Medien Wiki - User contributions [en]

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-14T16:03:45Z

Fufe2685: /* 6th step: Incubate at 30° for 5-7 days */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|800px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:''' Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|800px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000 (2)
</gallery>

'''Observations:'''
There has been overgrowing a hybrid of organisms, but assumingly the pinky/white powdery dots are streptomyces colonies.Such colonies are mostly to be found on the 1:100 and 1:1000 dilution plates. In this case, creating subcultures is the best solution, in order to get a unified-type of biotope in the petri dish!

Only one sample has been contaminated, as it was the first one to get done during the isolation process.

==FINAL DAY MICROSCOPICAL VIEWS==
[[File:Micro2.png|800px]]

==Insight==
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

'''...take a minute to think and ''praise the genus of good welfare.'''''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T19:22:06Z

Fufe2685: /* 6-DAY OUTCOMES */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|800px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|800px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000 (2)
</gallery>

'''Observations:'''
There has been overgrowing a hybrid of organisms, but assumingly the pinky/white powdery dots are streptomyces colonies.Such colonies are mostly to be found on the 1:100 and 1:1000 dilution plates. In this case, creating subcultures is the best solution, in order to get a unified-type of biotope in the petri dish!

Only one sample has been contaminated, as it was the first one to get done during the isolation process.

==FINAL DAY MICROSCOPICAL VIEWS==
[[File:Micro2.png|800px]]

==Insight==
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

'''...take a minute to think and ''praise the genus of good welfare.'''''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T19:17:56Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|800px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|800px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000 (2)
</gallery>

'''Observations:'''
There has been overgrowing a hybrid of organisms, but assumingly the pinky/white powdery dots are streptomyces colonies. Such colonies are mostly to be found on the 1:100 and 1:1000 dilution plates. Only one sample has been contaminated, as it was the first one to get done during the isolation process.

==FINAL DAY MICROSCOPICAL VIEWS==
[[File:Micro2.png|800px]]

==Insight==
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

'''...take a minute to think and ''praise the genus of good welfare.'''''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T19:06:15Z

Fufe2685: /* 6-DAY OUTCOMES */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|800px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|800px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000 (2)
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
[[File:Micro2.png|800px]]

==Insight==
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

'''...take a minute to think and ''praise the genus of good welfare.'''''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T16:19:37Z

Fufe2685: /* Insight */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
::[[File:Micro2.png]]

==Insight==
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, they produce the two-thirds of all existent antibiotics. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

'''...take a minute to think and ''praise the genus of good welfare.'''''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T16:12:55Z

Fufe2685: /* Insight */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
::[[File:Micro2.png]]

==Insight==
'''Streptomyces''' species are the most abundant source of antibiotics. More specifically, of the two-thirds of all antibiotics of the world. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

'''...take a minute to think and ''praise the genus of good welfare.'''''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T16:12:29Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
::[[File:Micro2.png]]

==Insight==
Streptomyces species are the most abundant source of antibiotics. More specifically, of the two-thirds of all antibiotics of the world. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill...

...take a minute to think and ''praise the genus of good welfare.''

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T08:06:19Z

Fufe2685: /* Insight */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
::[[File:Micro2.png]]

==Insight==
Streptomyces species are the most abundant source of antibiotics. More specifically, of the two-thirds of all antibiotics of the world. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill... take a minute to think and praise the genus of

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:59:11Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
::[[File:Micro2.png]]

==Insight==
Streptomyces species are the most abundant source of antibiotics. More specifically, of the two-thirds of all antibiotics of the world. Except that, this genus produces a rife scope of bioactive compounds. It usually inhabits the soil and plays a significant role in decomposing.

So, next time when you get ill, decide to take another pill, in order to re-feel the thrill... try to

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:51:05Z

Fufe2685: /* FINAL DAY MICROSCOPICAL VIEWS */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
::[[File:Micro2.png]]

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:49:56Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>

==FINAL DAY MICROSCOPICAL VIEWS==
:::[[File:Micro2.png]]

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

File:Micro2.png

2016-07-13T07:49:43Z

Fufe2685: Final Day microscope

== Summary ==
Final Day microscope
== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

File:Sample4.png

2016-07-13T07:42:19Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:41:20Z

Fufe2685: /* 3-DAY OUTCOMES */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
<gallery>
File:After3days.png
File:After3dayss.png
</gallery>

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:40:18Z

Fufe2685: /* 3-DAY OUTCOMES */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
::[[File:After3days.png|400px]] [[File:After3dayss.png|400px]]

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

File:Sample2.png

2016-07-13T07:39:24Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

File:Sample3.png

2016-07-13T07:38:52Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:38:24Z

Fufe2685: /* 6th step: Incubate at 30° for 5-7 days */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.

[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]]

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:37:32Z

Fufe2685: /* 6th step: Incubate at 30° for 5-7 days */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
'''Important:'''Use parafilm to fortify the covers of each petri dish, in order to avoid evaporation, as it can lead to contamination.
[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----

==3-DAY OUTCOMES==
::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]]

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:35:32Z

Fufe2685: /* 6-DAY OUTCOMES */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----
==3-DAY OUTCOMES==
::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]]

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==
<gallery>
File:Sample1.png|1:10
File:Sample2.png|1:100
File:Sample3.png|1:1000
File:Sample4.png|1:1000
</gallery>
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

File:Sample1.png

2016-07-13T07:35:10Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:25:49Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----
==3-DAY OUTCOMES==
::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]]

'''Observations:'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.

==6-DAY OUTCOMES==

----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:23:43Z

Fufe2685: /* 3-day outcomes */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----
==3-day outcomes==
::[[File:After3days.png|500px]] [[File:After3dayss.png|500px]]

'''OBSERVATIONS'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The small white powdery looks like a good sign.
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:21:24Z

Fufe2685: /* 3-day outcomes */

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----
==3-day outcomes==
::[[File:After3days.png|600px]] [[File:After3dayss.png|600px]]

'''OBSERVATIONS'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The white little 'dots' are a good sign.
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:20:40Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
[[File:Incubation.png|400px]]

Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----
==3-day outcomes==
::[[File:After3days.png|200px]] [[File:After3dayss.png|200px]]

'''OBSERVATIONS'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The white little 'dots' are a good sign.
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T07:19:48Z

Fufe2685:

=Spot the antibiotics producer: STREPTOMYCES=
[[File:Micro.png|650px]]
----

''Antibiotics''.

The human gets ill, goes downhill, forgets the dollar bill, ''takes a pill'', regains will, re-feels the thrill.

It’s simply the recuperation cycle.

But, do you ever think about the fundamentals of having the chance of recuperation from infections?

Sure.

It’s thanks to the pill - the antibiotic.

Good job!

Now, zoom in deeper…

Do you actually know where this savior comes from?

Think no further than ''the soil''.

''Streptomyces''.
----

===ISOLATION OF STREPTOMYCES===
====1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment====
<gallery>
File:Soil1.png
File:Soil.png
</gallery>

====2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min====
<gallery>
File:NACL.png
File:Solution .png
</gallery>

====3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it====
<gallery>
File:Diluting.png
File:Dilutions&Solution.png
</gallery>

====4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes====
<gallery>
File:Mannitol.png
File:Plates.png
</gallery>

''MSA RECIPE:''

In a 250 mL conical flask mix:
*2g Agar
*2g Mannitol
*2g Soya flour (Holland and Barrett)
*10 mM CaCl2
*100 mL Deionised water
<tt>
Medium alternative: Inorganic Salt Starch Agar (ISSA)
</tt>

====5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA)====
<gallery>
File:Put.png
File:Spread.png
</gallery>
<tt>
The loop used for spreading the solution on the medium (shown on the 2nd photo) is a DIY loop, created from paper clips
</tt>

====6th step: Incubate at 30° for 5-7 days====
[[File:Incubation.png|400px]]
Optional: Supplementation with cyclohexamide (50 μg/ml) and nystatin (50 μg/ml) to further remove competing bacteria
----
==3-day outcomes==
::[[File:After3days.png|200px]] [[File:After3dayss]]

'''OBSERVATIONS'''
The no-dilution petri dish shows a tendency towards contamination. Other petri dishes seem on the right track by far. The white little 'dots' are a good sign.
----

'''Inspiration''':
http://annadumitriu.tumblr.com/ - Anna Dumitriu
https://exploringtheinvisible.com/ - Dr. Simon Park

File:After3dayss.png

2016-07-13T07:19:05Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

File:After3days.png

2016-07-13T07:16:39Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:53:04Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:52:07Z

Fufe2685: /* 5th step: Plate an aliquot of 0.1ml of each dilution on the medium (MSA) */

File:Spread.png

2016-07-13T06:49:09Z

Fufe2685: Spread the solution on the medium with a loop

== Summary ==
Spread the solution on the medium with a loop
== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

File:Put.png

2016-07-13T06:48:03Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:45:59Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:42:43Z

Fufe2685: /* 1st step: Air dry the soil samples for 3-4 h at 45° for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment */

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:40:42Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:39:37Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:38:23Z

Fufe2685: /* 4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes */

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:37:05Z

Fufe2685: /* 3rd step: Prepare 3 dilutions (1:10, 1:100, 1:1000) out of it */

File:Dilutions&Solution.png

2016-07-13T06:36:55Z

Fufe2685: 3 dilutions along with the main solution

== Summary ==
3 dilutions along with the main solution
== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

File:Diluting.png

2016-07-13T06:35:58Z

Fufe2685:

== Summary ==

== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:31:19Z

Fufe2685: /* 2nd step:Dilute 1-3g of soil in 10 ml saline solution (NaCl 0,9%) and let the solution stand for 15 min */

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:30:34Z

Fufe2685:

File:NACL.png

2016-07-13T06:30:17Z

Fufe2685: saline solution 0,9

== Summary ==
saline solution 0,9
== Copyright status: ==

== Source: ==

File:NACL.jpg

2016-07-13T06:27:55Z

Fufe2685: saline solution 0,9%

== Summary ==
saline solution 0,9%
== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-13T06:25:23Z

Fufe2685:

File:Soil1.png

2016-07-13T06:24:24Z

Fufe2685: pure soil

== Summary ==
pure soil
== Copyright status: ==

== Licensing ==
{{self|c}}
== Source: ==

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-12T22:51:05Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-12T22:47:04Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-12T22:44:28Z

Fufe2685:

GMU:Bioelectronics, aesthetics and other interesting things/Trina Ukmata

2016-07-12T22:37:50Z

Fufe2685: