <hr /> <div></div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0001.JPG&diff=95595 2018-02-16T01:22:20Z

<p>Jan Glöckner: Blanked the page</p> <hr /> <div></div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0001.JPG&diff=95594 2018-02-16T01:20:31Z

<p>Jan Glöckner: Jan Glöckner uploaded a new version of File:2018 02 07 0001.JPG</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY_Biolab_%E2%80%9CDriver%E2%80%99s_License%E2%80%9D/Jan_Georg_Gl%C3%B6ckner&diff=95593 2018-02-16T01:15:28Z

<p>Jan Glöckner: </p> <hr /> <div><br /> '''supermarket piracy programm'''<br /> <br /> Artistic Abstract<br /> <br /> To address the growing issue of patented living organisms, I pirate the genome of store-bought mushroom-products and distribute the organism in the form of viable mycelium, for everyone to grow and distribute the organism, in a respectful way.<br /> <br /> Scientific Abstract<br /> <br /> Specimen of mushrooms obtained from supermarkets are cloned and the developing strain is purified, than stored in a cold-storage strain-bank. Via reverse-engineering the strain from a commercial product is extracted and cultured. The strains will be given away, and kept available.<br /> <br /> Method<br /> <br /> For every attempt of cloning at least seven petri-dishes filled with PDA- medium are inoculated. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, inside the stem or just under the cap. It is inserted in the hole that the hot scalpel made. The dishes are labeled and incubated in the incubator (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till there is no visible evidence of contaminants. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strain, enveloped in a zip-loc-bag. ( liquid suspended-animation technique will be introduced.)<br /> <br /> References<br /> <br /> Paul Stamets and J.S. Chilton: The Mushroom Cultivator; Sterile Technique and Agar Culture: Starting a Culture From Live Tissue (p. 29) 1983 Agarikon Press Olympia Washington ISBN: 978-0-9610798-0-2 Paul Stamets: Growing Gourmet and Medicinal Mushrooms (Third Edition); Culturing Mushroom Mycelium on Agar Media: Starting a Mushroom Strain by Cloning (p. 88); 2000 Ten Speed Press ( Random House New York) ISBN: 978-1-58008-175-7<br /> &lt;gallery&gt;<br /> File:2018_02_07_0001.JPG<br /> File:2018_02_07_0002.JPG<br /> File:2018_02_07_0004.JPG<br /> File:2018_02_07_0005.JPG<br /> File:2018_02_07_0006.JPG<br /> File:2018_02_07_0007.JPG<br /> File:2018_02_07_0008.JPG<br /> File:2018_02_07_0009.JPG<br /> File:2018_02_07_0010.JPG<br /> File:2018_02_07_0011.JPG<br /> File:2018_02_07_0013.JPG<br /> File:2018_02_07_0014.JPG<br /> File:2018_02_07_0015.JPG<br /> File:2018_02_07_0016.JPG<br /> File:2018_02_07_0017.JPG<br /> &lt;/gallery&gt;<br /> <br /> [[/The digital lab-book|The digital lab-book]] ( this is the unedited version of the lab-book, that will be the most up-to-date document)<br /> <br /> <br /> [[/General methods|General methods]]</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0018.JPG&diff=95592 2018-02-16T01:10:06Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0017.JPG&diff=95591 2018-02-16T01:09:27Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0016.JPG&diff=95590 2018-02-16T01:08:55Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0015.JPG&diff=95589 2018-02-16T01:08:20Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0014.JPG&diff=95588 2018-02-16T01:07:48Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=File:2018_02_07_0013.JPG&diff=95587 2018-02-16T01:07:13Z

<p>Jan Glöckner: File uploaded with MsUpload</p> <hr /> <div>File uploaded with MsUpload</div>

Jan Glöckner