Medien Wiki - User contributions [en]

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:51:16Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link: [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:50:29Z

Goji6184:

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:50:13Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link: [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:''
https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:49:07Z

Goji6184: /* PROJECT IDEA 1 */

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:48:50Z

Goji6184: /* PROJECT IDEA 1 */

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link:

[https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/Project: Specimen ''Physarum Polycephalum''.

2019-04-03T10:47:53Z

Goji6184: Created page with "== ''' Physarum Polycephalum.''' == '''Project idea.''' The original idea was to see if there was any relation between Alii Vibrio fischeri (a specimen that life in warm ar..."

== ''' Physarum Polycephalum.''' ==

'''Project idea.'''

The original idea was to see if there was any relation between Alii Vibrio fischeri (a specimen that life in warm around 20°C oceans) & Physarum Polycephalum (a specimen that life's in the forest in warm temperatures 20°C). In order to test this, I made an investigation around the two specimens. The second step was to cultivate both of them, sadly the extraction of the first specimen from an octopus didn't give results, so I redirected my project into teaching Physarum Polycephalum to adapt to salinity environments.

[[File:52h salinity medium.mp4|1500px]]

Here can be seen a video around how specimens that were teached to live in different salinity mediums behave when they have the possibility to move between different salinity mediums (Between 0% salinity and 0.5% salinity). It is a 52 h photage of Physarum Polycephalum in salinity medium.

'''Investigation around the specimens.'''

In the following links can be found the investigation around the two specimens, the medium that they need and more.

[[/Investigation around Aliivibrio Fischeri.]]

[[/Investigation around Physarum Polycephalum.]]

'''Specimens Mediums.'''

- Medium A.F:
*NaCl 30 g
*Glycerol 1 g
*Pepton (Bacxto-peptone) 10 g
*Meat extract 3 g
*Made up with distilled water to 1000 ml

- Medium P.P:
*100ml of distilled water
*2g of agar
*Leaflets

'''Experimentation with the specimens.'''

In the following links can be found the diary of work made with each specimen, the experiments that were made with each of them, the experimentation with some mediums and more.

[[/Diary of work: Alii Vibrio Fischeri]]

[[/Diary of work: Physarum Polycephalum]]

'''Conclusions:'''

After making the salinity experiment with the Physarum Polycephalum, I get into the conclusion that this specimen can learn to survive in mediums that have salt concentrations. It can learn to survive in them in a process of increasing the salinity, but not if it is situated in a salinity medium without previous teaching.

After the experiment comes to a lot of questions that can be solved with further experiments like; 1) how fast does it forget? 2) Does it remember after a period of sleep? 3) Is the sclerotium cultivation possible after a period of time?, 4) the specimen showed troubles to find the food in high levels of salinity, Does the levels of NaCl affect the ability of the specimen to find the food?

''Salinity Percentage Mediums: ''Strains P.p.P''
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

During the time that I adopted this specimen, I made many tests in order to get to know and understand the specimen. I'm also taking the class of "DIY Bio; doing things with biology", where I made many tests in terms of food, temperature, and light.

This information can be found on the following website along with information of other experimentation:

- https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Paola_Stephania_Calder%C3%B3n

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:47:24Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU: Experimental Microbiology, the experiment, and results can be seen in the following link:

[[/Project: Specimen ''Physarum Polycephalum''.]]

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:46:13Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

[[[/Project: Specimen ''Physarum Polycephalum''.]]]

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:44:52Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.7%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:41:46Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

The initial approximation to the specimen was made in the class: ''DIY BIO: doing things with biology''. In this class three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:39:33Z

Goji6184:

==INTRODUCCTION==

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''. During this class, I developed other experiments that build my knowledge around the work inside the laboratory, the cultivation of different kinds of specimens and techniques around the lab. work.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:35:10Z

Goji6184:

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project idea 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project idea 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-03T10:33:47Z

Goji6184:

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made two projects. The '''project number 1: ''(teaching Physarum Polycephalum to live in salinity mediums)'' ''' is the result of the knowledge that was acquired around the selected specimen ''Physarum Polycephalum'' in the class ''DIY: Bio: doing things with biology'' and the experimentation made in the class ''Experimental microbiology''. The '''project number 2: ''(insolation of gluconacetobacter with coral shape)'' ''' is the result of the knowledge build during the class ''DIY: Bio: doing things with biology''.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-02T13:28:50Z

Goji6184:

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made a project (project number 1: teaching Physarum Polycephalum to live in salinity mediums) that involves the knowledge acquired in this class and in the class.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-04-02T13:27:30Z

Goji6184:

In this class, I develop the basic knowledge around the work inside a laboratory, I acquired different kinds of knowledge around different specimens that were adopted and as a final result, I made a project (project number 1) that involves the knowledge acquired in this class and in another class.

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:34:43Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

File:Gluconacetobacter guide.jpg

2019-03-28T08:34:25Z

Goji6184: File uploaded with MsUpload

File uploaded with MsUpload

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:33:50Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

File:Gluconacetobacter lab guide.jpg

2019-03-28T08:33:37Z

Goji6184: Goji6184 uploaded a new version of File:Gluconacetobacter lab guide.jpg

File uploaded with MsUpload

File:Gluconacetobacter lab guide.jpg

2019-03-28T08:32:59Z

Goji6184: Goji6184 uploaded a new version of File:Gluconacetobacter lab guide.jpg

File uploaded with MsUpload

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:23:36Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:23:05Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:22:46Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''

https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:21:50Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:
https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.

''All experiments made in the class "Experimental Microbiology" can be seen at:''
https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

Paola S. Calderón/Project: Specimen ''Physarum Polycephalum''.

2019-03-28T08:20:25Z

Goji6184:

Paola S. Calderón/Project: Specimen ''Physarum Polycephalum''.

2019-03-28T08:19:29Z

Goji6184:

== ''' Physarum Polycephalum.''' ==

'''Project idea.'''

The original idea was to see if there was any relation between Alii Vibrio fischeri (a specimen that life in warm around 20°C oceans) & Physarum Polycephalum (a specimen that life's in the forest in warm temperatures 20°C). In order to test this, I made an investigation around the two specimens. The second step was to cultivate both of them, sadly the extraction of the first specimen from an octopus didn't give results, so I redirected my project into teaching Physarum Polycephalum to adapt to salinity environments.

[[File:52h salinity medium.mp4|1500px]]

Here can be seen a video around how specimens that were teached to live in different salinity mediums behave when they have the possibility to move between different salinity mediums (Between 0% salinity and 0.5% salinity). It is a 52 h photage of Physarum Polycephalum in salinity medium.

'''Investigation around the specimens.'''

In the following links can be found the investigation around the two specimens, the medium that they need and more.

[[/Investigation around Aliivibrio Fischeri.]]

[[/Investigation around Physarum Polycephalum.]]

'''Specimens Mediums.'''

- Medium A.F:
*NaCl 30 g
*Glycerol 1 g
*Pepton (Bacxto-peptone) 10 g
*Meat extract 3 g
*Made up with distilled water to 1000 ml

- Medium P.P:
*100ml of distilled water
*2g of agar
*Leaflets

'''Experimentation with the specimens.'''

In the following links can be found the diary of work made with each specimen, the experiments that were made with each of them, the experimentation with some mediums and more.

[[/Diary of work: Alii Vibrio Fischeri]]

[[/Diary of work: Physarum Polycephalum]]

'''Conclusions:'''

After making the salinity experiment with the Physarum Polycephalum, I get into the conclusion that this specimen can learn to survive in mediums that have salt concentrations. It can learn to survive in them in a process of increasing the salinity, but not if it is situated in a salinity medium without previous teaching.

After the experiment comes to a lot of questions that can be solved with further experiments like; 1) how fast does it forget? 2) Does it remember after a period of sleep? 3) Is the sclerotium cultivation possible after a period of time?, 4) the specimen showed troubles to find the food in high levels of salinity, Does the levels of NaCl affect the ability of the specimen to find the food?

''Salinity Percentage Mediums: ''Strains P.p.P''
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

During the time that I adopted this specimen, I made many tests in order to get to know and understand the specimen. I'm also taking the class of "DIY Bio; doing things with biology", where I made many tests in terms of food, temperature, and light. This information can be found on the following website:

- https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Paola_Stephania_Calder%C3%B3n

Paola S. Calderón

2019-03-28T08:15:21Z

Goji6184:

'''BACKGROUND.'''

*Architect ''(2012)'' and Designer with an emphasis in product design ''(2013)'' of Los Andes University (Bogotá/Colombia)
*Currently making Master in Media Architecture in Bauhaus University (Weimar/Germany) ''(2018-2020)''

'''PERSONAL REASONS TO ATTENDING THE COURSE'''

As an architect and a product designer, the scale with I interact in my everyday life is a macro scale, that is based in KM for urbanistic projects, M for building, CM and MM for product design. The scale of the microbiology world is something that I have always find fascinating, but sadly since the school, I haven't had the opportunity to interact with it. This course offers me the opportunity to reconnect with this world, to learn from it, experiment and the opportunity to see it through different lenses. I hope that at the end of this course I will be able to understand what experimental microbiology is and see if this subject has a connection to my previous knowledge.

'''LINKS TO ORGANISM ADOPTION'''

[[File:DATA sheet Physarum Polycephalum.jpg|600px]]

[[/Project: Specimen ''Physarum Polycephalum''.]]
Is a protist known as the “many-headed slime”. It has a characteristic strong yellow color. It uses cytoplasmic streaming to search for food, the logic behind this specimen’s movement is still a subject of study for biologist and scientists.

[[File:DATA sheet Haematoccocus Pluvialis.jpg|600px]]

[[/Specimen adoption 1: ''Haematococcus Pluvialis''.]]
Is a green alga with mixotrophic growth. It has chlorophyll a and b and has the ability to synthesize carotenoids such as Astaxanthin that can accumulate in a proportion of even 5% of its dry weight.

[[File:DATA sheet Armillaria Gallica.jpg|600px]]

[[/Specimen adoption 2: ''Armillaria Gallica''.]]
Is a fungi that live in the forest, have a root structure around of 2mm diameter. It likes dark and warm environments. Its roots are white while it is growing and acquire a dark brown color after it stops growing.

'''LINKS WORKSHOP AND PREVIOUS INVESTIGATION'''

[[/PREVIOUS-INVESTIGATION.]]
In order to decide which microorganism are suitable for the work that is going to be developed, an initial investigation is made in order to understand the characteristics of each microorganism, their needs in order to grow, and important information suitable for the project.

[[/WORKSHOP]]
During the three first days, a series of activities were made, and are described in the link.

Paola S. Calderón/Project: Specimen ''Physarum Polycephalum''.

2019-03-28T08:15:03Z

Goji6184: /* Physarum Polycephalum. */

== ''' Physarum Polycephalum.''' ==

'''Project idea.'''

The original idea was to see if there was any relation between Alii Vibrio fischeri (a specimen that life in warm around 20°C oceans) & Physarum Polycephalum (a specimen that life's in the forest in warm temperatures 20°C). In order to test this, I made an investigation around the two specimens. The second step was to cultivate both of them, sadly the extraction of the first specimen from an octopus didn't give results, so I redirected my project into teaching Physarum Polycephalum to adapt to salinity environments.

[[File:52h salinity medium.mp4|1500px]]

Here can be seen a video around how specimens that were teached to live in different salinity mediums behave when they have the possibility to move between different salinity mediums (Between 0% salinity and 0.5% salinity). It is a 52 h photage of Physarum Polycephalum in salinity medium.

'''Investigation around the specimens.'''

In the following links can be found the investigation around the two specimens, the medium that they need and more.

[[/Investigation around Aliivibrio Fischeri.]]

[[/Investigation around Physarum Polycephalum.]]

'''Specimens Mediums.'''

- Medium A.F:
*NaCl 30 g
*Glycerol 1 g
*Pepton (Bacxto-peptone) 10 g
*Meat extract 3 g
*Made up with distilled water to 1000 ml

- Medium P.P:
*100ml of distilled water
*2g of agar
*Leaflets

'''Experimentation with the specimens.'''

In the following links can be found the diary of work made with each specimen, the experiments that were made with each of them, the experimentation with some mediums and more.

[[/Diary of work: Alii Vibrio Fischeri]]

[[/Diary of work: Physarum Polycephalum]]

'''Conclusions:'''

After making the salinity experiment with the Physarum Polycephalum, I get into the conclusion that this specimen can learn to survive in mediums that have salt concentrations. It can learn to survive in them in a process of increasing the salinity, but not if it is situated in a salinity medium without previous teaching.

After the experiment comes to a lot of questions that can be solved with further experiments like; 1) how fast does it forget? 2) Does it remember after a period of sleep? 3) Is the sclerotium cultivation possible after a period of time?, 4) the specimen showed troubles to find the food in high levels of salinity, Does the levels of NaCl affect the ability of the specimen to find the food?

''Salinity Percentage Mediums: ''Strains P.p.P''
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

During the time that I adopted this specimen, I made many tests in order to get to know and understand the specimen. I'm also taking the class of "DIY Bio; doing things with biology", where I made many tests in terms of food, temperature, and light. This information can be found on the following websites:

- https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Paola_Stephania_Calder%C3%B3n/23.10.2018

-https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Paola_Stephania_Calder%C3%B3n/20.11.2018

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:14:36Z

Goji6184: /* PROJECT IDEA 1 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
File:1% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

File:1% salinity.png

2019-03-28T08:14:21Z

Goji6184: File uploaded with MsUpload

File uploaded with MsUpload

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-28T08:13:18Z

Goji6184: /* PROJECT IDEA 1 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

Paola S. Calderón

2019-03-28T00:01:19Z

Goji6184:

'''BACKGROUND.'''

*Architect ''(2012)'' and Designer with an emphasis in product design ''(2013)'' of Los Andes University (Bogotá/Colombia)
*Currently making Master in Media Architecture in Bauhaus University (Weimar/Germany) ''(2018-2020)''

'''PERSONAL REASONS TO ATTENDING THE COURSE'''

As an architect and a product designer, the scale with I interact in my everyday life is a macro scale, that is based in KM for urbanistic projects, M for building, CM and MM for product design. The scale of the microbiology world is something that I have always find fascinating, but sadly since the school, I haven't had the opportunity to interact with it. This course offers me the opportunity to reconnect with this world, to learn from it, experiment and the opportunity to see it through different lenses. I hope that at the end of this course I will be able to understand what experimental microbiology is and see if this subject has a connection to my previous knowledge.

'''LINKS TO ORGANISM ADOPTION'''

[[File:DATA sheet Physarum Polycephalum.jpg|700px]]

[[/Project: Specimen ''Physarum Polycephalum''.]]
Is a protist known as the “many-headed slime”. It has a characteristic strong yellow color. It uses cytoplasmic streaming to search for food, the logic behind this specimen’s movement is still a subject of study for biologist and scientists.

[[File:DATA sheet Haematoccocus Pluvialis.jpg|700px]]

[[/Specimen adoption 1: ''Haematococcus Pluvialis''.]]
Is a green alga with mixotrophic growth. It has chlorophyll a and b and has the ability to synthesize carotenoids such as Astaxanthin that can accumulate in a proportion of even 5% of its dry weight.

[[File:DATA sheet Armillaria Gallica.jpg|700px]]

[[/Specimen adoption 2: ''Armillaria Gallica''.]]
Is a fungi that live in the forest, have a root structure around of 2mm diameter. It likes dark and warm environments. Its roots are white while it is growing and acquire a dark brown color after it stops growing.

'''LINKS WORKSHOP AND PREVIOUS INVESTIGATION'''

[[/PREVIOUS-INVESTIGATION.]]
In order to decide which microorganism are suitable for the work that is going to be developed, an initial investigation is made in order to understand the characteristics of each microorganism, their needs in order to grow, and important information suitable for the project.

[[/WORKSHOP]]
During the three first days, a series of activities were made, and are described in the link.

Paola S. Calderón

2019-03-28T00:00:53Z

Goji6184:

'''BACKGROUND.'''

*Architect ''(2012)'' and Designer with an emphasis in product design ''(2013)'' of Los Andes University (Bogotá/Colombia)
*Currently making Master in Media Architecture in Bauhaus University (Weimar/Germany) ''(2018-2020)''

'''PERSONAL REASONS TO ATTENDING THE COURSE'''

As an architect and a product designer, the scale with I interact in my everyday life is a macro scale, that is based in KM for urbanistic projects, M for building, CM and MM for product design. The scale of the microbiology world is something that I have always find fascinating, but sadly since the school, I haven't had the opportunity to interact with it. This course offers me the opportunity to reconnect with this world, to learn from it, experiment and the opportunity to see it through different lenses. I hope that at the end of this course I will be able to understand what experimental microbiology is and see if this subject has a connection to my previous knowledge.

'''LINKS TO ORGANISM ADOPTION'''

[[File:DATA sheet Physarum Polycephalum.jpg|700px]]

[[/Project: Specimen ''Physarum Polycephalum''.]]
Is a protist known as the “many-headed slime”. It has a characteristic strong yellow color. It uses cytoplasmic streaming to search for food, the logic behind this specimen’s movement is still a subject of study for biologist and scientists.

[[File:DATA sheet Haematoccocus Pluvialis.jpg|700px]]

[[/Specimen adoption 1: ''Haematococcus Pluvialis''.]]
Is a green alga with mixotrophic growth. It has chlorophyll a and b and has the ability to synthesize carotenoids such as Astaxanthin that can accumulate in a proportion of even 5% of its dry weight.

[[File:DATA sheet Armillaria Gallica.jpg|700px]]

[[/Specimen adoption 2: ''Armillaria Gallica''.]]
Is a fungi that live in the forest, have a root structure around of 2mm diameter. It likes dark and warm environments. Its roots are white while it is growing and acquire a dark brown color after it stops growing.

'''LINKS WORKSHOP AND PREVIOUS INVESTIGATION'''

[[/PREVIOUS-INVESTIGATION.]]
In order to decide which microorganism are suitable for the work that is going to be developed, an initial investigation is made in order to understand the characteristics of each microorganism, their needs in order to grow, and important information suitable for the project.

[[/WORKSHOP]]
During the three first days, a series of activities were made, and are described in the link.

Paola S. Calderón

2019-03-28T00:00:34Z

Goji6184:

'''BACKGROUND.'''

*Architect ''(2012)'' and Designer with an emphasis in product design ''(2013)'' of Los Andes University (Bogotá/Colombia)
*Currently making Master in Media Architecture in Bauhaus University (Weimar/Germany) ''(2018-2020)''

'''PERSONAL REASONS TO ATTENDING THE COURSE'''

As an architect and a product designer, the scale with I interact in my everyday life is a macro scale, that is based in KM for urbanistic projects, M for building, CM and MM for product design. The scale of the microbiology world is something that I have always find fascinating, but sadly since the school, I haven't had the opportunity to interact with it. This course offers me the opportunity to reconnect with this world, to learn from it, experiment and the opportunity to see it through different lenses. I hope that at the end of this course I will be able to understand what experimental microbiology is and see if this subject has a connection to my previous knowledge.

'''LINKS TO ORGANISM ADOPTION'''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

[[/Project: Specimen ''Physarum Polycephalum''.]]
Is a protist known as the “many-headed slime”. It has a characteristic strong yellow color. It uses cytoplasmic streaming to search for food, the logic behind this specimen’s movement is still a subject of study for biologist and scientists.

[[File:DATA sheet Haematoccocus Pluvialis.jpg|800px]]

[[/Specimen adoption 1: ''Haematococcus Pluvialis''.]]
Is a green alga with mixotrophic growth. It has chlorophyll a and b and has the ability to synthesize carotenoids such as Astaxanthin that can accumulate in a proportion of even 5% of its dry weight.

[[File:DATA sheet Armillaria Gallica.jpg|800px]]

[[/Specimen adoption 2: ''Armillaria Gallica''.]]
Is a fungi that live in the forest, have a root structure around of 2mm diameter. It likes dark and warm environments. Its roots are white while it is growing and acquire a dark brown color after it stops growing.

'''LINKS WORKSHOP AND PREVIOUS INVESTIGATION'''

[[/PREVIOUS-INVESTIGATION.]]
In order to decide which microorganism are suitable for the work that is going to be developed, an initial investigation is made in order to understand the characteristics of each microorganism, their needs in order to grow, and important information suitable for the project.

[[/WORKSHOP]]
During the three first days, a series of activities were made, and are described in the link.

Paola S. Calderón/Project: Specimen ''Physarum Polycephalum''.

2019-03-27T23:59:50Z

Goji6184: /* Physarum Polycephalum. */

== ''' Physarum Polycephalum.''' ==

'''Project idea.'''

The original idea was to see if there was any relation between Alii Vibrio fischeri (a specimen that life in warm around 20°C oceans) & Physarum Polycephalum (a specimen that life's in the forest in warm temperatures 20°C). In order to test this, I made an investigation around the two specimens. The second step was to cultivate both of them, sadly the extraction of the first specimen from an octopus didn't give results, so I redirected my project into teaching Physarum Polycephalum to adapt to salinity environments.

[[File:52h salinity medium.mp4|1500px]]

Here can be seen a video around how specimens that were teached to live in different salinity mediums behave when they have the possibility to move between different salinity mediums (Between 0% salinity and 0.5% salinity). It is a 52 h photage of Physarum Polycephalum in salinity medium.

'''Investigation around the specimens.'''

In the following links can be found the investigation around the two specimens, the medium that they need and more.

[[/Investigation around Aliivibrio Fischeri.]]

[[/Investigation around Physarum Polycephalum.]]

'''Specimens Mediums.'''

- Medium A.F:
*NaCl 30 g
*Glycerol 1 g
*Pepton (Bacxto-peptone) 10 g
*Meat extract 3 g
*Made up with distilled water to 1000 ml

- Medium P.P:
*100ml of distilled water
*2g of agar
*Leaflets

'''Experimentation with the specimens.'''

In the following links can be found the diary of work made with each specimen, the experiments that were made with each of them, the experimentation with some mediums and more.

[[/Diary of work: Alii Vibrio Fischeri]]

[[/Diary of work: Physarum Polycephalum]]

'''Conclusions:'''

After making the salinity experiment with the Physarum Polycephalum, I get into the conclusion that this specimen can learn to survive in mediums that have salt concentrations. It can learn to survive in them in a process of increasing the salinity, but not if it is situated in a salinity medium without previous teaching.

After the experiment comes to a lot of questions that can be solved with further experiments like; 1) how fast does it forget? 2) Does it remember after a period of sleep? 3) Is the sclerotium cultivation possible after a period of time?, 4) the specimen showed troubles to find the food in high levels of salinity, Does the levels of NaCl affect the ability of the specimen to find the food?

''Salinity Percentage Mediums: ''Strains P.p.P''
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

During the time that I adopted this specimen, I made many tests in order to get to know and understand the specimen. I'm also taking the class of "DIY Bio; doing things with biology", where I made many tests in terms of food, temperature, and light. This information can be found on the following websites:

- https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Paola_Stephania_Calder%C3%B3n/23.10.2018

-https://www.uni-weimar.de/kunst-und-gestaltung/wiki/GMU:DIY_Bio:_doing_things_with_biology/Paola_Stephania_Calder%C3%B3n/20.11.2018

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:58:17Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1500px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1500px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:57:50Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1200px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1200px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|1000px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|1000px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:57:17Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|1000px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|1000px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:56:56Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4|800px]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4|800px]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:56:35Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:56:21Z

Goji6184: /* PROJECT IDEA 2 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen ''(with coral shape)'' can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:55:58Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen (with coral shape) can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:55:40Z

Goji6184: /* PROJECT IDEA 2 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first approximation to isolate a specimen (with coral shape) can be seen in the following link.

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:55:11Z

Goji6184: /* PROJECT IDEA 2 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|800px]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:54:55Z

Goji6184:

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|400px]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:54:35Z

Goji6184: /* PROJECT IDEA 1 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|800px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|400px]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:54:22Z

Goji6184: /* PROJECT IDEA 1 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|700px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|400px]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:54:02Z

Goji6184: /* PROJECT IDEA 1 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|500px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|400px]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:53:46Z

Goji6184: /* PROJECT IDEA 2 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|400px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg|400px]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''

GMU:DIY Bio: doing things with biology/Paola Stephania Calderón

2019-03-27T23:53:17Z

Goji6184: /* PROJECT IDEA 2 */

==PROJECT IDEA 1==

The idea is to compare how the specimen ''Physarum Polycephalum'' grows in normal conditions, and how trained specimens behave when they have the possibility to change into a salinity environment.

A neutral medium was created, and a photo sequence video was created to see how the specimen grows in normal conditions, and another medium was created with stripes of different salinity mediums from 0% to .5% to see how the trained specimens behave. The following two videos show the results of both experiments.

[[File:62h normal medium.mp4]]

62h Physarum Polycephalum growth in neutral environment video

[[File:52h salinity medium.mp4]]

52h Physarum Polycephalum growth in salinity mediums video ''(from 0% to 0.5% salinity)''

Photogallery of Physarum Polycephalum living in different salinity porcentage mediums.
<gallery>
File:0% salinity.png
File:0.062% salinity.png
File:0.125% salinity.png
File:0.25% salinity.png
File:0.5% salinity.png
</gallery>

''The initial experiment of teaching Physarum Polycephalum to live in salinity mediums was made in the class GMU:Experimental Microbiology, the experiment and results can be seen in the following link:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n/Project:_Specimen_%27%27Physarum_Polycephalum%27%27.]

''All experiments made in the class "Experimental Microbiology" can be seen at:'' [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/Paola_S._Calder%C3%B3n]

RELATED EXPERIMENTS

>> ''First approximation to the organism''

Three different kinds of samples were made the same day, with the same conditions and the same medium, but they develop in different ways, the following link shows the diary of work for each experiment that was made. From this three experiments, I learned how to cultivate physarum/polycephalum how it grows, the type of conditions that it needs (medium) to grow up and the temperature that it also needs to grow.

Datasheet of ''Physarum Polycephalum:''

[[File:DATA sheet Physarum Polycephalum.jpg|400px]]

Links to further experiments with Physarum Polycephalum:

[[/Farming 1]] ''Farming from a living sample''

[[/Farming 2]] ''Farming from a sleeping sample''

[[/Farming 3]] ''Farming from a combined sample''

>> ''Second approximation to the organism''

This link contains the knowledge, results and the experiments made in order to create an ideal medium for faster growth base in the previous investigation of Caicedo.

[[/20.11.2018]] ''Growing Polycephalum/physarum from Caicedos investigation''

==PROJECT IDEA 2==
The first approximation is to recreated and find a method in order to cultivate a microorganism with a coral shape.

The project idea is to create a guide based on the recreation of the experiment made before with the kombucha. The following guide includes the information of the chemical compounds to create the medium, the instruments needed to recreate the experiment, instructions before starting to work and instructions of how to create the medium and insolate the gluconacetobacter.

[[File:Gluconacetobacter lab guide.jpg]]

The diary of work made in the first aproximation to insolate a specimen (with coral shape) can be seen in the following link.

[[File:Bio-07.png|400px]]

[[/30.10.2018]] ''Separation of one specimen - kombucha'' Link to the first approximation to this experiment.

==FURTHER EXPERIMENTS==

During this class, a lot of experiments were made in order to understand the lab rules, learn different techniques inside the lab, for example how to grow different kinds of specimens, observation under the microscope, and others.

''These experiments can be seen in the following links:''

[[/16.10.2018]] ''Introduction of the course''

[[/06.11.2018a]] ''Growing Mycelium''

[[/06.11.2018b]] ''Capturing electric potentials in organic matter''

[[/18.12.2018]] ''Observation of specimens under the microscope''

[[/22.01.2019]] ''Gelelectrophoresis''