GMU:Flagelates, Nematodes, and I/Introduction to tools and the framework

Tools and Toolkits

Students will be provided with video tutorials and toolkits containing tools. Available 5 toolkits / each.

Euglena gracilis

various glassware (6 units)
Euglena gracilis
Chemicals
mini scale
small pipette

Heterohabditis bacteriophora

Petri dishes (5 units)
3d Labyrinth
agar-agar
dried Heterohabditis bacteriophora
Digital microskope

Organisms

Euglena gracilis

“Euglena are found in fresh and salt waters. They are often abundant in quiet inland waters where they may bloom in numbers sufficient to color the surface of ponds and ditches green (E. viridis) or red (E. sanguinea).” (wikipedia)

“Most species of Euglena have photosynthesizing chloroplasts within the body of the cell, which enable them to feed by autotrophy, like plants. However, they can also take nourishment heterotrophically, like animals.” (wikipedia)

Euglena. Source: wikimedia.org]]

Heterorhabditis bacteriophora

"Heterorhabditis bacteriophora is a species of entomopathogenic nematode known commonly as beneficial nematodes. They are microscopic and are used in gardening as a form of biological pest control. They are used to control ants, fleas, moths, beetles, flies, weevils, and other pests." (wikipedia)

"To reproduce the nematodes release Photorhabdus bacteria from their digestive tract thus killing these pests, then using the cadaver to grow and reproduce." (wikipedia)

Heterorhabditis bacteriophora nematodes emerging from a greater wax moth. Source: wikimedia.org

Manuals

Euglena gracilis

Preparation medium for Euglena
Bioconvection, File:Mikrokosmos_85_2_P_21-30-1.pdf
Euglena and light, File:euglena-light-experiment.pdf